From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2048 [new locus tag: SACOL_RS10695 ]
  • pan locus tag?: SAUPAN005309000
  • symbol: leuC
  • pan gene symbol?: leuC
  • synonym:
  • product: isopropylmalate isomerase large subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2048 [new locus tag: SACOL_RS10695 ]
  • symbol: leuC
  • product: isopropylmalate isomerase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 2108571..2109941
  • length: 1371
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGC
    GAACCGCAACTATTATACATTGATTTACACCTTATACATGAAGTTACTTCTCCTCAAGCA
    TTTGAAGGACTTAGGCTTCAAAACAGAAAATTAAGACGCCCAGATTTAACATTTGCAACA
    CTCGATCACAATGTTCCTACTATTGATATATTCAATATTAAAGATGAAATTGCAAACAAA
    CAAATCACAACATTACAAAAAAACGCCATAGATTTTGGGGTGCATATTTTTGATATGGGT
    TCTGATGAACAAGGTATTGTTCACATGGTAGGACCTGAGACAGGACTTACACAGCCTGGC
    AAGACAATCGTTTGTGGTGACTCTCACACAGCAACACATGGAGCATTTGGTGCTATTGCA
    TTTGGAATTGGAACAAGTGAAGTTGAACATGTTTTCGCAACTCAAACGCTATGGCAAACA
    AAACCCAAAAACTTAAAAATCGATATTAATGGTACCTTACCAACAGGCGTCTATGCTAAG
    GACATTATTCTGCATTTAATTAAAACGTATGGTGTTGACTTTGGTACAGGCTATGCTTTG
    GAATTTACTGGCGAAACAATTAAAAACCTTTCAATGGATGGTCGAATGACTATTTGTAAC
    ATGGCTATCGAAGGTGGTGCCAAATACGGCATAATCCAACCTGATGATATAACATTTGAA
    TATGTTAAAGGGAGACCATTTGCCGATAACTTCGCTAAATCAGTTGATAAGTGGCGTGAG
    CTATATTCTGATGACGACGCGATATTTGATCGTGTAATTGAACTTGATGTTTCAACATTA
    GAACCACAAGTGACATGGGGAACTAATCCTGAAATGGGTGTTAATTTCAGTGAACCATTC
    CCTGAAATCAATGATATCAACGATCAACGTGCGTATGATTATATGGGGTTAGAACCAGGT
    CAAAAAGCTGAAGACATCGACTTAGGGTATGTTTTTCTCGGTTCATGTACAAATGCTAGA
    CTATCAGATTTGATTGAAGCTAGTCATATTGTTAAAGGAAATAAAGTTCATCCAAATATT
    ACAGCTATTGTCGTACCAGGTTCTCGTACAGTAAAAAAAGAAGCAGAAAAATTAGGTCTA
    GATACTATCTTTAAAAATGCAGGATTTGAATGGCGTGAACCAGGATGTTCAATGTGTTTA
    GGCATGAATCCTGACCAAGTACCTGAGGGCGTACATTGTGCATCTACAAGTAATCGAAAC
    TTTGAAGGACGACAAGGCAAAGGTGCAAGAACACATTTAGTATCCCCTGCTATGGCAGCA
    GCAGCAGCTATTCATGGTAAATTTGTGGACGTAAGAAAGGTGGTTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2048 [new locus tag: SACOL_RS10695 ]
  • symbol: LeuC
  • description: isopropylmalate isomerase large subunit
  • length: 456
  • theoretical pI: 5.56699
  • theoretical MW: 50325.9
  • GRAVY: -0.230263

Function[edit source | edit]

  • reaction:
    EC 4.2.1.33?  ExPASy
    3-isopropylmalate dehydratase(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydratase, large subunit (TIGR00170; EC 4.2.1.33; HMM-score: 695.6)
    homoaconitate hydratase family protein (TIGR01343; HMM-score: 340.7)
    3-isopropylmalate dehydratase, large subunit (TIGR02086; HMM-score: 305.7)
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydratase, large subunit (TIGR02083; EC 4.2.1.33; HMM-score: 287.2)
    MetabolismEnergy metabolismTCA cycleputative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 206.5)
    MetabolismAmino acid biosynthesisAspartate familyhomoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 151.5)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 144.5)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 105.1)
    2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 90.4)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase 2 (TIGR00117; EC 4.2.1.3; HMM-score: 71.6)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsBranched-Chain Amino Acid Biosynthesis 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) 
    Branched-chain amino acidsLeucine Biosynthesis 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) 
  • PFAM:
    no clan definedAconitase; Aconitase family (aconitate hydratase) (PF00330; HMM-score: 491.4)
    DUF521; Protein of unknown function (DUF521) (PF04412; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.097
    • Ymax_pos: 41
    • Cmax: 0.135
    • Cmax_pos: 41
    • Smax: 0.094
    • Smax_pos: 66
    • Smean: 0.066
    • D: 0.085
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTKPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 985 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 8.08 h [6]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]