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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS11680 [old locus tag: NWMN_2021 ]
  • pan locus tag?: SAUPAN005408000
  • symbol: NWMN_RS11680
  • pan gene symbol?: prmC
  • synonym:
  • product: protein-(glutamine-N5) methyltransferase, release factor-specific

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS11680 [old locus tag: NWMN_2021 ]
  • symbol: NWMN_RS11680
  • product: protein-(glutamine-N5) methyltransferase, release factor-specific
  • replicon: chromosome
  • strand: -
  • coordinates: 2237415..2238251
  • length: 837
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2237415..2238251) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGTGAATTATAAAGAAAAGTTAGATGAAGCAATTCATTTAACACAACAAAAAGGGTTT
    GAACAAACACGAGCTGAATGGTTAATGTTAGATGTATTTCAATGGACGCGTACGGACTTT
    GTAGTCCACATGCATGATGATATGCCGAAAGCGATGATTATGAAGTTCGACTTAGCATTA
    CAACGTATGTTATTAGGAGAGCCTATACAGTATATAGTTGGCTTTGCCTCATTTTATGGT
    AGAACGTTTGATGTAAACTCAAATTGTTTGATACCAAGACCTGAAACTGAAGAAGTAATG
    TTGCATTTCTTACAACAGTTAGAAGATGATGCAACAATCGTAGATATCGGAACGGGTAGT
    GGTGTACTTGCAATTACTTTGAAATGTGAAAAGCCGGATTTAAATGTTATTGCTACTGAT
    ATTTCACTTGAAGCAATGAATATGGCTCGTAATAATGCTGAGAAGCATCAATCACAAATA
    CAATTTTTAACAGGGGATGCATTAAAGCCCTTAATTAATGAAGGTATCAAGTTGAACGGC
    TTGATATCTAATCCACCATATATAGATGAAAAAGATATGGTTACGATGTCTCCAACGGTT
    ACGAGATTCGAACCACATCAGGCATTGTTTGCAGATAACCATGGATATGCTATTTATGAA
    TCAATCATTGAAGATTTACCTCACGTTATGGAAAAAGGCAGCCCAGTTGTTTTTGAAATT
    GGTTACAATCAAGGTGAGGCACTTAAATCAATAATTTTAAATAAATTTCCTGACAAAAAA
    ATCGACATTATTAAAGATATAAATGGCCACGATCGAATCGTCTCATTTAAATGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    837

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS11680 [old locus tag: NWMN_2021 ]
  • symbol: NWMN_RS11680
  • description: protein-(glutamine-N5) methyltransferase, release factor-specific
  • length: 278
  • theoretical pI: 4.76327
  • theoretical MW: 31730.3
  • GRAVY: -0.191727

Function[edit source | edit]

  • reaction:
    EC 2.1.1.297?  ExPASy
    Peptide chain release factor N5-glutamine methyltransferaseS-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
  • TIGRFAM:
    Genetic information processingProtein fateProtein modification and repairprotein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 259.5)
    Genetic information processingProtein fateProtein modification and repairmethyltransferase, HemK family (TIGR00536; HMM-score: 207)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationprotein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 114.9)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationputative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 71.4)
    Unknown functionEnzymes of unknown specificityputative methylase (TIGR00537; HMM-score: 47.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 32.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 31.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinmalonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 28.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllmagnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 27.4)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 26.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 23.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 23)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 20.7)
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 18.7)
    Genetic information processingProtein fateProtein modification and repairprotein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 15.1)
    Genetic information processingProtein synthesistRNA and rRNA base modification16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 13.9)
    Genetic information processingTranscriptionRNA processing3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 13.9)
    Genetic information processingProtein synthesistRNA and rRNA base modification3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 13.9)
    2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 13.2)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesputative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 11.6)
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (uracil-5-)-methyltransferase RumB (TIGR02085; EC 2.1.1.189; HMM-score: 10.8)
  • TheSEED:
  • PFAM:
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 57.7)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 34.6)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 34.4)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 34.1)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 31.8)
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 26)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 24)
    Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 23.9)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 22)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 21.5)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 19.7)
    Methyltransf_2; O-methyltransferase (PF00891; HMM-score: 19.6)
    Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 19.1)
    GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 17.5)
    CmcI; Cephalosporin hydroxylase (PF04989; HMM-score: 16.8)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 16.6)
    TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 16.1)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 13.8)
    CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.098
    • Ymax_pos: 68
    • Cmax: 0.106
    • Cmax_pos: 26
    • Smax: 0.121
    • Smax_pos: 56
    • Smean: 0.059
    • D: 0.083
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446170880 NCBI
  • RefSeq: WP_000248735 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS11680 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]