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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS13740 [old locus tag: NWMN_2386 ]
  • pan locus tag?: SAUPAN006083000
  • symbol: NWMN_RS13740
  • pan gene symbol?:
  • synonym:
  • product: helicase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS13740 [old locus tag: NWMN_2386 ]
  • symbol: NWMN_RS13740
  • product: helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 2619759..2622620
  • length: 2862
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2619759..2622620) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTAGATTACTAAATGATTTCAATCAATCTTTACATAAAGGGTTTATAGACAAACAT
    ATTTCTCATAAAGGGAATTATACACCGAAGCTTTTAGTAAATAATAAAAATGAAAAAGTT
    CTTTCAACAATTATAGATGAACTTCAAAAATGTGAAACGTTTTATTTTTCTGTGGCTTTT
    ATAACAGAAAGCGGTTTAGCCAGCTTAAAGGCTCAACTATTAGATTTAAGCAATAAGGGT
    GTTAAAGGGAAAATATTAACATCTAATTACTTAGGGTTTAATAGTCCTAAAATGTATGGA
    GAATTACTTAAATTAAAAAATGTAGAGGTAAGATTAACTGATATTGCTGGATTCCATGCC
    AAAGGATATATTTTTGAGCATAAGGATTATAGTTCTATGGTAATAGGAAGCTCTAATTTA
    ACATCTAATGCGTTAAAGGTTAATTATGAGCATAATGTTTTACTGTCTACTATGAAAAAT
    GGCGATTTAGTTGATAGTGTAAAAAGTGAATTTGACTTGTTATGGCAAAAGAGTACCCCA
    CTGACTGAGCAATGGATTAATTCATATAAAGAATCATTTGAGTACCGTTCCTTAGAAAAA
    TTAGCGGAGGTTGAACAAACTCAAATGCTGCTAGCTGATAAGGTGAAAAAATCAGTGGAA
    ATTGTACCCAATTTAATGCAAGCAGAAGCATTAAGGTCATTAAAAGCTATTAGGGATAAA
    GCCAAAGATAAGGCGTTAATAATATCTGCAACTGGTACAGGTAAAACGATTTTATGTGCA
    TTAGATGTTAGAGAAGTAAACCCTAATAAATTTTTATTTATTGTTCATAATGAGGGGATT
    TTAAATAGAGCTAAGGAAGAATTTAAAAAAGTATTACCTATAAAAAATGATAGTGATTTT
    GGATTGTTGACAGGAAAACATAGAGATGTCGATGCCAAATATTTATTTGCAACCATTCAA
    ACATTATCTAGAGATGATAATTTTAAGCAATTTGATGAAAATGAATTTGATTATATTGTT
    TTTGATGAAGCACATCGTTCTGCTGCATCTACTTATCAACGAGTATTTAACTACTTCAAA
    CCTAAGTTCATGCTGGGAATGACTGCTACACCAGAAAGATCAGATGAATTAAGTATATTT
    GAACTGTTTGATTATAATATTGCATATGAAATAAGGTTACAAGCAGCATTAGAAAGTGAT
    ATTTTATGTCCATTTCATTATTTTGGTGTGACTGATTATGTACATCAAGGTATTAAAGAA
    GATGATGTAACTAAACTGAGATATTTAACTTCTGATGAAAGAGTTAATTATATTATTCAA
    AAGACAGATTACTATGGATATTCAGGTGAAATTTTACAAGGATTGATTTTTGTAAGTAGC
    AAAAAAGAAGCTTATGATTTAGCGGATAAACTAAGTTCAAAAGGGATTAAAAGTGTTGCT
    TTAACAGGAGATGATAGTGTTAATTATCGCCAAATTGTAATAGAAAAATTAAAAGAAGGT
    AAGATTAACTATATTATAACTGTAGATCTTTTTAACGAAGGTATTGATATTCCAGAAGTC
    AATCAAGTTGTAATGTTGAGACCAACAGAGTCTAGTATTATATTCATTCAGCAACTTGGT
    AGAGGATTAAGAAAAAGTTCAAATAAAGAGTATGTTACAGTAATTGATTTTATTGGTAAT
    TATAAGACAAATTATTTAATTCCGATTGCGCTTTCTGGGGATCAATCGCAAAATAAAGAT
    AATTATAAGAAGTTTTTAACGAATAACGATTCGATTAATGGAGTATCTACAATTAATTTT
    GAAGAAGTTGCTAAAAAACAGATTTACAATTCATTGGATGCAGTATCATTAAATCAAAAT
    AAATTAATATTAAAAGCTTATGAAGAAGTTGAAAATAGATTGGGACACATGCCGTTACTA
    ATGGATTTCATACAACAACATTCTATAGATCCAAGCGTTATATTTTCTAAATTTAGTAAT
    TATTACGAGTTCTTAGTGAGATATAAAAAAATAGATACATTATTGACGGAAAATGAATCT
