From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0875 [new locus tag: SA_RS04945 ]
  • pan locus tag?: SAUPAN003195000
  • symbol: SA0875
  • pan gene symbol?: ugtP
  • synonym:
  • product: diacylglycerol glucosyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0875 [new locus tag: SA_RS04945 ]
  • symbol: SA0875
  • product: diacylglycerol glucosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 990883..992058
  • length: 1176
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGTTACTCAAAATAAAAAGATATTGATTATTACTGGCTCATTCGGTAACGGTCATATG
    CAAGTTACACAGAGTATCGTTAATCAACTTAATGATATGAATCTAGACCATTTAAGCGTC
    ATTGAGCACGATTTATTTATGGAAGCTCATCCAATTTTGACTTCTATTTGTAAAAAATGG
    TATATCAATAGCTTTAAATATTTTAGAAATATGTACAAAGGGTTTTATTACAGCCGCCCA
    GATAAACTAGACAAATGTTTTTACAAATACTATGGACTTAATAAGTTAATTAATTTATTG
    ATAAAAGAAAAGCCAGATTTAATATTATTAACGTTTCCTACACCAGTTATGTCGGTACTA
    ACTGAGCAATTTAACATTAATATTCCAGTTGCTACAGTGATGACAGACTATCGCTTACAT
    AAAAACTGGATTACGCCGTATTCAACAAGATATTATGTGGCAACAAAAGAAACGAAACAA
    GACTTCATAGACGTAGGTATTGATCCTTCAACAGTTAAAGTGACAGGTATTCCTATTGAT
    AACAAATTTGAAACGCCTATTAATCAAAAGCAGTGGTTAATAGACAACAACTTAGATCCA
    GATAAGCAAACTATTTTAATGTCAGCTGGTGCATTTGGTGTATCTAAAGGTTTTGACACG
    ATGATTACTGATATATTAGCGAAAAGTGCAAATGCACAAGTAGTTATGATTTGTGGTAAG
    AGCAAAGAGCTAAAGCGTTCTTTAATAGCTAAGTTTAAATCTAACGAGAATGTATTGATT
    CTAGGTTATACCAAACACATGAATGAATGGATGGCATCAAGTCAACTTATGATTACGAAA
    CCTGGTGGTATCACAATAACTGAAGGTTTCGCCCGTTGTATTCCAATGATTTTCCTAAAT
    CCTGCACCTGGTCAAGAGCTTGAAAATGCCCTTTACTTTGAAGAAAAAGGTTTTGGTAAA
    ATCGCTGATACTCCAGAAGAAGCAATAAAAATTGTAGCAAGTTTAACGAACGGTAATGAG
    CAATTAACTAACATGATTAGTACAATGGAACAAGATAAAATTAAGTATGCTACACAGACA
    ATATGTCGAGACTTACTTGATTTAATCGGTCATTCATCACAACCACAAGAGATTTATGGA
    AAGGTTCCTTTATATGCAAGATTCTTCGTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1176

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0875 [new locus tag: SA_RS04945 ]
  • symbol: SA0875
  • description: diacylglycerol glucosyltransferase
  • length: 391
  • theoretical pI: 8.60534
  • theoretical MW: 44559.5
  • GRAVY: -0.167263

Function[edit source | edit]

  • reaction:
    EC 2.4.1.315?  ExPASy
    Diglucosyl diacylglycerol synthase (1,6-linking)UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDPUDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP
    UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol?
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanundecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 40.7)
    UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase (TIGR03590; EC 3.6.1.57; HMM-score: 22.8)
    Hypothetical proteinsConservedconserved hypothetical protein (TIGR00661; HMM-score: 20.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 12.4)
  • TheSEED:  
    Teichoic and lipoteichoic acids biosynthesis Processive diacylglycerol beta-glucosyltransferase (EC 2.4.1.315) 
  • PFAM:
    GT-B (CL0113) MGDG_synth; Monogalactosyldiacylglycerol (MGDG) synthase (PF06925; HMM-score: 163.4)
    Glyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 40.3)
    Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 34.4)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 22.1)
    Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 19.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.186
    • Ymax_pos: 25
    • Cmax: 0.123
    • Cmax_pos: 28
    • Smax: 0.365
    • Smax_pos: 20
    • Smean: 0.266
    • D: 0.218
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGKSKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]