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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS10725 [old locus tag: NWMN_1866 ]
  • pan locus tag?: SAUPAN004996000
  • symbol: NWMN_RS10725
  • pan gene symbol?: pmtD
  • synonym:
  • product: membrane protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS10725 [old locus tag: NWMN_1866 ]
  • symbol: NWMN_RS10725
  • product: membrane protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2079452..2080192
  • length: 741
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (2079452..2080192) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1866

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAGAATTTTAAATTTAGTTAAGTATGATTTTTATAGTATATTTAAAAGTCCTTTAACA
    TATTTAGCGATACTAGTCGTATCTAGTTTGATTGCAACTCAAAGTATACTTATGGCAAAT
    TCGATGGATAACCCGAAACATATTATTGTCTATGGATCTGTATTTGCTGCAGCAAAATGG
    TTATTGTTAATAATTGGATTAATGTTTGTTGTTAAGACAATTACGCGTGATTTTTCACAA
    GGTACAATTCAACTATATATGAGTAAAGTTAAAACACGCGTTGGATACATTATTTCGAAA
    ACAATTTCAATTATTTTAATTTCAATATTATTTGCATTAATTCATTATGTGATTTTGATT
    GTTGTGCAGGCATCTAGTAATGGAAAAAATTTGGCGTTTTCTAAATATGTAGATAATTTA
    TGGTTCTTCCTAATCTTTTTACTATTCTTTGGCTTGTTTTTATTCTTAATCACACTTGCA
    TCACAAAAAACAGCAATGATATTTTCATTAGGTGTATTTTTAGTACTCATTGTACCGTTT
    ATTAAACCTTTTATTACATTTATCCCAAGATACGGTGAAAAAGTTTTAGATGCTTTTGAT
    TATATCCCTTTTGCTTACTTAACTGATAAAATGATTAGCTCTAACTTTGATTTTAGCAAT
    TGGCAATGGGTAATTTCATTAGGTTCTATAGTGATATTCTTCATTTTGAATATCTTATAT
    GTCGCTAAAAAAGACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    741

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS10725 [old locus tag: NWMN_1866 ]
  • symbol: NWMN_RS10725
  • description: membrane protein
  • length: 246
  • theoretical pI: 10.1289
  • theoretical MW: 28138.8
  • GRAVY: 1.00772

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ABC-2 (CL0181) ABC2_membrane_2; ABC-2 family transporter protein (PF12679; HMM-score: 37.4)
    and 2 more
    ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 21.9)
    ABC2_membrane_4; ABC-2 family transporter protein (PF12730; HMM-score: 19.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.070487
    • TAT(Tat/SPI): 0.000757
    • LIPO(Sec/SPII): 0.043602
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDNPKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTISIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLASQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDFSNWQWVISLGSIVIFFILNILYVAKKDI

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • data available for N315

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]