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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1338 [new locus tag: SA_RS07580 ]
  • pan locus tag?: SAUPAN004042000
  • symbol: malA
  • pan gene symbol?: malA
  • synonym:
  • product: alpha-D-1,4-glucosidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1338 [new locus tag: SA_RS07580 ]
  • symbol: malA
  • product: alpha-D-1,4-glucosidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1545313..1546962
  • length: 1650
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATAAGCAATGGTGGAAAGAAGCAGTAGCATATCAAGTATATCCAAGAAGTTTTAAT
    GATAGTAATCACGATGGTATTGGGGATTTACCTGGAATGATTGATAAATTGGACTACTTA
    AAAGATTTAGGTATCGATGTCATTTGGCTCAGTCCAATGTTTAAATCACCTAATGATGAC
    AATGGTTATGATATTAGTGACTATCAAGAAATTATGGATGAATTTGGAACGATGGAAGAC
    TTTGATTGTTTATTAAAAGGTATTCATGATAGAGGCATGAAGCTTATTTTAGATTTAGTT
    GTAAATCATACATCTGATGAACATCCTTGGTTTATAGAATCTAAATCTAGTAAAGACAAT
    CCCAAACGTGATTGGTATATTTGGCAAGATCCAAAGCAAGATGGCTCTGAACCTAACAAC
    TGGGAAAGTATCTTTAATGGATCTACATGGGAATATGATGCTAATACTGAGCAATATTAT
    TTCCATTTATTCAGTAAAAAACAACCTGATTTGAATTGGGGTAATCCAGAAGTTAGAGAT
    GCTGTATTTGAAATGATGAACTGGTGGTTTGATAAAGGCATTGATGGATTTAGAGTAGAT
    GCAATTACGCATATTAAGAAGACGTTTGAAGCGGGTGACTTACCTGTACCTGAGGGTAAA
    ACATATGCCCCAGCATTTGATGTAGATATGAATCAGCCAGGTATACAAACTTGGTTACAA
    GAGATGAAAGATCGCTCATTAAGTAAGTATGACATTATGACTGTCGGTGAAGCGAATGGT
    GTAAGCCCTGATGATGCTGATGACTGGGTCGGGGAAGAAAATGGTAAATTTAATATGATA
    TTCCAATTTGAACATTTGGGACTGTGGAATAGTGGTGATTCTCACTTTGATGTAAATTCG
    TATAAATCTGTATTAAATAGATGGCAAAAACAACTTGAAAATAAAGGTTGGAATGCGTTG
    TTTATTGAAAATCACGACCAACCACGACGTGTATCGACGTGGGGTGACGATGACAAGTAT
    TGGTATGAATCAGCAACAAGTCATGCAGCTGTTTATTTCTTGCAACAAGGTACGCCATTC
    ATTTATCAAGGTCAAGAAATTGGTATGACGAATTATCCATTTGAAAGTATTGAAACGTTT
    AACGATGTTGCTGTAAAAAATGACTATCAAATAGTGAAAGCTCAAGGTGGAGATGTAGAC
    GCTTTACTTGCGAAATATAAAGATGAGAACCGAGATAATTCTCGCACACCAATGCAATGG
    GATGATACGTTAAATGGAGGATTTACAAATGGTGAACCGTGGTTCCCAGTGAATCTGAAT
    TATAAAACTATCAATGTTGCACAACAATTAGAAGATGAGCATTCAGTATTACAATTTTAT
    AAAGATTTAATTCAATTAAGAAAGTCTAATGATGTATACGTATATGGTCAATTTGATTTA
    GTAGATGCTGAAAATTCACAAGTTTTTGCGTACACGAGAACATTAAATGAAAAGCAAGTT
    CTTATTGTAGGTAATCTTACTAACCACGAAGCAGAATTAACTGTACCATTTGATTTAAGC
    CATGGAGAAGTAAAGCTATTTAATTATGATGCCAAAGTTAATTTAAAACAGTTACGTCCA
    TATGAAGCATGTGTTATCGAACTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1650

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1338 [new locus tag: SA_RS07580 ]
  • symbol: MalA
  • description: alpha-D-1,4-glucosidase
  • length: 549
  • theoretical pI: 4.28392
  • theoretical MW: 63879.2
  • GRAVY: -0.714572

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 597.5)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidestrehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 349.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 66.4)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 63.1)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharides1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 29.9)
    pullulanase, extracellular (TIGR02102; HMM-score: 27.3)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 23.8)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 11.6)
  • TheSEED:  
    CarbohydratesDi- and oligosaccharidesMaltose and Maltodextrin Utilization Alpha-glucosidase (EC 3.2.1.20) 
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 382.7)
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 34.7)
    Glyco_hydro_tim (CL0058) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 16.8)
    GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.5)
    DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 63
    • Cmax: 0.113
    • Cmax_pos: 59
    • Smax: 0.123
    • Smax_pos: 63
    • Smean: 0.076
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15927088 NCBI
  • RefSeq: NP_374621 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLGIDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGIHDRGMKLILDLVVNHTSDEHPWFIESKSSKDNPKRDWYIWQDPKQDGSEPNNWESIFNGSTWEYDANTEQYYFHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPEGKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGKFNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTWGDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIVKAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNLNYKTINVAQQLEDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTNHEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, MalR
    CcpA (TF) important in Carbon catabolism  RegPrecise
    MalR (TF) important in Maltodextrin utilization, Maltose utilization  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]