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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0730 [new locus tag: SA_RS04155 ]
  • pan locus tag?: SAUPAN002709000
  • symbol: pgm
  • pan gene symbol?: pgm
  • synonym:
  • product: phosphoglyceromutase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0730 [new locus tag: SA_RS04155 ]
  • symbol: pgm
  • product: phosphoglyceromutase
  • replicon: chromosome
  • strand: +
  • coordinates: 835910..837427
  • length: 1518
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCTAAGAAACCAACTGCGTTAATTATTTTAGATGGTTTTGCGAACCGCGAAAGCGAA
    CATGGTAATGCGGTAAAATTAGCAAACAAGCCTAATTTTGATCGTTATTACAACAAATAT
    CCAACGACTCAAATCGAAGCGAGTGGCTTAGATGTTGGACTACCTGAAGGACAAATGGGT
    AACTCAGAAGTTGGTCATATGAATATCGGTGCAGGACGTATCGTTTATCAAAGTTTAACT
    CGAATCAATAAATCAATTGAAGACGGTGATTTCTTTGAAAATGATGTTTTAAATAATGCA
    ATTGCACACGTGAATTCACATGATTCAGCGTTACACATCTTTGGTTTATTGTCTGACGGT
    GGTGTGCACAGTCATTACAAACATTTATTTGCTTTGTTAGAACTTGCTAAAAAACAAGGT
    GTTGAAAAAGTTTACGTACACGCATTTTTAGATGGTCGTGACGTAGATCAAAAATCCGCT
    TTGAAATACATCGAAGAGACTGAAGCTAAATTCAATGAATTAGGCATTGGTCAATTTGCA
    TCTGTGTCTGGTCGTTATTATGCAATGGACCGTGACAAACGTTGGGAACGTGAAGAAAAA
    GCTTACAATGCTATTCGTAATTTTGATGCCCCAACTTATGCAACTGCCAAAGAAGGTGTA
    GAAGCAAGCTATAATGAGGGCTTAACTGACGAATTCGTAGTACCATTCATCGTTGAGAAT
    CAAAATGACGGTGTTAATGATGGAGATACAGTGATCTTCTATAATTTCCGACCTGATAGA
    GCAGCACAATTATCGGAAATTTTTGCGAACAGAGCATTCGAAGGCTTTAAAGTTGAACAA
    GTTAAAGACTTATTCTATGCAACATTCACTAAGTATAACGACAATATCGATGCGGCTATC
    GTTTTCGAAAAAGTTGATTTAAATAATACAATTGGTGAAATTGCACAAAATAACAATTTA
    ACACAATTACGTATTGCAGAAACTGAAAAATATCCTCACGTTACTTACTTTATGAGTGGT
    GGACGTAACGAGGAATTTAAAGGTGAACGCCGTCGTTTAATTGATTCACCTAAAGTTGCA
    ACGTATGACTTGAAACCAGAAATGAGTGCTTATGAAGTTAAAGATGCATTATTAGAAGAG
    TTAAATAAAGGTGACTTGGACTTAATTATTTTAAACTTTGCTAACCCTGATATGGTTGGA
    CATAGTGGTATGCTTGAGCCGACAATCAAAGCAATCGAAGCGGTTGATGAATGTTTAGGT
    GAAGTCGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGT
    AACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCA
    GTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTCGAGAAACTGGTCGCTTAGGTGAC
    TTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGACATGACAGGTGAA
    TCTTTAATTAAACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1518

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0730 [new locus tag: SA_RS04155 ]
  • symbol: Pgm
  • description: phosphoglyceromutase
  • length: 505
  • theoretical pI: 4.49265
  • theoretical MW: 56453.7
  • GRAVY: -0.432277

Function[edit source | edit]

  • reaction:
    EC 5.4.2.12?  ExPASy
    Phosphoglycerate mutase (2,3-diphosphoglycerate-independent)2-phospho-D-glycerate = 3-phospho-D-glycerate
    EC 5.4.2.1?  ExPASy
    Transferred entry: 5.4.2.11 and 5.4.2.12
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphoglycerate mutase (2,3-diphosphoglycerate-independent) (TIGR01307; EC 5.4.2.12; HMM-score: 694)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphoglycerate mutase (2,3-diphosphoglycerate-independent), archaeal form (TIGR00306; EC 5.4.2.12; HMM-score: 51.7)
    proposed homoserine kinase (TIGR02535; HMM-score: 28.2)
  • TheSEED:  
    Glycolysis and Gluconeogenesis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.12) 
  • PFAM:
    no clan definediPGM_N; BPG-independent PGAM N-terminus (iPGM_N) (PF06415; HMM-score: 278.1)
    Alk_phosphatase (CL0088) Metalloenzyme; Metalloenzyme superfamily (PF01676; HMM-score: 257.5)
    Phosphodiest; Type I phosphodiesterase / nucleotide pyrophosphatase (PF01663; HMM-score: 22.7)
    PglZ; PglZ domain (PF08665; HMM-score: 17.2)
    Sulfatase; Sulfatase (PF00884; HMM-score: 16.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.184
    • Ymax_pos: 28
    • Cmax: 0.132
    • Cmax_pos: 28
    • Smax: 0.445
    • Smax_pos: 17
    • Smean: 0.204
    • D: 0.192
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQIEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIAHVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSALKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKEGVEASYNEGLTDEFVVPFIVENQNDGVNDGDTVIFYNFRPDRAAQLSEIFANRAFEGFKVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHVTYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILNFANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDDQPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: GapR
    GapR (TF) important in Glycolysis  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]