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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0750 [new locus tag: SACOL_RS03850 ]
  • pan locus tag?: SAUPAN002574000
  • symbol: SACOL0750
  • pan gene symbol?:
  • synonym:
  • product: anion transporter family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0750 [new locus tag: SACOL_RS03850 ]
  • symbol: SACOL0750
  • product: anion transporter family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 771081..772634
  • length: 1554
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCAGAAGAAAAACATGTAGTTGAACATGAACAACAAAAGAAAGAAAAGACAAAAAAG
    CAATACAAGCCATTTTGGATTGTCATGAGTTTTATAATACTTATAGTTGTACTATTACTC
    CCGGCACCTTCAAGTCTGCCGATAATGGCTAAGGCAGTACTAGCTATTTTAGCTTTTGCA
    GTTATTATGTGGGTAACGGAAGCTGTATCATATCCGGTGTCAGCAACTTTAATTATTGGC
    TTAATGATATTACTTTTAGGATTTAGCCCTGTTCAAAATTTAGGGGAGAAGCTAGGTAAT
    CCGAAAAGTGGCAGTGCTATTTTAGCTGGAAGTGACCTTCTAGGAACTAATCATGCATTA
    TCATTAGCGTTTAGTGGATTTGCAACTTCAGCTGTAGCTCTCGTTGCAGCTGCATTATTT
    TTGGCTGCTGCTATGCAAGAAACGAATTTGCATAAAAGACTAGCTCTTTTAGTGTTATCA
    ATTGTTGGTAATAAAACTAGAAATATAGTTATTGGAGCAATTATCGTTTCAATTGTACTT
    GCATTTTTCGTTCCTTCTGCAACAGCTAGAGCAGGGGCAGTTGTACCAATCTTGCTGGGT
    ATGATTGCGGCATTTAAAGTTTCCAAAGATAGCAAGTTAGCGTCTTTATTAATAATTACT
    TCAGTACAAGCTGTGTCAATTTGGAATATTGGTATCAAAACGGCGGCAGCACAAAATATC
    GTAGCGATTAATTTTATAAACCATCAATTAGGATTTGATGTTTCATGGGGCGAGTGGTTC
    TTATATGCAGCGCCTTGGTCCATAGTTATGTCCGTAGCTTTATATTTCATCATGATTAAA
    GTGATGCCTCCAGAAATTAATACAATAGAAGGTGGTAAAGATTTAATAAAAGAAGAATTG
    CATAAACTTGGCCCCGTTAGCCCACGTGAATGGCGTTTAATTGTTATATCGATGTTATTA
    TTACTGTTTTGGTCAACTGAAAAAGTATTACATCCGATTGACTCTGCATCCATTACTATT
    ATTGCTTTAGGTGTTATGTTAATGCCGAAAATTGGTGTCATGACATGGAAACATGTTGAA
    AATAAAATACCATGGGGAACAATTATCGTGTTTGGTGTAGGTATTTCACTAGGTAACGTT
    CTTTTGAAAACAGGTGCAGCTCAATGGTTAAGTGATCAAACTTTTGGTGTTTTAGGTTTA
    AAACATTTACCTATTATCGCGACAATTGCACTTATCACGCTTTTTAATATATTGATTCAT
    TTGGGCTTTGCGAGTGCAACAAGTTTATCATCAGCGTTAATACCTGTTTTTATTTCGCTA
    ACCTCTACGTTACACTTAGGAGACCAGTCTATAGGATTTGTTTTAATTCAACAATTTGTT
    ATTAGTTTTGGTTTCTTATTACCTGTTAGTGCACCTCAAAATATGTTGGCTTATGGCACT
    GGTACTTTTACGGTTAAAGATTTCTTGAAGGCAGGTATACCATTGACAATTGTAGGGTAT
    ATTCTAGTGATAGTTTTTAGCATGACTTATTGGAAATGGTTAGGTTTGCTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0750 [new locus tag: SACOL_RS03850 ]
  • symbol: SACOL0750
  • description: anion transporter family protein
  • length: 517
  • theoretical pI: 10.1501
  • theoretical MW: 55863.7
  • GRAVY: 0.889168

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257.2)
    MetabolismTransport and binding proteinsAnionstransporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257.2)
    TIGR03943 family protein (TIGR03943; HMM-score: 5.6)
  • TheSEED:  
    Sodium/di- and tricarboxylate cotransporter 
  • PFAM:
    IT (CL0182) Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 367.3)
    CitMHS; Citrate transporter (PF03600; HMM-score: 34.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.169
    • Ymax_pos: 53
    • Cmax: 0.169
    • Cmax_pos: 53
    • Smax: 0.314
    • Smax_pos: 36
    • Smean: 0.155
    • D: 0.164
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPSSLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPKSGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLSIVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLIITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYFIMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPIDSASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSDQTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQSIGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSMTYWKWLGLL

Experimental data[edit source | edit]

  • experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]