From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2476 [new locus tag: SACOL_RS12980 ]
  • pan locus tag?: SAUPAN006027000
  • symbol: SACOL2476
  • pan gene symbol?: cntA
  • synonym:
  • product: peptide ABC transporter peptide-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2476 [new locus tag: SACOL_RS12980 ]
  • symbol: SACOL2476
  • product: peptide ABC transporter peptide-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2532192..2533790
  • length: 1599
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAGAAAACTAACTAAAATGAGTGCAATGTTACTTACATCAGGGCTAATTTTAACTGGT
    TGTGGCGGTAATAAAGGTTTAGAGGAGAAAAAAGAAAACAAGCAATTAACGTATACGACG
    GTTAAAGATATCGGTGATATGAATCCGCATGTTTACGGTGGATCAATGTCTGCTGAAAGT
    ATGATATACGAGCCGCTTGTACGTAACACGAAAGATGGTATTAAGCCTTTACTAGCTAAA
    AAGTGGGATGTGTCTGAAGATGGGAAGACATACACGTTCCATTTGAGAGATGACGTTAAA
    TTCCATGATGGTACGCCATTTGATGCTGACGCAGTTAAGAAAAATATTGACGCAGTTCAA
    GAAAACAAAAAATTGCATTCTTGGTTAAAGATTTCGACATTAATTGACAATGTTAAAGTT
    AAAGATAAGTACACGGTTGAATTGAATTTGAAAGAAGCATATCAACCTGCATTGGCTGAA
    TTAGCGATGCCTCGTCCATATGTATTTGTGTCTCCAAAAGACTTTAAAAACGGTACAACA
    AAAGATGGCGTTAAAAAGTTCGATGGTACTGGTCCATTTAAATTAGGTGAACACAAAAAA
    GATGAGTCTGCAGACTTTAACAAAAATGATCAATACTGGGGCGAAAAGTCTAAACTTAAC
    AAAGTACAAGCAAAAGTAATGCCTGCTGGTGAAACAGCATTCCTATCAATGAAAAAAGGT
    GAAACGAACTTTGCCTTCACAGATGATAGAGGTACAGATAGCTTAGACAAAGACTCTTTA
    AAACAATTGAAAGATACAGGTGACTATCAAGTTAAGCGTAGTCAACCTATGAATACGAAA
    ATGTTAGTTGTCAATTCTGGTAAAAAAGATAACGCTGTGAGTGACAAAACAGTCAGACAA
    GCGATTGGTCATATGGTAAACAGAGATAAAATTGCCAAAGAAATTTTAGATGGTCAAGAA
    AAACCAGCAACTCAATTATTTGCGAAAAATGTAACAGACATTAATTTCGATATGCCAACA
    CGTAAGTATGACCTTAAAAAAGCAGAATCATTATTAGATGAAGCTGGTTGGAAGAAAGGT
    AAAGACAGCGATGTTCGTCAAAAAGATGGTAAAAACCTTGAAATGGCAATGTACTATGAC
    AAAGGTTCTTCAAGTCAAAAAGAACAAGCAGAATACTTACAAGCAGAATTTAAGAAAATG
    GGTATTAAGTTAAACATCAATGGCGAAACATCAGATAAAATTGCTGAACGTCGTACTTCT
    GGTGATTATGACTTAATGTTCAACCAAACTTGGGGATTATTGTACGATCCACAAAGTACT
    ATTGCAGCATTTAAAGCGAAAAATGGTTATGAAAGTGCAACATCAGGCATTGAGAACAAA
    GATAAAATATACAACAGCATTGATGACGCATTTAAAATCCAAAACGGTAAAGAGCGTTCA
    GACGCTTATAAAAACATTTTGAAACAAATTGATGATGAAGGTATCTTTATCCCTATTTCA
    CACGGTAGTATGACAGTTGTTGCACCAAAAGATTTAGAAAAAGTATCATTCACACAATCA
    CAGTATGAATTACCATTCAATGAAATGCAGTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1599

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2476 [new locus tag: SACOL_RS12980 ]
  • symbol: SACOL2476
  • description: peptide ABC transporter peptide-binding protein
  • length: 532
  • theoretical pI: 9.23365
  • theoretical MW: 60048.7
  • GRAVY: -0.814662

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 787.6)
    MetabolismTransport and binding proteinsUnknown substrateABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 115.2)
  • TheSEED:  
    Membrane TransportMembrane Transport - no subcategoryTransport of Nickel and Cobalt Nickel ABC transporter, periplasmic nickel-binding protein NikA (TC 3.A.1.5.3) 
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 266.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LILTGCG
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.401
    • Ymax_pos: 27
    • Cmax: 0.265
    • Cmax_pos: 27
    • Smax: 0.921
    • Smax_pos: 3
    • Smean: 0.726
    • D: 0.528
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRKLTKMSAMLLTSGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Laetitia Remy, Marie Carrière, Aurélie Derré-Bobillot, Cécilia Martini, Maurizio Sanguinetti, Elise Borezée-Durant
The Staphylococcus aureus Opp1 ABC transporter imports nickel and cobalt in zinc-depleted conditions and contributes to virulence.
Mol. Microbiol.: 2013, 87(4);730-43
[PubMed:23279021] [WorldCat.org] [DOI] (I p)