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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0645 [new locus tag: SA_RS03685 ]
  • pan locus tag?: SAUPAN002574000
  • symbol: SA0645
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0645 [new locus tag: SA_RS03685 ]
  • symbol: SA0645
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 738629..740182
  • length: 1554
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCAGAAGAAAAACATGTAGTGGAACATGAACAACAAAAGAAAGAAAAGACAAAAAAG
    CAATACAAGCCATTTTGGATTGTCATGAGTTTTATAATACTTATAGTTGTACTATTACTC
    CCGGCACCTTCAAGTCTGCCGATAATGGCTAAGGCAGTACTAGCTATTTTAGCTTTTGCA
    GTTATTATGTGGGTAACGGAAGCTGTATCATATCCGGTGTCAGCAACTTTAATTATTGGC
    TTAATGATATTACTTTTAGGATTTAGCCCGGTTCAAAATTTAGGGGAGAAGCTAGGTAAT
    CCGAAAAGTGGCAGTGCTATTTTAGCTGGAAGTGACCTTCTAGGAACTAATCATGCATTA
    TCATTAGCGTTTAGTGGATATGCAACTTCAGCTGTAGCTCTCGTTGCAGCTGCATTATTT
    TTGGCTGCTGCTATGCAAGAAACGAATTTGCATAAAAGACTAGCTCTTTTAGTGTTATCA
    ATTGTTGGTAATAAAACTAGAAATATAGTTATTGGAGCAATTATCGTTTCAATTGTACTT
    GCATTTTTCGTTCCTTCTGCAACAGCTAGAGCAGGGGCAGTTGTACCAATCTTGCTGGGT
    ATGATTGCGGCATTTAAAGTTTCCAAAGATAGCAAGTTAGCGTCTTTATTAATAATTACT
    TCAGTACAAGCTGTGTCAATTTGGAATATTGGTATCAAAACGGCGGCAGCACAAAATATC
    GTAGCGATTAATTTTATAAACCATCAATTAGGATTTGATGTTTCATGGGGCGAGTGGTTC
    TTATATGCAGCGCCTTGGTCCATAGTTATGTCCGTAGCTTTATATTTCATCATGATTAAA
    GTGATGCCTCCAGAAATTAATACAATAGAAGGTGGTAAAGATTTAATAAAAGAAGAATTG
    CATAAACTTGGCCCCGTTAGCCCACGTGAATGGCGTTTAATTGTTATATCGATGTTATTA
    TTACTGTTTTGGTCAACTGAAAAAGTATTACATCCGATTGACTCTGCATCCATTACTATT
    ATTGCTTTAGGTGTTATGTTAATGCCGAAAATTGGTGTCATGACATGGAAACATGTTGAA
    AATAAAATACCATGGGGAACAATTATCGTGTTTGGTGTAGGTATTTCACTAGGGAACGTT
    CTTTTGAAAACAGGTGCAGCTCAATGGTTAAGTGATCAAACTTTTGGTGTTTTAGGTTTA
    AAACATTTACCTATTATCGCGACAATTGCACTTATCACGCTTTTTAATATATTGATTCAT
    TTGGGCTTTGCGAGTGCAACAAGTTTATCATCAGCGTTAATACCTGTTTTTATTTCGCTA
    ACCTCTACGTTACACTTAGGAGACCAGTCTATAGGATTTGTTTTAATTCAACAATTTGTT
    ATTAGTTTTGGTTTCTTATTACCTGTTAGTGCACCTCAAAATATGTTGGCTTATGGCACT
    GGTACTTTTACGGTTAAAGATTTCTTGAAGGCAGGTATACCATTGACAATTGTAGGGTAT
    ATTCTAGTGATAGTTTTTAGTATGACTTATTGGAAATGGTTAGGTTTGCTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0645 [new locus tag: SA_RS03685 ]
  • symbol: SA0645
  • description: hypothetical protein
  • length: 517
  • theoretical pI: 10.1134
  • theoretical MW: 55879.7
  • GRAVY: 0.881238

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257)
    Metabolism Transport and binding proteins Anions transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257)
    and 1 more
    TIGR03943 family protein (TIGR03943; HMM-score: 6.1)
  • TheSEED  :
    • Sodium/di- and tricarboxylate cotransporter
  • PFAM:
    IT (CL0182) Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 367.8)
    and 1 more
    CitMHS; Citrate transporter (PF03600; HMM-score: 33.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015225
    • TAT(Tat/SPI): 0.000673
    • LIPO(Sec/SPII): 0.00453
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPSSLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPKSGSAILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLSIVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLIITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYFIMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPIDSASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSDQTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQSIGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSMTYWKWLGLL

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]