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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1166 [new locus tag: SA_RS06620 ]
  • pan locus tag?: SAUPAN003702000
  • symbol: thrB
  • pan gene symbol?: thrB
  • synonym:
  • product: homoserine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1166 [new locus tag: SA_RS06620 ]
  • symbol: thrB
  • product: homoserine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1327841..1328755
  • length: 915
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCGAATGTTTTGGAGTTAACAATTCCTGCATCAACAGCCAACCTTGGAGTTGGCTTT
    GATTCTATAGGTATGGCTTTAGATAAATTTTTGCATCTGTCTGTAAAGGAAACATCAGGG
    ACAAAATGGGAATATATTTTCCATGATGATGCATCTAAGCAATTGCCTACTGACGAAACA
    AACTTTATTTATCATGTAGCACAACAAGTTGCTTCTAAATATAGTGTTGACTTGCCTATT
    TTATGTATCGAAATGAGAAGTGATATTCCATTGGCAAGAGGGTTAGGTTCGTCAGCTTCT
    GCTTTAGTAGGAGCTATATATATCGCAAATTATTTTGGTGATATCCAACTGTCTAAACAT
    GAGGTATTACAATTAGCGACTGAAATCGAAGGACATCCTGATAATGTTGCGCCGACCATT
    TATGGTGGTTTAATCGCTGGATTTTATAATGATGTCTCGAAAGAAACGTCAGTTGCACAT
    ATCGACATACCAGACGTGGATGTGATTGTAACGATACCAACTTATGAACTAAAAACAGAA
    GCATCAAGACGTGCTTTACCACAAAAATTAACACATAGTGAAGCGGTAAAAAGTAGTGCA
    ATTAGTAATACAATGATTTGTGCATTAGCACAGCACAATTATGAATTAGCAGGTAAACTC
    ATGCAACAAGATGGCTTTCATGAACCGTATCGTCAGCATTTAATTGCTGAATTTGATGAA
    GTGAAAACAATTGCTAGTCAACATAATGCCTATGCAACTGTAATTAGTGGTGCTGGACCA
    ACTATTTTAATATTTAGTCGTAAAGAAAATAGTGGGGAATTGGTTCGCTCTTTAAATAGT
    CAGGTAGTATCATGCCATTCTGAATTGGTCGATATTAATATCAGTGGTGTTAAAGAACGA
    ATTGTATACCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1166 [new locus tag: SA_RS06620 ]
  • symbol: ThrB
  • description: homoserine kinase
  • length: 304
  • theoretical pI: 5.15013
  • theoretical MW: 33249.4
  • GRAVY: -0.0243421

Function[edit source | edit]

  • reaction:
    EC 2.7.1.39?  ExPASy
    Homoserine kinaseATP + L-homoserine = ADP + O-phospho-L-homoserine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAspartate familyhomoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 260.8)
    MetabolismCentral intermediary metabolismOthermevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 46.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 29.3)
    MetabolismAmino acid biosynthesisAromatic amino acid familyshikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 28.3)
    MetabolismCentral intermediary metabolismOtherphosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 22.9)
    MetabolismCentral intermediary metabolismOtherdiphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 16.1)
    MetabolismEnergy metabolismSugarsgalactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 11.8)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis Homoserine kinase (EC 2.7.1.39) 
    Lysine, threonine, methionine, and cysteineThreonine and Homoserine Biosynthesis Homoserine kinase (EC 2.7.1.39) 
    CBSS-269482.1.peg.1294 Homoserine kinase (EC 2.7.1.39) 
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 52.9)
    no clan definedGHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 34.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 4.6
    • Cellwall Score: 2.48
    • Extracellular Score: 2.92
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.151
    • Ymax_pos: 27
    • Cmax: 0.153
    • Cmax_pos: 27
    • Smax: 0.293
    • Smax_pos: 1
    • Smean: 0.164
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWEYIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPILCIEMRSDIPLARGLGSSASALVGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGFYNDVSKETSVAHIDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAGKLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVRSLNSQVVSCHSELVDINISGVKERIVYQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]