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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0731 [new locus tag: SA_RS04160 ]
  • pan locus tag?: SAUPAN002710000
  • symbol: eno
  • pan gene symbol?: eno
  • synonym:
  • product: phosphopyruvate hydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0731 [new locus tag: SA_RS04160 ]
  • symbol: eno
  • product: phosphopyruvate hydratase
  • replicon: chromosome
  • strand: +
  • coordinates: 837557..838861
  • length: 1305
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCAATTATTACAGATGTTTACGCTCGCGAAGTCTTAGACTCTCGTGGTAACCCAACT
    GTTGAAGTAGAAGTATTAACTGAAAGTGGCGCATTTGGTCGTGCATTAGTACCATCAGGT
    GCTTCAACTGGTGAACACGAAGCTGTTGAATTACGTGATGGAGACAAATCACGTTATTTA
    GGTAAAGGTGTTACTAAAGCAGTTGAAAACGTTAATGAAATCATCGCACCAGAAATTATT
    GAAGGTGAATTTTCAGTATTAGATCAAGTATCTATTGATAAAATGATGATCGCATTAGAC
    GGTACTCCAAACAAAGGTAAATTAGGTGCAAATGCTATTTTAGGTGTATCTATCGCAGTA
    GCACGTGCAGCAGCTGACTTATTAGGTCAACCACTTTACAAATATTTAGGTGGATTTAAT
    GGTAAGCAGTTACCAGTACCAATGATGAACATCGTTAATGGTGGTTCTCACTCAGATGCT
    CCAATTGCATTCCAAGAATTCATGATTTTACCTGTAGGTGCTACAACGTTCAAAGAATCA
    TTACGTTGGGGTACTGAAATTTTCCACAACTTAAAATCAATTTTAAGCAAACGTGGTTTA
    GAAACTGCAGTAGGTGACGAAGGTGGTTTCGCTCCTAAATTTGAAGGTACTGAAGATGCT
    GTTGAAACAATTATCCAAGCAATCGAAGCAGCTGGTTACAAACCAGGTGAAGAAGTATTC
    TTAGGATTTGACTGTGCATCATCAGAATTCTATGAAAATGGTGTATATGACTACAGTAAG
    TTCGAAGGCGAACACGGTGCAAAACGTACAGCTGCAGAACAAGTTGACTACTTAGAACAA
    TTAGTAGACAAATATCCTATCATTACAATTGAAGACGGTATGGACGAAAACGACTGGGAT
    GGTTGGAAACAACTTACAGAACGTATCGGTGACCGTGTACAATTAGTAGGTGACGATTTA
    TTCGTAACAAACACTGAAATTTTAGCAAAAGGTATTGAAAACGGAATTGGTAACTCAATC
    TTAATTAAAGTTAACCAAATCGGTACATTAACTGAAACATTTGATGCAATCGAAATGGCT
    CAAAAAGCTGGTTACACAGCAGTAGTTTCTCACCGTTCAGGTGAAACAGAAGATACAACA
    ATTGCTGATATTGCTGTTGCTACAAACGCTGGTCAAATTAAAACTGGTTCATTATCACGT
    ACTGACCGTATTGCTAAATACAATCAATTATTACGTATCGAAGATGAATTATTTGAAACT
    GCTAAATATGACGGTATCAAATCATTCTATAACTTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1305

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0731 [new locus tag: SA_RS04160 ]
  • symbol: Eno
  • description: phosphopyruvate hydratase
  • length: 434
  • theoretical pI: 4.27961
  • theoretical MW: 47116.6
  • GRAVY: -0.200461

Function[edit source | edit]

  • reaction:
    EC 4.2.1.11?  ExPASy
    Phosphopyruvate hydratase2-phospho-D-glycerate = phosphoenolpyruvate + H2O
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphopyruvate hydratase (TIGR01060; EC 4.2.1.11; HMM-score: 690.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneo-succinylbenzoate synthase (TIGR01928; EC 4.2.1.113; HMM-score: 17.8)
    MetabolismEnergy metabolismFermentationmethylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)
    MetabolismEnergy metabolismAmino acids and aminesmethylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismDehydrogenase complexes Enolase (EC 4.2.1.11) 
    Central carbohydrate metabolismGlycolysis and Gluconeogenesis Enolase (EC 4.2.1.11) 
    One-carbon MetabolismSerine-glyoxylate cycle Enolase (EC 4.2.1.11) 
  • PFAM:
    Enolase_TIM (CL0256) Enolase_C; Enolase, C-terminal TIM barrel domain (PF00113; HMM-score: 434.7)
    Enolase_N (CL0227) Enolase_N; Enolase, N-terminal domain (PF03952; HMM-score: 204.8)
    Enolase_TIM (CL0256) MAAL_C; Methylaspartate ammonia-lyase C-terminus (PF07476; HMM-score: 26.1)
    MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 25.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:
    SA0865(ppnK)inorganic polyphosphate/ATP-NAD kinase  [2] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [2] (data from MRSA252)
    SA2040(rplP)50S ribosomal protein L16  [2] (data from MRSA252)
    SA2022(rplQ)50S ribosomal protein L17  [2] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [2] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA2016(rpsI)30S ribosomal protein S9  [2] (data from MRSA252)
    SA0342hypothetical protein  [2] (data from MRSA252)
    SA0627hypothetical protein  [2] (data from MRSA252)
    SA2099hypothetical protein  [2] (data from MRSA252)
    SA2123hypothetical protein  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.155
    • Ymax_pos: 42
    • Cmax: 0.206
    • Cmax_pos: 42
    • Smax: 0.204
    • Smax_pos: 41
    • Smean: 0.097
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: GapR
    GapR (TF) important in GlycolysisRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol. Microbiol.: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] (P p)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)