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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0698 [new locus tag: SA_RS03975 ]
  • pan locus tag?: SAUPAN002650000
  • symbol: pepT
  • pan gene symbol?: pepT
  • synonym:
  • product: peptidase T

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0698 [new locus tag: SA_RS03975 ]
  • symbol: pepT
  • product: peptidase T
  • replicon: chromosome
  • strand: -
  • coordinates: 796283..797509
  • length: 1227
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAGAACCAATTGATAGATAGATTAACAAGATATACGACAATTGATACGCAATCTGAT
    CCAAAATCTACAACAACACCTTCAACTGAAAAGCAATGGGATTTGTTACATTTATTAGAA
    AAAGAATTACAACAATTAGGATTGCCAACTGATTTAGATGAAAATGGCTACTTATTTGCT
    ACATTAGAAAGTAACATCGATGCCGACGTCCCAACAGTTGGATTTTTGGCGCATGTTGAT
    ACATCACCTGATTTCAATGCTTCAAATGTTAAACCGCAAATTATTGAAAACTATGATGGT
    AAGCCATACAAACTAGGCAATACTAAACGTGTCTTAGATCCTAAAGTATTTCCAGAACTT
    AACAGTTTAGTTGGTCATACATTAATGGTAACTGATGGTACATCGTTACTAGGTGCAGAC
    GATAAAGCGGGTATTGTGGAAATTATGGAAGCTATTTGTTATTTACAAGAACATCCAGAA
    ATTAAACATGGTACCATTCGCATTGGATTTACACCAGACGAAGAAATCGGTCGTGGTCCA
    CATAAATTTGATGTTGACCGCTTCAATGCTGATTTTGCTTATACTATGGATGGTAGTCAA
    TATGGAGAATTACAATATGAAAGCTTTAACGCTGCTGAAGCGGTTATTACATGCCACGGT
    GTAAATGTTCATCCTGGTTCAGCTAAAAATGCAATGGTAAACGCAATACGTTTAGGTGAA
    CAATTCGATAGTTTGCTACCTGATAGTGAAGTTCCGGAGCGAACAGAAGGATACGAAGGC
    TTTTATCACTTAATGAACTTTGAAGGAACTGTTGAAAAAGCAACTTTGCAATACATTATT
    CGTGATCATGATAAAAAACAATTCGAATTGCGTAAGAAACGTATTTTAGAAATACGTGAC
    GATATCAATGCCCATTTTGAAAATTATCCAGTTAAAGTTGATATATCGGATCAATATTTC
    AATATGGCAGAAAAAATATTACCATTGCCTCATATTATTGATATACCTAAACGTGTCTTT
    GCCAAATTAGATATTCCAGCAAACACTGAACCTATTCGTGGTGGTACAGATGGTTCACAA
    TTGTCATTTATGGGGTTACCAACTCCAAACATCTTTACAGGTTGCGGTAACTTCCACGGC
    CCATATGAATATGCATCCATTGATGTTATGGAAAAAGCAGTACAAGTAATCATTGGAATT
    GTAGAAGATATCGCTGAAAATCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1227

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0698 [new locus tag: SA_RS03975 ]
  • symbol: PepT
  • description: peptidase T
  • length: 408
  • theoretical pI: 4.56391
  • theoretical MW: 45819.3
  • GRAVY: -0.404167

Function[edit source | edit]

  • reaction:
    EC 3.4.11.4?  ExPASy
    Tripeptide aminopeptidaseRelease of the N-terminal residue from a tripeptide
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase T (TIGR01882; EC 3.4.11.-; HMM-score: 554.4)
    peptidase T-like protein (TIGR01883; HMM-score: 106.3)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 24.3)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesamidohydrolase (TIGR01891; HMM-score: 14.7)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 13.2)
    amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 11.2)
    hydrolase, peptidase M42 family (TIGR03106; HMM-score: 11.1)
    N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 10.5)
  • TheSEED:  
    Stress ResponseOxidative stressGlutathione: Biosynthesis and gamma-glutamyl cycle Tripeptide aminopeptidase (EC 3.4.11.4) 
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 46.9)
    no clan definedM20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 37.9)
    Peptidase_MH (CL0035) Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 25.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 20
    • Cmax: 0.108
    • Cmax_pos: 33
    • Smax: 0.184
    • Smax_pos: 13
    • Smean: 0.148
    • D: 0.135
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQQLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKPYKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPEIKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITCHGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATLQYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIIDIPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEKAVQVIIGIVEDIAENH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]