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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS11340 [old locus tag: NWMN_1965 ]
  • pan locus tag?: SAUPAN005309000
  • symbol: NWMN_RS11340
  • pan gene symbol?: leuC
  • synonym:
  • product: 3-isopropylmalate dehydratase large subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS11340 [old locus tag: NWMN_1965 ]
  • symbol: NWMN_RS11340
  • product: 3-isopropylmalate dehydratase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 2176954..2178324
  • length: 1371
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2176954..2178324) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGC
    GAACCGCAACTATTATACATTGATTTACACCTTATACATGAAGTTACTTCTCCTCAAGCA
    TTTGAAGGACTTAGGCTTCAAAACAGAAAATTAAGACGCCCAGATTTAACATTTGCAACA
    CTCGATCACAATGTTCCTACTATTGATATATTCAATATTAAAGATGAAATTGCAAACAAA
    CAAATCACAACATTACAAAAAAACGCCATAGATTTTGGGGTGCATATTTTTGATATGGGT
    TCTGATGAACAAGGTATTGTTCACATGGTAGGACCTGAGACAGGACTTACACAGCCTGGC
    AAGACAATCGTTTGTGGTGACTCTCACACAGCAACACATGGAGCATTTGGTGCTATTGCA
    TTTGGAATTGGAACAAGTGAAGTTGAACATGTTTTCGCAACTCAAACGCTATGGCAAACA
    AAACCCAAAAACTTAAAAATCGATATTAATGGTACCTTACCAACAGGCGTCTATGCTAAG
    GACATTATTCTGCATTTAATTAAAACGTATGGTGTTGACTTTGGTACAGGCTATGCTTTG
    GAATTTACTGGCGAAACAATTAAAAACCTTTCAATGGATGGTCGAATGACTATTTGTAAC
    ATGGCTATCGAAGGTGGTGCCAAATACGGCATAATCCAACCTGATGATATAACATTTGAA
    TATGTTAAAGGGAGACCATTTGCCGATAACTTCGCTAAATCAGTTGATAAGTGGCGTGAG
    CTATATTCTGATGACGACGCGATATTTGATCGTGTAATTGAACTTGATGTTTCAACATTA
    GAACCACAAGTGACATGGGGAACTAATCCTGAAATGGGTGTTAATTTCAGTGAACCATTC
    CCTGAAATCAATGATATCAACGATCAACGTGCGTATGATTATATGGGGTTAGAACCAGGT
    CAAAAAGCTGAAGACATCGACTTAGGGTATGTTTTTCTCGGTTCATGTACAAATGCTAGA
    CTATCAGATTTGATTGAAGCTAGTCATATTGTTAAAGGAAATAAAGTTCATCCAAATATT
    ACAGCTATTGTCGTACCAGGTTCTCGTACAGTAAAAAAAGAAGCAGAAAAATTAGGTCTA
    GATACTATCTTTAAAAATGCAGGATTTGAATGGCGTGAACCAGGATGTTCAATGTGTTTA
    GGCATGAATCCTGACCAAGTACCTGAGGGCGTACATTGTGCATCTACAAGTAATCGAAAC
    TTTGAAGGACGACAAGGCAAAGGTGCAAGAACACATTTAGTATCCCCTGCTATGGCAGCA
    GCAGCAGCTATTCATGGTAAATTTGTGGACGTAAGAAAGGTGGTTGTTTAA
    60
    120
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    1140
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    1260
    1320
    1371

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS11340 [old locus tag: NWMN_1965 ]
  • symbol: NWMN_RS11340
  • description: 3-isopropylmalate dehydratase large subunit
  • length: 456
  • theoretical pI: 5.56699
  • theoretical MW: 50325.9
  • GRAVY: -0.230263

Function[edit source | edit]

  • reaction:
    EC 4.2.1.33?  ExPASy
    3-isopropylmalate dehydratase(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydratase, large subunit (TIGR00170; EC 4.2.1.33; HMM-score: 695.6)
    homoaconitate hydratase family protein (TIGR01343; HMM-score: 340.7)
    3-isopropylmalate dehydratase, large subunit (TIGR02086; HMM-score: 305.7)
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydratase, large subunit (TIGR02083; EC 4.2.1.33; HMM-score: 287.2)
    MetabolismEnergy metabolismTCA cycleputative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 206.5)
    MetabolismAmino acid biosynthesisAspartate familyhomoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 151.5)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 144.5)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 105.1)
    2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 90.4)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase 2 (TIGR00117; EC 4.2.1.3; HMM-score: 71.6)
  • TheSEED:
  • PFAM:
    no clan definedAconitase; Aconitase family (aconitate hydratase) (PF00330; HMM-score: 491.4)
    DUF521; Protein of unknown function (DUF521) (PF04412; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.097
    • Ymax_pos: 41
    • Cmax: 0.135
    • Cmax_pos: 41
    • Smax: 0.094
    • Smax_pos: 66
    • Smean: 0.066
    • D: 0.085
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446453968 NCBI
  • RefSeq: WP_000531823 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTKPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS11340 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]