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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0879 [new locus tag: SA_RS04970 ]
  • pan locus tag?: SAUPAN003203000
  • symbol: htrA
  • pan gene symbol?: htrA2
  • synonym:
  • product: serine protease HtrA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0879 [new locus tag: SA_RS04970 ]
  • symbol: htrA
  • product: serine protease HtrA
  • replicon: chromosome
  • strand: +
  • coordinates: 997117..999426
  • length: 2310
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    GTGGATATTGGTAAAAAACATGTAATTCCTAAAAGTCAGTACCGACGTAAGCGTCGTGAA
    TTCTTCCACAACGAAGACAGAGAAGAAAATTTAAATCAACATCAAGATAAACAAAATATA
    GATAATACAACATCAAAAAAAGCAGATAAGCAAATACATAAAGATTCAATTGATAAGCAC
    GAACGTTTTAAAAATAGTTTATCATCGCATTTAGAACAGAGAAACCGTGATGTTAATGAG
    AATAAAGCTGAAGAAAGTAAAAGTAATCAGGATAGTAAGTCAGCATATAACAGAGATCAT
    TATTTAACAGACGATGTATCTAAAAAACAAAATTCATTAGATTCAGTGGACCAAGATACA
    GAGAAATCAAAATATTATGAGCAAAATTCTGAAGCGACTTTATCAACTAAATCAACCGAT
    AAAGTAGAATCAACTGAAATGAGAAAGCTAAGTTCAGATAAAAACAAAGTTGGTCATGAA
    GAGCAACATGTACTTTCTAAACCTTCAGAACATGATAAAGAGACTAGAATTGATTCTGAG
    TCTTCAAGAACTGATTCAGACAGCTCGATGCAGACAGAGAAAATAAAAAAAGACAGTTCA
    GATGGAAATAAAAGTAGTAATCTGAAATCTGAAGTAATATCAGACAAATCAAATACAGTA
    CCAAAATTGTCGGAATCTGATGATGAAGTAAATAATCAGAAGCCATTAACTTTACCGGAA
    GAACAGAAATTGAAAAGACAGCAAAGTCAAAATGAGCAAACAAAAACCTATACATATGGT
    GATAGCGAACAAAATGACAAGTCTAATCATGAAAATGATTTAAGTCATCATACACCATCG
    ATAAGTGATGATAAAGATAACGTTATGAGAGAAAATCATATTGTTGACGATAATCCTGAT
    AATGATATCAATACACTATCATTATCAAAAATAGATGACGATCGAAAACTTGATGAAAAA
    ATTCATGTTGAAGATAAACATAAACAAAATGCAGACTCGTCTGAAACGGTGGGATATCAA
    AGTCAGTCAACTGCATCTCATCGTAGCACTGAAAAAAGAAATATTTCTATTAATGACCAT
    GATAAATTAAACGGTCAAAAAACAAATACAAAGACATCGGCAAATAATAATCAAAAAAAG
    GCTACATCAAAATTGAACAAAGGGCGCGCTACGAATAATAATTATAGTGACATTTTGAAA
    AAGTTTTGGATGATGTATTGGCCTAAATTAGTTATTCTAATGGGTATTATTATTCTAATT
    GTTATTTTGAATGCCATTTTTAATAATGTGAACAAAAATGATCGCATGAATGATAATAAT
    GATGCAGATGCTCAAAAATATACGACAACGATGAAAAATGCCAATAACACAGTTAAATCG
    GTCGTTACAGTTGAAAATGAAACATCAAAAGATTCATCATTACCTAAAGATAAAGCATCT
    CAAGACGAAGTGGGATCAGGTGTTGTATATAAAAAATCTGGAGATACGTTATATATTGTT
    ACGAATGCACACGTTGTCGGTGATAAAGAAAATCAAAAAATAACTTTCTCGAATAATAAA
    AGTGTTGTTGGGAAAGTGCTTGGTAAAGATAAATGGTCAGATTTAGCTGTTGTTAAAGCA
    ACTTCTTCAGACAGTTCAGTGAAAGAGATAGCTATTGGAGATTCAAATAATTTAGTGTTA
    GGAGAGCCAATATTAGTCGTAGGTAATCCACTTGGTGTAGACTTTAAAGGCACTGTGACA
    GAAGGTATTATTTCAGGTCTGAACAGAAATGTTCCTATTGATTTCGATAAAGATAATAAA
    TATGATATGTTGATGAAAGCTTTCCAAATTGATGCATCAGTAAATCCAGGTAACTCGGGT
    GGTGCTGTCGTCAATAGAGAAGGAAAATTAATAGGTGTAGTTGCAGCTAAAATTAGTATG
    CCAAACGTTGAAAATATGTCATTTGCAATACCTGTTAATGAAGTACAAAAGATTGTAAAA
    GATTTAGAAACAAAAGGTAAAATTGACTATCCCGATGTAGGTGTTAAAATGAAGAATATT
    GCCAGTCTAAATAGTTTTGAAAGACAAGCAGTTAAATTGCCAGGAAAAGTTAAGAACGGT
    GTTGTTGTAGATCAAGTTGACAACAATGGTTTAGCAGATCAATCTGGTCTGAAAAAAGGT
    GATGTAATTACTGAATTAGATGGCAAACTTTTAGAAGATGATTTACGCTTTAGGCAGATT
    ATATTTAGTCATAAAGATGACTTGAAATCAATTACAGCGAAGATTTATAGAGATGGTAAA
    GAGAAAGAAATTAATATTAAACTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2310

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0879 [new locus tag: SA_RS04970 ]
  • symbol: HtrA
  • description: serine protease HtrA
  • length: 769
  • theoretical pI: 6.98599
  • theoretical MW: 86447.2
  • GRAVY: -1.03212

Function[edit source | edit]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 219.6)
    Genetic information processingProtein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 219.6)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 139.5)
    Signal transductionRegulatory functionsProtein interactionsperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 139.5)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesRIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 17.9)
  • TheSEED:  
    Serine protease, DegP/HtrA, do-like (EC 3.4.21.-) 
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 107)
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 43.8)
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 42.8)
    PDZ-like (CL0466) PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 22.5)
    no clan definedDUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 15)
    Peptidase_PA (CL0124) Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 13.9)
    no clan definedDUF3671; Protein of unknown function (PF12420; HMM-score: 6.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 17
    • Cmax: 0.101
    • Cmax_pos: 40
    • Smax: 0.309
    • Smax_pos: 5
    • Smean: 0.201
    • D: 0.166
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQNIDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYNRDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKNKVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVISDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHENDLSHHTPSISDDKDNVMRENHIVDDNPDNDINTLSLSKIDDDRKLDEKIHVEDKHKQNADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNKGRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQKYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNAHVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLGEPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNSGGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKMKNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDLRFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]