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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1322 [new locus tag: SA_RS07500 ]
  • pan locus tag?: SAUPAN004024000
  • symbol: srrB
  • pan gene symbol?: srrB
  • synonym:
  • product: respiratory response protein SrrB

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1322 [new locus tag: SA_RS07500 ]
  • symbol: srrB
  • product: respiratory response protein SrrB
  • replicon: chromosome
  • strand: -
  • coordinates: 1530839..1532605
  • length: 1767
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    TTGAGGTTAAATCTAATGATGAGCCGGCTAAATAGTGTCGTAATTAAACTGTGGTTAACT
    ATTATTTTAATAGTGACGACAGTTTTAATTTTATTAAGTATTGCTTTAATTACCTTTATG
    CAATACTATTTCACACAAGAAACCGAAAATGCCATAAGAGAAGATGCTAGACGTATAAGT
    TCACTGGTCGAACAATCACATAATAAAGAAGAAGCAATAAAATATAGTCAAACATTAATT
    GAAAATCCTGGTGGGTTGATGATTATAAATAATAAACATCGTCAATCAACGGCTTCACTT
    TCTAATATTAAAAAGCAAATGTTGAATGAAGTAGTCAACAACGACCATTTTGACGATGTG
    TTTGATAAAGGTAAATCTGTTACTCGAAATGTAACGATTAAAGAAAAGGGTTCATCTCAA
    ACATATATTTTGTTAGGCTATCCAACAAAAGCACAGAAGAATAGTCATAGCAAATATAGT
    GGAGTCTTTATATATAAAGACTTGAAATCAATCGAAGATACAAATAATGCTATTACGATT
    ATCACTATAATTACGGCTGTTATTTTCTTAACAATTACAACAGTCTTTGCGTTTTTCTTA
    TCGTCAAGAATTACAAAACCTTTAAGACGTTTAAGAGACCAAGCTACACGTGTATCTGAA
    GGGGATTACTCTTATAAACCTTCTGTCACAACGAAAGATGAAATTGGTCAATTATCGCAG
    GCATTTAATCAGATGAGTACAGAAATCGAAGAGCATGTCGACGCATTATCCACATCTAAA
    AATATTAGAGACAGCTTAATTAACTCTATGGTAGAAGGTGTCCTAGGTATTAATGAGAGT
    CGACAAATTATCTTATCTAATAAGATGGCGAATGATATTATGGACAATATTGATGAAGAT
    GCTAAAGCTTTCTTATTAAGACAAATTGAAGATACTTTTAAATCAAAACAAACGGAAATG
    CGTGATTTAGAAATGAATGCACGATTCTTTGTTGTGACCACAAGCTATATCGATAAGATT
    GAACAGGGAGGTAAAAGTGGTGTTGTTGTGACAGTTCGTGATATGACTAATGAGCACAAT
    CTAGATCAAATGAAGAAAGATTTCATTGCTAATGTATCACATGAATTACGAACACCTATA
    TCATTACTACAAGGTTATACTGAATCAATTGTAGATGGTATTGTTACAGAACCGGATGAA
    ATAAAAGAATCGCTCGCCGTTGTCCTTGATGAATCGAAACGTTTAAATCGATTAGTTAAT
    GAATTGTTAAATGTCGCACGCATGGATGCTGAAGGGTTATCCGTAAATAAAGAAGTTCAG
    CCTATTGCAGCGTTACTAGATAAGATGAAAATTAAGTATCGCCAACAAGCTGATGATTTA
    GGTCTAAATATGACTTTTAATTATTGTAAGAAGCGTGTTTGGAGTTATGATATGGATCGC
    ATGGACCAAGTACTAACGAACTTAATTGATAATGCATCACGTTATACGAAACCTGGAGAT
    GAAATTGCAATTACTTGTGATGAAAATGAAAGCGAAGATATTTTATACATTAAAGATACA
    GGCACAGGCATTGCACCAGAACATTTACAACAAGTATTTGATCGTTTTTATAAAGTTGAT
    GCAGCGAGAACGCGAGGTAAACAAGGTACCGGTTTAGGTTTGTTCATTTGTAAAATGATT
    ATCGAAGAGCATGGTGGTTCCATTGATGTTAAAAGCGAATTAGGAAAAGGCACAACATTT
    ATTATTAAACTACCAAAACCAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1767

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1322 [new locus tag: SA_RS07500 ]
  • symbol: SrrB
  • description: respiratory response protein SrrB
  • length: 588
  • theoretical pI: 5.93902
  • theoretical MW: 66688.9
  • GRAVY: -0.295408

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 242)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 199.6)
    Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 135.4)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 95.8)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.8)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.8)
    MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.7)
    Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.7)
  • TheSEED:  
    Potassium metabolismPotassium metabolism - no subcategoryPotassium homeostasis Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) 
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 95.1)
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 65.3)
    no clan definedHAMP; HAMP domain (PF00672; HMM-score: 62.8)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 23.4)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.8)
    no clan definedHalogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 18.6)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 16.7)
    no clan definedCep57_MT_bd; Centrosome microtubule-binding domain of Cep57 (PF06657; HMM-score: 14.5)
    EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 13.5)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 12.5)
    DUF3328; Domain of unknown function (DUF3328) (PF11807; HMM-score: 12.3)
    Vpu; Vpu protein (PF00558; HMM-score: 12.2)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 10.8)
    no clan definedSerinc; Serine incorporator (Serinc) (PF03348; HMM-score: 9.4)
    PspB; Phage shock protein B (PF06667; HMM-score: 7.9)
    CemA; CemA family (PF03040; HMM-score: 7.1)
    DUF3165; Protein of unknown function (DUF3165) (PF11364; HMM-score: 6.6)
    TRP; Transient receptor potential (TRP) ion channel (PF06011; HMM-score: 5.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.224
    • Ymax_pos: 12
    • Cmax: 0.157
    • Cmax_pos: 46
    • Smax: 0.585
    • Smax_pos: 5
    • Smean: 0.511
    • D: 0.336
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRLNLMMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETENAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQMLNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIYKDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDYSYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESRQIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDKIEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTEPDEIKESLAVVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQQADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESEDILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVKSELGKGTTFIIKLPKPE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SA1851 (Rex*)
    Rex* (TF) important in Energy metabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]