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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0226 [new locus tag: SA_RS01325 ]
  • pan locus tag?: SAUPAN001095000
  • symbol: SA0226
  • pan gene symbol?: fadE
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0226 [new locus tag: SA_RS01325 ]
  • symbol: SA0226
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 272223..273728
  • length: 1506
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAATTTCGATTGGATAAAAACGCGTTCAGACTTCGATGATGACAAGCCTGCCGTTATT
    GATCACGCAAAACAAACATCTTGGACATACCAACAACTCAATGCACGCGCTGATAATATG
    GCACATTATTTAACATCTCAAGGTGTTAAAAAAGGCGATGTTATCGGTATTTTTGCGCCA
    AATGATATTGCAATATTAGATTTATTGTTTGCTTGTTTTAAAACAGGTGCGGTTTTTTTA
    CCATTGAATTGGCGGCTTAACCCAAAAGAAATTGCAGCCATTGTGGAAGATGCACAATTA
    AAACTGCTCTTCTATGCTGAAAAACATTTAAGTTCACTCACCGATATTGACCAAAACTTA
    TTGCATATGGATATTGATGTGGCGCAATATGATGAAATCGTAAATCCAGATTATCACCAA
    CCTTTTCAAGCAACACCTGTTGAACCACAAGATCTTGCAGCATTAATTTATACAAGTGGT
    ACAACCGGATCACCTAAAGGTGTGATGTTCTCATATGAATCATTTGTTCATAACGGTGCA
    AACTTAGAGCTGACGTACAAGTTCAATTCAAACTATATTACGATTGTATCAACACCAATG
    TTCCATGTTTTAGGGTTTAACGATACTGTATTACCAGTATTAATGTCAGGTGGCACACTT
    ATTCTTCAACGTTACTTTAACGGCGAAGAATTGAATGACATGATTGCACAATATCACCCT
    ACATTTATTATCATGATTCCGACAATGTATTATAGTACGCTACGTGCTAGTAATTTTAAT
    CCTGAAAATTTTAGAGCTATGGATTATATCATCCAAGGTGGTTCACAACCATTACCAAGT
    ATCCAAGCAGCTTTTAAACAATATGGCATAAACATTATTAACGGCTATGGTTTAACTGAA
    GCACCTCTTGTACTTGTTAATACACCGGAAAATTCAAAACGTAAGCCAATGAGTATTGGT
    AAAGCAGTCATGTTCGTTGATGCACGTATCCTTGATGATAACGGTGAGGAAGTACCTACT
    GGTGAGATTGGCGAACTTGCAATTAAGGCTAAAAATGTCACGCCAGGATATTGGAATAAA
    CCAGCAGAGACTGCCAAAGCATTTCATGGTCGTTATTTATTAACTGGTGACTTAGCGAAG
    ATGGACGACGATGGCGATATATTTATTATTGACCGCAAAAAAGAATTAATCATAACTGGT
    GGCGAAAATGTCTTACCATCTGAAGTCGAAAATGCTTTAGCTGAGCATCCACTAGTAGAC
    CGGTGTGTGGTCGTTGGCTATGATCATCCAAAATATGGTGAATCAATTGCTGCAGCCATT
    ATACTTCGCGAAGATGAACCTCATTACGCTGAAATTTTAGATCAACATATGCGAAGTCGT
    TTAGCAGGTTATAAAGTCCCAAGAATGTATGTACCAGTGACACATATGCCGTTAAACAGT
    ACGCAGAAACCAGATAAACTTGCGATTCGACAAATGATGAATGACAAAGTCTCACAAACA
    CTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1506

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0226 [new locus tag: SA_RS01325 ]
  • symbol: SA0226
  • description: hypothetical protein
  • length: 501
  • theoretical pI: 5.01311
  • theoretical MW: 56206.8
  • GRAVY: -0.198802

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneO-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 271.4)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 249.5)
    amino acid adenylation domain (TIGR01733; HMM-score: 227.4)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 193.4)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 180.6)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 165.2)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 152.8)
    MetabolismTransport and binding proteinsCations and iron carrying compounds(2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 143.1)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 122.6)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 69.2)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 53.5)
    MetabolismCentral intermediary metabolismOtheracetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 34.6)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 27.9)
    MetabolismEnergy metabolismOtherphenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 11.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsBiotinBiotin biosynthesis Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) 
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 331.4)
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 52.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 38
    • Cmax: 0.167
    • Cmax_pos: 38
    • Smax: 0.125
    • Smax_pos: 34
    • Smean: 0.056
    • D: 0.09
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYLTSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKLLFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSGTTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGGTLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQPLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDNGEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDRKKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYAEILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]