From AureoWiki
Jump to: navigation, search
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14795 [old locus tag: NWMN_2579 ]
  • pan locus tag?: SAUPAN006437000
  • symbol: NWMN_RS14795
  • pan gene symbol?:
  • synonym:
  • product: polysaccharide deacetylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS14795 [old locus tag: NWMN_2579 ]
  • symbol: NWMN_RS14795
  • product: polysaccharide deacetylase
  • replicon: chromosome
  • strand: -
  • coordinates: 2846938..2848062
  • length: 1125
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2846938..2848062) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCATAAAAAATATTTATTGCTATTTTTTCTGTTATTATCAGTTGCATTGTTATATAGC
    TGTTCCAAGCAAAATGAGAAAGCTCAAAATAAATCTGTTAATCCAAATGAGTATGCAAAG
    CATAAATCGAAAATGGATGAACATTGGGAAACATATCACGGCCAAGTTTATCATGTGTTT
    TATCACCCATTAATTACAGACCCAAAAGTTGCGTTTACAGGAGATACACACCAAGCTAAG
    GGCAACAATGATTGGATGATTACTGTAAGTGAATTTAAAAAATCATTGAACGAATTATAT
    AAAAATAATTACATCATCGTTAATCCACATGATGTATACGACTTATCATCTCATCATGTA
    AAAGCCAAAAAAATAAAATTGCCGAAAGGTAAAAAGCCACTAATCATCTCAATTGATGAT
    ATGAATTACTATAGTTACATGAGACACCATGGTTATGCTGACCGTTTAGTACTCGATAAA
    AATAAACATGTTGTTTCTGAAACTACAGACAAAAAAGGCCATGCTACTTATTCTGATGAC
    AATGATATCGTGCCAATTTTAAATAAATTTGTAAAAGAGCATCCAGATTTTTCTTTAAAT
    GGTGAAAAAGGTGTTGTTGCTTTAACCGGTTATGAGGGCGTATTAGGTTATCGCACGAAT
    GAATTAACGAGTAAAGATTATACAAAGAATAAAAAGGCAGCTGAGGAAGTTGTTCGCGCA
    ATGAAACGGGATGGATGGTCATTTGCGAGTCATTCATATGGTCACATTAATTTTGAAAAA
    ACATCTTTAGAAGGTATTAAACGTGATACTAAAAGATGGAAAGATGAAGTTGAACCAATT
    GTAGGTAAAACAGATATGTTTGTATTTCCACATGGCGCACAGGATAGACATACACAAGCG
    TATGATTATTTGGTAGATGAAGCAGAGTTTAAGTTTATTGCAGGTGTCGGTCCAAACAAT
    TTTACAGATATATCAGCAACGAACGTTTACCAAGATAGGGTTGCGATTGATGGTCTAAAC
    TTATTTGAATTTAAATATAAACTCAAACCGTTTTTTAATCCTGAAAATGTATATAGTAAA
    CAAGATAGACGTTACTTTAAAGGGAATCGGGATTATGAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS14795 [old locus tag: NWMN_2579 ]
  • symbol: NWMN_RS14795
  • description: polysaccharide deacetylase
  • length: 374
  • theoretical pI: 9.01084
  • theoretical MW: 43552.8
  • GRAVY: -0.757754

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationpolysaccharide deacetylase family sporulation protein PdaB (TIGR02764; HMM-score: 24.9)
    aromatic cluster surface protein (TIGR04313; HMM-score: 23.3)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycandelta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
    Cellular processesCellular processesSporulation and germinationdelta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidespolysaccharide deacetylase family protein, PEP-CTERM locus subfamily (TIGR03006; HMM-score: 16.4)
    putative urate catabolism protein (TIGR03212; HMM-score: 12.9)
  • TheSEED:
  • PFAM:
    GH_CE (CL0158) Polysacc_deac_1; Polysaccharide deacetylase (PF01522; HMM-score: 31.8)
    no clan definedETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 15.1)
    DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 15)
    HicA_toxin; HicA toxin of bacterial toxin-antitoxin, (PF07927; HMM-score: 12.7)
    GH_CE (CL0158) DUF2334; Uncharacterized protein conserved in bacteria (DUF2334) (PF10096; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.87
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.635
    • Ymax_pos: 29
    • Cmax: 0.505
    • Cmax_pos: 29
    • Smax: 0.903
    • Smax_pos: 22
    • Smean: 0.788
    • D: 0.694
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446471369 NCBI
  • RefSeq: WP_000549223 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSKMDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKNNYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDKNKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYRTNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDEVEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRVAIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS14795 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]