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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00757
  • pan locus tag?: SAUPAN002650000
  • symbol: SAOUHSC_00757
  • pan gene symbol?: pepT
  • synonym:
  • product: peptidase T

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00757
  • symbol: SAOUHSC_00757
  • product: peptidase T
  • replicon: chromosome
  • strand: -
  • coordinates: 740290..741516
  • length: 1227
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAGAACCAATTGATAGATAGATTAACAAGATATACGACAATTGATACGCAATCTGAT
    CCAAAATCTACAACAACACCTTCAACTGAAAAGCAATGGGATTTGTTACATTTATTAGAA
    AAAGAATTACAACAATTAGGATTGCCAACTGATTTAGATGAAAATGGCTACTTATTTGCT
    ACATTAGAAAGTAACATCGATGTCGACGTCCCAACAGTTGGATTTTTGGCGCATGTTGAT
    ACATCACCTGATTTCAATGCTTCAAATGTTAAACCGCAAATTATTGAAAACTATGATGGT
    AAGCCATACAAACTAGGCAATACTAAACGTGTCTTAGATCCTAAAGTATTTCCAGAACTT
    AACAGTTTAGTTGGTCATACATTAATGGTAACTGATGGTACATCGTTACTAGGTGCAGAC
    GATAAAGCGGGTATTGTGGAAATTATGGAAGCTATTTGTTATTTACAAGAACATCCAGAA
    ATTAAACATGGTACCATTCGCATTGGATTTACACCAGACGAAGAAATCGGTCGTGGTCCA
    CATAAATTTGATGTTGACCGCTTCAATGCTGATTTTGCTTATACTATGGATGGTAGTCAA
    TATGGAGAATTACAATATGAAAGCTTTAACGCTGCTGAAGCGGTTATTACATGCCACGGT
    GTAAATGTTCATCCTGGTTCAGCTAAAAATGCAATGGTAAACGCAATACGTTTAGGTGAA
    CAATTCGATAGTTTGCTACCTGATAGTGAAGTTCCGGAGCGAACAGAAGGATACGAAGGC
    TTTTATCACTTAATGAACTTTGAAGGAACTGTTGAAAAAGCAACTTTGCAATACATTATT
    CGTGATCATGATAAAAAACAATTCGAATTGCGTAAGAAACGTATTTTAGAAATACGTGAC
    GATATCAATGCCCATTTTGAAAATTATCCAGTTAAAGTTGATATATCGGATCAATATTTC
    AATATGGCAGAAAAAATATTACCATTGCCTCATATTATTGATATACCTAAACGTGTCTTT
    GCCAAATTAGATATTCCAGCAAACACTGAACCTATTCGCGGTGGTACAGATGGTTCACAA
    TTGTCATTTATGGGGTTACCAACTCCAAACATCTTTACAGGTTGCGGTAACTTCCACGGC
    CCATATGAATATGCATCCATTGATGTTATGGAAAAAGCAGTACAAGTAATCATTGGAATT
    GTAGAAGATATCGCTGAAAATCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1227

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00757
  • symbol: SAOUHSC_00757
  • description: peptidase T
  • length: 408
  • theoretical pI: 4.56391
  • theoretical MW: 45847.3
  • GRAVY: -0.398284

Function[edit source | edit]

  • reaction:
    EC 3.4.11.4?  ExPASy
    Tripeptide aminopeptidaseRelease of the N-terminal residue from a tripeptide
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase T (TIGR01882; EC 3.4.11.-; HMM-score: 554.1)
    peptidase T-like protein (TIGR01883; HMM-score: 107.2)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 24.3)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesamidohydrolase (TIGR01891; HMM-score: 14.6)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 13.2)
    amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 11)
  • TheSEED:  
    Stress ResponseOxidative stressGlutathione: Biosynthesis and gamma-glutamyl cycle Tripeptide aminopeptidase (EC 3.4.11.4) 
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 46.7)
    no clan definedM20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 37.9)
    Peptidase_MH (CL0035) Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 25.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 20
    • Cmax: 0.108
    • Cmax_pos: 33
    • Smax: 0.178
    • Smax_pos: 13
    • Smean: 0.144
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQQLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKPYKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPEIKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITCHGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATLQYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIIDIPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEKAVQVIIGIVEDIAENH

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]