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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1059 [new locus tag: SA_RS06010 ]
  • pan locus tag?: SAUPAN003499000
  • symbol: SA1059
  • pan gene symbol?: fmt
  • synonym:
  • product: methionyl-tRNA formyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1059 [new locus tag: SA_RS06010 ]
  • symbol: SA1059
  • product: methionyl-tRNA formyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1198301..1199236
  • length: 936
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTAAAATAATATTTATGGGTACACCAGACTTTTCAACAACTGTTTTAGAAATGCTT
    ATTGCAGAACATGATGTCATTGCAGTCGTAACGCAACCAGATCGACCTGTTGGACGTAAA
    CGTGTTATGACACCACCACCAGTTAAAAAAGTTGCAATGAAATATGATTTACCTGTATAT
    CAGCCTGAAAAATTAAGTGGATCAGAAGAATTAGAACAATTGCTTCAATTAGATGTAGAT
    TTAATTGTAACTGCTGCTTTTGGACAATTATTACCTGAATCATTGTTGGCATTACCAAAA
    CTTGGCGCAATTAATGTACATGCATCATTGTTACCAAAGTATAGAGGTGGTGCACCAATT
    CATCAGGCAATTATCGATGGTGAACAAGAAACCGGCATAACAATTATGTATATGGTTAAA
    AAATTAGATGCGGGTAATATTATTTCGCAACAAGCAATTAAAATAGAAGAAAATGATAAT
    GTCGGTACGATGCATGATAAATTAAGTGTATTAGGGGCAGATTTATTAAAAGAAACTTTA
    CCATCTATTATAGAGGGCACAAATGAAAGTGTACCTCAAGATGATACGCAAGCAACATTT
    GCTTCCAATATTCGACGCGAAGATGAGCGAATTAGCTGGAATAAACCAGGAAGACAAGTG
    TTTAATCAAATTCGTGGATTATCACCATGGCCAGTTGCTTATACAACTATGGATGACACT
    AACTTGAAAATATACGATGCTGAACTCGTTGAGACTAATAAGATAAACGAGCCTGGAACC
    ATTATAGAAACGACTAAAAAAGCCATTATTGTTGCTACAAATGATAATGAAGCTGTTGCA
    ATTAAAGATATGCAATTAGCTGGGAAAAAGAGAATGTTAGCTGCCAATTATTTAAGTGGT
    GCGCAAAACACACTAGTAGGGAAGAAACTTATATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1059 [new locus tag: SA_RS06010 ]
  • symbol: SA1059
  • description: methionyl-tRNA formyltransferase
  • length: 311
  • theoretical pI: 5.08754
  • theoretical MW: 34225.3
  • GRAVY: -0.161415

Function[edit source | edit]

  • reaction:
    EC 2.1.2.9?  ExPASy
    Methionyl-tRNA formyltransferase10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationmethionyl-tRNA formyltransferase (TIGR00460; EC 2.1.2.9; HMM-score: 356.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylglycinamide formyltransferase (TIGR00639; EC 2.1.2.2; HMM-score: 68.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisformyltetrahydrofolate deformylase (TIGR00655; EC 3.5.1.10; HMM-score: 57.3)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis Methionyl-tRNA formyltransferase (EC 2.1.2.9) 
    Protein MetabolismProtein biosynthesisTranslation initiation factors bacterial Methionyl-tRNA formyltransferase (EC 2.1.2.9) 
    Conserved gene cluster associated with Met-tRNA formyltransferase Methionyl-tRNA formyltransferase (EC 2.1.2.9) 
  • PFAM:
    no clan definedFormyl_trans_N; Formyl transferase (PF00551; HMM-score: 137.8)
    Formyl_trans_C; Formyl transferase, C-terminal domain (PF02911; HMM-score: 96.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.157
    • Ymax_pos: 23
    • Cmax: 0.131
    • Cmax_pos: 23
    • Smax: 0.281
    • Smax_pos: 6
    • Smean: 0.201
    • D: 0.174
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMTPPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLGAINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDNVGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGRQVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDNEAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)