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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS13625 [old locus tag: NWMN_2364 ]
  • pan locus tag?: SAUPAN006027000
  • symbol: NWMN_RS13625
  • pan gene symbol?: cntA
  • synonym:
  • product: nickel ABC transporter, nickel/metallophore periplasmic binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS13625 [old locus tag: NWMN_2364 ]
  • symbol: NWMN_RS13625
  • product: nickel ABC transporter, nickel/metallophore periplasmic binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2600688..2602286
  • length: 1599
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2600688..2602286) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    ATGAGAAAACTAACTAAAATGAGTGCAATGTTACTTGCATCAGGGCTAATTTTAACTGGT
    TGTGGCGGTAATAAAGGTTTAGAGGAGAAAAAAGAAAACAAGCAATTAACGTATACGACG
    GTTAAAGATATCGGTGATATGAATCCGCATGTTTACGGTGGATCAATGTCTGCTGAAAGT
    ATGATATACGAGCCGCTTGTACGTAACACGAAAGATGGTATTAAGCCTTTACTAGCTAAA
    AAGTGGGATGTGTCTGAAGATGGGAAGACATACACGTTCCATTTGAGAGATGACGTTAAA
    TTCCATGATGGTACGCCATTTGATGCTGACGCAGTTAAGAAAAATATTGACGCAGTTCAA
    GAAAACAAAAAATTGCATTCTTGGTTAAAGATTTCGACATTAATTGACAATGTTAAAGTT
    AAAGATAAGTACACGGTTGAATTGAATTTGAAAGAAGCATATCAACCTGCATTGGCTGAA
    TTAGCGATGCCTCGTCCATATGTATTTGTGTCTCCAAAAGACTTTAAAAACGGTACAACA
    AAAGATGGCGTTAAAAAGTTCGATGGTACTGGTCCATTTAAATTAGGTGAACACAAAAAA
    GATGAGTCTGCAGACTTTAACAAAAATGATCAATACTGGGGCGAAAAGTCTAAACTTAAC
    AAAGTACAAGCAAAAGTAATGCCTGCTGGTGAAACAGCATTCCTATCAATGAAAAAAGGT
    GAAACGAACTTTGCCTTCACAGATGATAGAGGTACAGATAGCTTAGACAAAGACTCTTTA
    AAACAATTGAAAGATACAGGTGACTATCAAGTTAAGCGTAGTCAACCTATGAATACGAAA
    ATGTTAGTTGTCAATTCTGGTAAAAAAGATAACGCTGTGAGTGACAAAACAGTCAGACAA
    GCGATTGGTCATATGGTAAACAGAGATAAAATTGCCAAAGAAATTTTAGATGGTCAAGAA
    AAACCAGCAACTCAATTATTTGCGAAAAATGTAACAGACATTAATTTCGATATGCCAACA
    CGTAAGTATGACCTTAAAAAAGCAGAATCATTATTAGATGAAGCTGGTTGGAAGAAAGGT
    AAAGACAGCGATGTTCGTCAAAAAGATGGTAAAAACCTTGAAATGGCAATGTACTATGAC
    AAAGGTTCTTCAAGTCAAAAAGAACAAGCAGAATACTTACAAGCAGAATTTAAGAAAATG
    GGTATTAAGTTAAACATCAATGGCGAAACATCAGATAAAATTGCTGAACGTCGTACTTCT
    GGTGATTATGACTTAATGTTCAACCAAACTTGGGGATTATTGTACGATCCACAAAGTACT
    ATTGCAGCATTTAAAGCGAAAAATGGTTATGAAAGTGCAACATCAGGCATTGAGAACAAA
    GATAAAATATACAACAGCATTGATGACGCATTTAAAATCCAAAACGGTAAAGAGCGTTCA
    GACGCTTATAAAAACATTTTGAAACAAATTGATGATGAAGGTATCTTTATCCCTATTTCA
    CACGGTAGTATGACAGTTGTTGCACCAAAAGATTTAGAAAAAGTATCATTCACACAATCA
    CAGTATGAATTACCATTCAATGAAATGCAGTATAAATAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS13625 [old locus tag: NWMN_2364 ]
  • symbol: NWMN_RS13625
  • description: nickel ABC transporter, nickel/metallophore periplasmic binding protein
  • length: 532
  • theoretical pI: 9.23365
  • theoretical MW: 60018.7
  • GRAVY: -0.809962

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 787.6)
    MetabolismTransport and binding proteinsUnknown substrateABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 115.2)
  • TheSEED:
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 266.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.392
    • Ymax_pos: 27
    • Cmax: 0.248
    • Cmax_pos: 27
    • Smax: 0.929
    • Smax_pos: 3
    • Smean: 0.739
    • D: 0.527
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447151821 NCBI
  • RefSeq: WP_001229077 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS13625 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]