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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
  • pan locus tag?: SAUPAN006022000
  • symbol: SACOL2471
  • pan gene symbol?:
  • synonym:
  • product: transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
  • symbol: SACOL2471
  • product: transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2527619..2528812
  • length: 1194
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAGGTGCAATGGCTTGGCCCTTTTTGAGATTATATATATTAACATTGATGTTCTTT
    AGTGCCAATGCAATCTTAAACGTGTTTATACCTTTACGAGGGCATGATTTAGGCGCAACG
    AATACGGTTATCGGTATCGTTATGGGGGCATACATGTTAACAGCAATGGTATTTCGACCA
    TGGGCAGGACAAATTATTGCTCGTGTCGGTCCCATTAAAGTATTAAGATTTATTTTGATT
    ATCAATGCCATAGCTTTAATTATTTATGGTTTTACTGGCTTAGAAGGTTATTTCGTAGCA
    CGTGTTATGCAAGGTGTGTGTACGGCATTCTTTTCTATGTCTTTACAGCTAGGTATTATT
    GATGCATTACCAGAGGAACATCGTTCTGAAGGTGTATCATTGTACTCGCTATTTTCAACG
    ATTCCAAACTTAATCGGACCATTAGTTGCCGTAGGTATTTGGAATGCAAATAATATTTCA
    CTATTTGCAATTGTCATTATCTTTATCGCATTAACAACAACATTCTTTGGCTATCGCGTG
    ACCTTTGCTGAACAGGAACCCGATACGTCAGATAAGATTGAAAAAATGCCGTTTAACGCT
    GTAACTGTTTTTGCGCAATTTTTCAAAAATAAAGAGTTGTTGAACAGTGGTATTATCATG
    ATTGTTGCATCGATTGTATTTGGTGCAGTTAGTACATTTGTACCGTTATACACAGTGAGT
    TTAGGATTCGCGAATGCGGGAATCTTTTTGACAATACAGGCCATCGCAGTTGTTGCGGCA
    AGATTTTACTTAAGGAAATACATTCCGTCAGATGGTATGTGGCATCCTAAATATATGGTA
    TCTGTACTATCATTATTAGTAATCGCGTCATTTGTAGTGGCATTTGGTCCGCAAGTAGGT
    GCAATTATTTTCTATGGTAGTGCGATATTAATAGGAATGACGCAAGCAATGGTGTACCCA
    ACATTAACATCATACTTAAGCTTCGTCTTACCAAAAGTAGGTCGTAATATGTTGTTAGGT
    TTATTTATTGCCTGTGCAGACTTAGGTATATCGTTAGGTGGCGCATTGATGGGACCTATT
    TCCGATTTAGTAGGATTTAAATGGATGTATCTAATTTGTGGTATGTTAGTCATTGTAATA
    ATGATTATGAGTTTCTTGAAAAAGCCAACACCACGTCCAGCGAGTAGTCTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
  • symbol: SACOL2471
  • description: transporter
  • length: 397
  • theoretical pI: 9.73958
  • theoretical MW: 43328.9
  • GRAVY: 0.975567

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 44.6)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsH+ Antiporter protein (TIGR00900; HMM-score: 35.3)
    multidrug resistance protein (TIGR00880; HMM-score: 33.7)
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 21.6)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 21.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 6.5)
  • TheSEED:  
    Antibiotic resistance protein 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 135.4)
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 28.1)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.175
    • Ymax_pos: 13
    • Cmax: 0.118
    • Cmax_pos: 40
    • Smax: 0.378
    • Smax_pos: 7
    • Smean: 0.308
    • D: 0.227
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVIGIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRFILIINAIALIIYGFTGLEGYFVARVMQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNISLFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGIIMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHPKYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVGRNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPRPASSL

Experimental data[edit source | edit]

  • experimental localization: Integral membrane [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]