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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0617 [new locus tag: SA_RS03540 ]
  • pan locus tag?: SAUPAN002541000
  • symbol: vraG
  • pan gene symbol?: vraG
  • synonym:
  • product: ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0617 [new locus tag: SA_RS03540 ]
  • symbol: vraG
  • product: ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 710835..712736
  • length: 1902
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    GTGGCGTTAATTATGACCTTTAACGAGATAATATTTAAAAATTTCCGTCAAAATTTATCA
    CATTATGCCATCTATCTTTTTTCATTAATTACGAGTGTAGTATTGTATTTTAGCTTTGTA
    GCATTAAAATACGCGCATAAACTAAACATGACAGAGTCATATCCAATTATTAAGGAAGGC
    TCACAAGTCGGAAGCTACTTTCTATTTTTCATCATAATTGCATTTTTGTTATATGCCAAT
    GTGTTATTTATTAAACGACGAAGTTATGAGCTTGCATTATATCAAACATTAGGTTTATCT
    AAATTCAACATTATTTATATACTAATGCTCGAACAATTACTAATATTTATAATTACGGCA
    ATATTAGGTATTATTATTGGTATTTTTGGTTCAAAACTGTTATTAATGATTGTCTTTACA
    TTATTAGGAATTAAAGAAAAGGTTCCAATTATTTTTAGTTTGAGGGCGGTATTTGAAACA
    TTAATGTTAATCGGTGTCGCTTATTTTTTAACCTCTGCTCAAAATTTTATATTAGTGTTC
    AAACAATCTATTTCACAGATGTCAAAGAATAACCAGGTTAAAGAAACAAATCATAATAAA
    ATTACATTTGAAGAGGTTGTTTTAGGCATCTTAGGTATAGTATTGATTACCACAGGATAC
    TATCTATCTTTGAACATTGTTCAATATTATGATTCTATCGGTATACTTATGTTTATTTTA
    TTGTCAACTGTGATTGGGGCATACTTATTTTTTAAAAGCTCTGTTTCTCTAGTTTTTAAA
    ATGGTGAAGAAGTTTAGAAAAGGTGTTATAAGTGTAAATGATGTCATGTTCTCATCATCT
    ATTATGTATCGTATTAAGAAAAATGCTTTTTCACTTACGGTCATGGCAATCATTTCAGCG
    ATTACTGTTTCAGTTCTTTGCTTTGCTGCTATAAGTAGAGCGTCCTTATCAAGTGAAATA
    AAATATACTGCACCACACGACGTTACAATTAAAGACCAACAAAAAGCTAATCAATTAGCA
    AGTGAATTAAACAATCAAAAAATTCCTCATTTTTATAATTATAAAGAAGTAATTCATACG
    AAATTGTATAAAGATAATTTATTTGATGTAAAAGCGAAAGAACCATACAATGTAACAATT
    ACTAGTGATAAATATATCCCTAATACTGATTTGAAACGTGGACAAGCTGATTTGTTTGTA
    GCGGAAGGTTCTATCAAAGATTTAGTGAAACATAAGAAGCATGGTAAGGCAATTATAGGA
    ACGAAAAAACATCATGTTAATATTAAGTTACGGAAAGATATTAATAAAATCTATTTTATG
    ACAGATGTTGATTTAGGTGGACCAACGTTTGTCTTAAATGACAAAGACTATCAAGAAATA
    AGAAAGTATACAAAAGCAAAGCATATCGTCTCTCAATTTGGATTCGATTTGAAACATAAA
    AAAGATGCTTTAGCATTAGAAAAAGTGAAAAATAAAGTTGATAAATCTATTAAAACAAGA
    AGTGAAGCGATAAGCTCAATATCAAGTTTAACCGGAATATTATTATTTGTAACATCATTT
    TTAGGTATTACATTCTTGATTGCTGTATGTTGCATTATATACATTAAGCAAATAGATGAA
    ACCGAAGATGAGTTAGAGAATTATAGTATATTGAGAAAGCTTGGATTTACACAAAAAGAT
    ATGGCAAGGGGACTAAAGTTTAAAATTATGTTTAATTTTGGGTTACCTTTAGTTATTGCA
    CTATCACATGCATATTTTACATCATTAGCATATATGAAATTAATGGGTACAACGAATCAA
    ATACCGGTTTTCATAGTAATGGGATTATACATTTGTATGTATGCTGTTTTTGCAGTGACG
    GCTTATAATCATTCCAAGCGAACAATTAGACATTCCATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1902

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0617 [new locus tag: SA_RS03540 ]
  • symbol: VraG
  • description: ABC transporter permease
  • length: 633
  • theoretical pI: 10.1152
  • theoretical MW: 72199.3
  • GRAVY: 0.383096

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 13.8)
  • TheSEED:  
    Virulence, Disease and DefenseBacteriocins, ribosomally synthesized antibacterial peptidesBacitracin Stress Response Bacitracin export permease protein BceB 
  • PFAM:
    BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 22.9)
    no clan definedTMEM251; Transmembrane protein 251 (PF15190; HMM-score: 8.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.133
    • Ymax_pos: 44
    • Cmax: 0.148
    • Cmax_pos: 41
    • Smax: 0.29
    • Smax_pos: 43
    • Smean: 0.099
    • D: 0.12
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

  • GI: 15926339 NCBI
  • RefSeq: NP_373872 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MALIMTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKLNMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIYILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLIGVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYLSLNIVQYYDSIGILMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSSIMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQLASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQADLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLNDKDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKVKNKVDKSIKTRSEAISSISSLTGILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIMFNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRTIRHSI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]