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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00668
  • pan locus tag?: SAUPAN002541000
  • symbol: SAOUHSC_00668
  • pan gene symbol?: vraG
  • synonym:
  • product: ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00668
  • symbol: SAOUHSC_00668
  • product: ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 654690..656579
  • length: 1890
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGACCTTTAACGAGATAATATTTAAAAATTTCCGTCAAAATTTATCACATTATGCCATC
    TATCTTTTTTCGTTAATTACGAGTGTAGTATTGTATTTTAGCTTTGTAGCATTAAAATAC
    GCTCATAAACTAAACATGACAGAGTCATATCCAATTATAAAGGAAGGCTCACAAGTCGGA
    AGCTACTTTCTATTTTTCATCATAATTGCATTTTTGTTATATGCCAATGTGTTATTTATT
    AAACGACGAAGTTATGAGCTTGCATTATATCAAACATTAGGTTTATCTAAATTCAACATT
    ATTTATATACTAATGCTCGAACAATTACTAATATTTATAATTACGGCAATATTAGGTATT
    ATTATTGGTATTTTTGGTTCGAAACTGTTATTAATGATTGTCTTTACATTATTAGGAATT
    AAAGAAAAGGTTCCAATTATTTTTAGTTTGAGGGCGGTATTTGAAACATTAATGTTAATC
    GGTGTCGCTTATTTTTTAACATCTGCTCAAAATTTTATATTAGTGTTCAAACAATCTATT
    TCACAGATGTCAAAGAATAACCAGGTTAAAGAAACAAATCATAATAAAATTACATTTGAA
    GAGGTTGTTTTAGGCATCTTAGGTATAGTATTGATTACCACAGGATACTATCTATCTTTG
    AACATTGTTCAATATTATGATTCTATCGGTACACTTATGTTTATTTTATTGTCAACTGTG
    ATTGGGGCATACTTATTTTTTAAAAGCTCTGTTTCTCTAGTTTTTAAAATGGTGAAGAAG
    TTTAGAAAAGGTGTTATAAGTGTAAATGATGTCATGTTCTCATCATCTATTATGTATCGT
    ATTAAGAAAAATGCTTTTTCACTTACGGTCATGGCAATCATTTCAGCGATTACTGTTTCA
    GTTCTTTGCTTTGCTGCTATAAGTAGAGCGTCCTTATCAAGTGAAATAAAATATACTGCA
    CCACACGACGTTACAATTAAAGACCAACAAAAAGCTAATCAATTAGCAAGTGAATTAAAC
    AATCAAAAAATTCCTCATTTTTATAATTATAAAGAAGTAATTCATACGAAATTGTATAAA
    GATAATTTATTTGATGTAAAAGCGAAAGAACCATACAATGTAACAATTACTAGTGATAAA
    TACATCCCTAATACTGATTTGAAACGTGGGCAAGCTGATTTATTTGTAGCGGAAGGTTCT
    ATCAAAGATTTAGTGAAACATAAGAAGCATGGTAAGGCAATTATAGGAACGAAAAAACAT
    CATGTTAATATTAAGTTACGTAAAGATATTAATAAAATCTATTTTATGACAGATGTTGAT
    TTAGGTGGACCAACGTTTGTCTTAAATGACAAAGACTATCAAGAAATAAGAAAGTATACA
    AAGGCAAAGCATATCGTCTCTCAATTTGGATTCGATTTGAAACATAAAAAAGATGCTTTA
    GCATTAGAAAAAGCGAAAAATAAAGTTGATAAATCTATTGAAACAAGAAGTGAAGCGATA
    AGCTCAATATCAAGTTTAACCGGAATATTATTATTTGTAACATCATTTTTAGGTATTACA
    TTCTTGATTGCTGTATGTTGCATTATATACATAAAGCAAATAGATGAAACCGAAGATGAG
    TTAGAGAATTATAGTATTTTGAGAAAGCTTGGATTTACACAAAAAGATATGGCAAGGGGA
    CTAAAGTTTAAAATTATGTTTAATTTTGGGTTACCTTTAGTTATTGCACTATCACATGCA
    TATTTTACATCATTAGCATATATGAAATTAATGGGTACAACGAATCAAATACCGGTTTTC
    ATAGTAATGGGATTATACATTTGTATGTATGCTGTTTTTGCAGTGACGGCTTATAATCAT
    TCCAAGCGAACAATTAGACATTCCATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1890

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00668
  • symbol: SAOUHSC_00668
  • description: ABC transporter permease
  • length: 629
  • theoretical pI: 10.0671
  • theoretical MW: 71731.5
  • GRAVY: 0.355008

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 13.9)
  • TheSEED:  
    Virulence, Disease and DefenseBacteriocins, ribosomally synthesized antibacterial peptidesBacitracin Stress Response Bacitracin export permease protein BceB 
  • PFAM:
    BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 22.9)
    no clan definedFxsA; FxsA cytoplasmic membrane protein (PF04186; HMM-score: 6.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 40
    • Cmax: 0.154
    • Cmax_pos: 37
    • Smax: 0.305
    • Smax_pos: 39
    • Smean: 0.1
    • D: 0.125
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKLNMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIYILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLIGVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYLSLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSSIMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQLASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQADLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLNDKDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTGILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIMFNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRTIRHSI

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]