    AAAAATCTGGTTTTCTTTTCAAGGCAAATAGCACCTGGATTGAAAAGAATCGATAGTTTA
    GTTTTAGAAGAACTTTTAAAAAATGAATTAACATATGATGAATTAAAAAATAAAATGTTG
    AACGAAGTTAAGGATATAACAGAAGATGATATAGATACTTCATTAAGAATTTTAGATTTT
    TCATTTTACAACGCAGGAATTGAAAAAATCTATGGAAGTCCCATTATAGAACGTAATGAA
    AGAATGATAAGACTAAGCGATGCATTTACTAATGCTCTAAGTAATCAAACATTTAATATG
    TTTTTAGAAGATCTTATTGAATTATCAAAGTATAACAATGAAAAATACCAAAAAGGTAAA
    AATGGACTGATTTTATATAATAAATATTCAAGAGAAGATTTTAGTAAAATATTTAATTGG
    AATAAAAATGGTTCGTCTGTAATCATGGGATACATGATAAAAAGCCAAGAAATGCCAATA
    TTTATCACTTATGATAAGCATGAGGATATAAGTGATAGCACAAAATATGAAGATGAATTT
    TTAAGTCAAGATGAATTAAAATGGTTTACTAAATCTAATAGAACGCTAGAATCAAAAGAA
    GTTCAGAAAATTTTGTCCCATAGAGCAAAAGGAATTAAAATGTATATTTTTGTACAGAAA
    AAAGATGATGATGGTATATATTTTTATTATTTAGGAACTGCTGGATATATAGAAGGTTCA
    GAAAAGCAAGATAAAATGCCGAATGGTTCAAACGTAGTAACGATGGATTTAGCACTGGAT
    AAAGCTGTAAGAGATGATATATATCGTTATCTAACAAATTAA
    60
    120
    180
    240
    300
    360
    420
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    720
    780
    840
    900
    960
    1020
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    1140
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    1260
    1320
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    1560
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    1680
    1740
    1800
    1860
    1920
    1980
    2040
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    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2862

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS13740 [old locus tag: NWMN_2386 ]
  • symbol: NWMN_RS13740
  • description: helicase
  • length: 953
  • theoretical pI: 6.08051
  • theoretical MW: 109900
  • GRAVY: -0.407765

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 70.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 53.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 24.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 22.1)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 14)
  • TheSEED:
  • PFAM:
    no clan definedDUF3427; Domain of unknown function (DUF3427) (PF11907; HMM-score: 268.6)
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 120.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 61.7)
    PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 56.4)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 26.5)
    DUF2075; Uncharacterized conserved protein (DUF2075) (PF09848; HMM-score: 20.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.14
    • Ymax_pos: 22
    • Cmax: 0.104
    • Cmax_pos: 41
    • Smax: 0.229
    • Smax_pos: 10
    • Smean: 0.172
    • D: 0.152
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446010867 NCBI
  • RefSeq: WP_000088722 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS13740 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]