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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0828 [new locus tag: SA_RS04705 ]
  • pan locus tag?: SAUPAN003077000
  • symbol: SA0828
  • pan gene symbol?: addA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0828 [new locus tag: SA_RS04705 ]
  • symbol: SA0828
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 935854..939507
  • length: 3654
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATT
    TACGCAACTGGACAAGATGTACTTGTTGCAGCCGCGGCAGGTTCAGGTAAAACAGCTGTA
    CTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCATTGATGTCGATCGACTTTTA
    GTCGTAACGTTTACAAACTTAAGCGCACGTGAAATGAAGCATCGTGTAGACCAACGTATT
    CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGCATCAAAATT
    CATCAAGCACAAATATCTACACTTCATAGTTTTTGCTTGAAATTAATTCAACAGCATTAT
    GATGTATTAAATATTGACCCGAACTTTAGAACAAGCAGTGAAGCTGAAAATATTTTATTA
    TTAGAACAAACGATAGATGAGGTCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
    ATTGAATTAACAGAACAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATT
    AAACAATTGTATTTCTTTAGCGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTG
    GTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTATTCAACTACTAACAGACTTA
    TCTAAAGTATTTATCACAGCTGCCTATGATGCTTTAAATAAGGCGTATGATTTGTTTAGT
    ATGATGGATGGCGTCGATAAACATTTAGCTGTTATAGAAGATGAACGACGTTTAATGGGG
    CGTGTTTTAGAAGGTGGTTTTATTGATATACCTTATTTAACTGATCACGAATTTGGCGCG
    CGTTTGCCTAATGTAACAGCGAAAATTAAAGAAGCAAATGAAATGATGGTCGATGCCTTA
    GAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTGAAAAATGAT
    TACTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAATTGGCGCCACGAGTA
    AAGTACCTTGCGCGTATTGTGAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGC
    AAAAATATTCTGGATTTTTCTGATTATGAACATTTTGCATTACAAATTTTAACTAATGAG
    GATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACACTTTCAAGAAATATTGGTC
    GATGAGTATCAAGATACGAACCGGGTTCAAGAGAAAATACTATCTTGCATCAAAACGGGT
    GATGAACATAATGGTAATTTATTTATGGTTGGAGATGTTAAGCAATCCATTTATAAATTT
    AGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAACGCTTTACTATAGATGGAGAT
    GGCACTGGACGTCGAATTGATTTGTCGCAAAACTTCCGTTCTCGAAAAGAAGTACTGTCA
    ACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGAT
    GAAGCGGCACAGTTGTATTATGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTA
    AAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAACTGGTAGTGAACAAGAAGCG
    CATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAA
    ACAGGAAGCTATAGAAGTGCGACATACAAAGATATCGTTATTCTAGAACGCAGCTTTGGA
    CAAGCTCGCAATTTACAACAAGCCTTTAAAAATGAAGATATTCCATTCCATGTGAATAGT
    CGTGAAGGTTACTTTGAACAAACAGAAGTCCGCTTAGTATTATCATTTTTAAGAGCGATA
    GATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGCTCCGTTATATATCAGTTC
    AAAGAAGACGAATTAGCTCAAATTAGAATATTGAGTCCAAATGATGACTACTTCTATCAA
    TCGATTGTAAATTACATTAATGACGAAGCAGCAGATGCTATTTTAGTTGATAAATTAAAA
    ATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTAAAGATCATCCGGTGTATCAG
    TTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
    GGACGTGGACGACGTGCAAACCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAAT
    TCAAGTTTTAGAGGTTTATATCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGC
    AAAGATTTTGGTGAGGAAAATGTAGTTGGTCCAAACGATAATGTTGTTAGAATGATGACA
    ATTCATAGTAGTAAAGGTCTAGAGTTTCCATTTGTCATTTATTCTGGATTGTCAAAAGAT
    TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATG
    GATTATTTTGATGTGGATAAAGAAATGGCATTTCCATCTTTAGCTTCGGTTGCATATAAA
    GCTGTTGCCGAAAAAGAACTTGTGTCAGAAGAAATGCGATTAGTCTATGTAGCATTAACA
    AGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCGTTACTAGAA
    CTAGAGCAATTGTCTATTTCTGGTGAGCACATTGCTGTCAATGAACGATTAACTTCACCA
    AATCCGTTCCATCTTATTTATAGTATTTTATCTAAACATCAATCTGCGTCAATTCCAGAT
    GATTTAAAATTTGAAAAAGATATAGCACAAGTTGAAGATAGTAGTCGTCCGAATGTAAAT
    ATTTCAATTATATACTTTGAAGATGTGTCTACAGAAACCATTTTAGATAATAATGAATAT
    CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAGGATGTTAAAGCACAAATT
    AAACACCAACTTGATTATCAATATCCATATGTAAATGATACTAAAAAGCCATCAAAACAA
    TCTGTTTCTGAATTGAAAAGGCAATATGAAACAGAAGAAAGTGGCACAAGTTACGAACGA
    GTAAGACAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
    GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTGATGCAACATTTACCA
    TTCAAAAAAGAACGCATATCTGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGAT
    AAACATATTATCGAAGCAGATGCGAAAAAAGATATCCGTATGGATGAAATAATGACATTT
    ATCAATAGTGAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
    TTTGTAGTTAACCAAGCATTAGTTGACCAATTGCCACAAGGAGACGAAGACGTCTCAATT
    ATTCAAGGTATGATTGACTTAATCTTTGTTAAAGATGGTGTGCATTATTTTGTAGACTAT
    AAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATGAAGAAATTGGTACACAATTA
    AAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATACTTAAT
    AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3654

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0828 [new locus tag: SA_RS04705 ]
  • symbol: SA0828
  • description: hypothetical protein
  • length: 1217
  • theoretical pI: 4.92652
  • theoretical MW: 141258
  • GRAVY: -0.471159

Function[edit source | edit]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicaseATP + H2O = ADP + phosphate
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairhelicase-exonuclease AddAB, AddA subunit (TIGR02785; EC 3.1.-.-,3.6.4.12; HMM-score: 1457.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairdouble-strand break repair helicase AddA (TIGR02784; HMM-score: 214.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexodeoxyribonuclease V, beta subunit (TIGR00609; EC 3.1.11.5; HMM-score: 214.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 210.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA helicase II (TIGR01075; EC 3.6.4.12; HMM-score: 195.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 191)
  • TheSEED:  
    DNA MetabolismDNA repairATP-dependent Nuclease ATP-dependent nuclease, subunit A 
  • PFAM:
    P-loop_NTPase (CL0023) UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 271)
    UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 112.3)
    AAA_19; AAA domain (PF13245; HMM-score: 75.5)
    UvrD_C_2; UvrD-like helicase C-terminal domain (PF13538; HMM-score: 38)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 28.7)
    PDDEXK (CL0236) PDDEXK_1; PD-(D/E)XK nuclease superfamily (PF12705; HMM-score: 25.8)
    P-loop_NTPase (CL0023) Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 22.2)
    AAA_12; AAA domain (PF13087; HMM-score: 19.5)
    no clan definedOccludin_ELL; Occludin homology domain (PF07303; HMM-score: 16.4)
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.2)
    no clan definedDUF2564; Protein of unknown function (DUF2564) (PF10819; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.104
    • Ymax_pos: 32
    • Cmax: 0.132
    • Cmax_pos: 32
    • Smax: 0.162
    • Smax_pos: 31
    • Smean: 0.055
    • D: 0.085
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAFIELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTDHEFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKNDYFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVKGYLYFFKFGTLQL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]