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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08730 [old locus tag: NWMN_1557 ]
  • pan locus tag?: SAUPAN004274000
  • symbol: NWMN_RS08730
  • pan gene symbol?: folC
  • synonym:
  • product: bifunctional folylpolyglutamate synthase/dihydrofolate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS08730 [old locus tag: NWMN_1557 ]
  • symbol: NWMN_RS08730
  • product: bifunctional folylpolyglutamate synthase/dihydrofolate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1721191..1722462
  • length: 1272
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1721191..1722462) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATTACCTAGAGAGCTTGTATTGGATACACGAAAGAACTAAATTTGGCATCAAACCA
    GGTGTTAAACGTATGGAATGGATGCTAGCACAATTTAATAATCCTCAAAATAACATTAAG
    GGTATTCATGTAGGTGGCACAAATGGTAAAGGCTCTACAGTTGCTTACCTTAGAACAGCT
    TTAGTTGAAAATGGTTATGAAGTAGGTACATTTACGTCGCCGTTTATTGAAACATTTAAT
    GAACGAATTAGTCTAAATGGTGTGCCAATATCAAATGACGCTATTGTAGAATTAGTATCA
    CGTATTAAACCAGTAAGTGAAATGATGGAACGTGAAACAGATTTAGGTGTTGCAACTGAA
    TTCGAAATAATCACAGCGATGATGTTTTTATATTTTGGTGAAATACATCCTGTTGATTTT
    GTCATTGTTGAGGCTGGATTGGGTATAAAGAACGATTCGACAAATGTCTTTACACCGGTT
    TTATCAATCTTAACTAGTATCGGTCTAGACCATACAGATATTTTAGGTGGTACTTATCTA
    GATATTGCTAGGGATAAAGGCGCGATTATAAAGCCTAACGTTCCAGTGATATATGCTGTT
    AAAAATGAAGATGCATTAAAATATGTTCGTGAACGCGCAATTGAACAACATGCAAAGCCA
    ATTGAATTAGATAGAGAAATTGTTGTTGTATCGCAAAATGATGAATTTACTTACCGTTAT
    AAAGATTATGAATTAGAAACAATCATTTTAAGCATGTTAGGTGAACATCAGAAACAAAAT
    GCTGCATTAGCCATAACAGCTCTTATTGAATTAAATGAACAAGGATTAATTGAATTAGAT
    TTCAATAAGATGATAGACGGTATTGAATCAGTTCGTTGGACTGGACGTATTGAGCAGGTG
    CATGACAAACCTTTAATCATTTTGGATGGCGCACATAATTCAGAGAGTATAGATGCTCTA
    ATTGATACAATTAAACAGTACCATGATAAAGAAAAAGTAGATATTTTGTTCTCAGCAATA
    AACGGAAAACCGATTAACGAGATGGTCAAACATTTAAGTTTAATTGCGCATACGTTTTAT
    GCAACTGAATTTGATTTTCCGAAAGCGTTACGCAAAGAAGAAATTGTAGGTAGTATTGAA
    AATGATGAAATACAATTAGTAGATGACTACGTTGAATTTATAAAAAATTATCAAGGTGAT
    ACATTAGTAATTACCGGTAGTCTGTATTTCATAAGTGAAGTTAAATCAAAATTAGACTTT
    GAAGCATTATAA
    60
    120
    180
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    480
    540
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    720
    780
    840
    900
    960
    1020
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    1140
    1200
    1260
    1272

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS08730 [old locus tag: NWMN_1557 ]
  • symbol: NWMN_RS08730
  • description: bifunctional folylpolyglutamate synthase/dihydrofolate synthase
  • length: 423
  • theoretical pI: 4.55771
  • theoretical MW: 47856.3
  • GRAVY: -0.0959811

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidbifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 376.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 55)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 41.2)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 35.7)
    Cell structureCell envelopeOtherpoly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 19.7)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 15.1)
  • TheSEED:
  • PFAM:
    no clan definedMur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 42.8)
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 27.5)
    ACT (CL0070) ALS_ss_C; Small subunit of acetolactate synthase (PF10369; HMM-score: 13.8)
    P-loop_NTPase (CL0023) AAA_14; AAA domain (PF13173; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 42
    • Cmax: 0.115
    • Cmax_pos: 25
    • Smax: 0.194
    • Smax_pos: 37
    • Smean: 0.135
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447031090 NCBI
  • RefSeq: WP_001108346 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPVLSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGDTLVITGSLYFISEVKSKLDFEAL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS08730 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]