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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03016
  • pan locus tag?: SAUPAN006437000
  • symbol: SAOUHSC_03016
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_03016
  • symbol: SAOUHSC_03016
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2789402..2790526
  • length: 1125
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCATAAAAAATATTTATTGCTATTTTTTCTGTTATTATCAGTTGCATTGTTATATAGC
    TGTTCCAAGCAAAATGAGAAAGCTCAAAATAAATCTGTTAATCCAAATGAGTATGCAAAG
    CATAAATCGAAAATGGATGAACATTGGGAAACATATCACGGCCAAGTTTATCATGTGTTT
    TATCACCCATTAATTACAGACCCAAAAGTTGCGTTTACAGGAGATACACACCAAGCTAAG
    GGCAACAATGATTGGATGATTACTGTAAGTGAATTTAAAAAATCATTGAACGAATTATAT
    AAAAATAATTACATCATCGTTAATCCACATGATGTATACGACTTATCATCTCATCATGTA
    AAAGCCAAAAAAATAAAATTGCCGAAAGGTAAAAAGCCACTAATCATCTCAATTGATGAT
    ATGAATTACTATAGTTACATGAGACACCATGGTTATGCTGACCGTTTAGTACTCGATAAA
    AATAAACATGTTGTTTCTGAAACTACAGACAAAAAAGGCCATGCTACTTATTCTGATGAC
    AATGATATCGTGCCAATTTTAAATAAATTTGTAAAAGAGCATCCAGATTTTTCTTTAAAT
    GGTGAAAAAGGTGTTGTTGCTTTAACCGGTTATGAGGGCGTATTAGGTTATCGCACGAAT
    GAATTAACGAGTAAAGATTATACAAAGAATAAAAAGGCAGCTGAGGAAGTTGTTCGCGCA
    ATGAAACGGGATGGATGGTCATTTGCGAGTCATTCATATGGTCACATTAATTTTGAAAAA
    ACATCTTTAGAAGGTATTAAACGTGATACTAAAAGATGGAAAGATGAAGTTGAACCAATT
    GTAGGTAAAACAGATATGTTTGTATTTCCACATGGCGCACAGGATAGACATACACAAGCG
    TATGATTATTTGGTAGATGAAGCAGAGTTTAAGTTTATTGCAGGTGTCGGTCCAAACAAT
    TTTACAGATATATCAGCAACGAACGTTTACCAAGATAGGGTTGCGATTGATGGTCTAAAC
    TTATTTGAATTTAAATATAAACTCAAACCGTTTTTTAATCCTGAAAATGTATATAGTAAA
    CAAGATAGACGTTACTTTAAAGGGAATCGGGATTATGAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_03016
  • symbol: SAOUHSC_03016
  • description: hypothetical protein
  • length: 374
  • theoretical pI: 9.01084
  • theoretical MW: 43552.8
  • GRAVY: -0.757754

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationpolysaccharide deacetylase family sporulation protein PdaB (TIGR02764; HMM-score: 24.9)
    aromatic cluster surface protein (TIGR04313; HMM-score: 23.3)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycandelta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
    Cellular processesCellular processesSporulation and germinationdelta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidespolysaccharide deacetylase family protein, PEP-CTERM locus subfamily (TIGR03006; HMM-score: 16.4)
    putative urate catabolism protein (TIGR03212; HMM-score: 12.9)
  • TheSEED:  
    FIG01107888: hypothetical protein 
  • PFAM:
    GH_CE (CL0158) Polysacc_deac_1; Polysaccharide deacetylase (PF01522; HMM-score: 31.8)
    no clan definedETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 15.1)
    DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 15)
    HicA_toxin; HicA toxin of bacterial toxin-antitoxin, (PF07927; HMM-score: 12.7)
    GH_CE (CL0158) DUF2334; Uncharacterized protein conserved in bacteria (DUF2334) (PF10096; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.87
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: ALLYSCS
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.635
    • Ymax_pos: 29
    • Cmax: 0.505
    • Cmax_pos: 29
    • Smax: 0.903
    • Smax_pos: 22
    • Smean: 0.788
    • D: 0.694
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSKMDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKNNYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDKNKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYRTNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDEVEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRVAIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]