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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1510 [new locus tag: SA_RS08505 ]
  • pan locus tag?: SAUPAN004309000
  • symbol: gapB
  • pan gene symbol?: gapB
  • synonym:
  • product: glyceraldehyde 3-phosphate dehydrogenase 2

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1510 [new locus tag: SA_RS08505 ]
  • symbol: gapB
  • product: glyceraldehyde 3-phosphate dehydrogenase 2
  • replicon: chromosome
  • strand: -
  • coordinates: 1719375..1720400
  • length: 1026
  • essential: yes DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCAACGAATATTGCAATTAATGGTATGGGTAGAATTGGAAGAATGGTATTACGTATT
    GCATTACAAAATAAAAATTTAAATGTAGTAGCGATAAATGCTAGTTATCCACCCGAAACA
    ATTGCACATTTAATCAATTACGATACAACACATGGAAAATATAATCTAAAAGTTGAACCG
    ATTGAAAATGGATTGCAAGTTGGTGATCATAAAATTAAATTGGTTGCTGATCGCAATCCT
    GAAAACTTGCCATGGAAAGAATTAGATATCGATATTGCTATAGATGCAACTGGTAAATTT
    AATCATGGTGATAAAGCCATCGCACATATTAAAGCAGGTGCCAAAAAAGTATTGTTAACT
    GGTCCTTCAAAAGGTGGACATGTTCAAATGGTAGTTAAAGGCGTAAATGATAACCAATTA
    GATATAGAAGCATTTGACATTTTTAGTAATGCTTCATGTACTACTAATTGCATTGGTCCA
    GTTGCAAAAGTTTTAAATAATCAGTTTGGAATAGTTAATGGTTTAATGACTACTGTTCAC
    GCTATTACAAATGACCAAAAAAATATTGATAATCCACATAAAGATTTAAGACGTGCACGT
    TCATGTAATGAAAGCATTATTCCTACTTCTACTGGTGCGGCGAAAGCTTTAAAAGAAGTA
    TTACCAGAATTAGAAGGTAAATTACACGGCATGGCATTACGTGTACCAACAAAGAATGTA
    TCGCTCGTTGATTTAGTTGTTGATTTAGAAAAAGAAGTAACTGCAGAAGAAGTAAACCAA
    GCTTTTGAAAATGCAGGTTTAGAAGGTATCATAGAAGTCGAACATCAACCACTAGTGTCT
    GTTGATTTTAATACTAATCCAAATTCAGCTATTATTGATGCAAAATCAACAATGGTCATG
    TCAGGGAATAAAGTAAAAGTTATTGCTTGGTATGATAATGAATGGGGTTATTCAAATAGA
    GTTGTAGATGTTGCTGAGCAAATTGGAGCACTTTTGACATCAAAAGAAACTGTAAGTGCA
    AGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1026

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1510 [new locus tag: SA_RS08505 ]
  • symbol: GapB
  • description: glyceraldehyde 3-phosphate dehydrogenase 2
  • length: 341
  • theoretical pI: 6.35212
  • theoretical MW: 36979
  • GRAVY: -0.1739

Function[edit | edit source]

  • reaction:
    EC 1.2.1.12?  ExPASy
    Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 412.6)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 317.7)
    glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 18.2)
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 16.3)
  • TheSEED  :
    • Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (EC 1.2.1.13)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13)
    and 1 more
    Stress Response Oxidative stress Redox-dependent regulation of nucleus processes  NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13)
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 217.2)
    and 4 more
    NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 121.3)
    DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 16)
    KOW (CL0107) Ribosomal_L14e; Ribosomal protein L14 (PF01929; HMM-score: 13.4)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 12.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.67
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.056954
    • TAT(Tat/SPI): 0.000955
    • LIPO(Sec/SPII): 0.002552
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHLINYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNHGDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGPVAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALKEVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQPLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTSKETVSAS

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA1959(glmS)glucosamine--fructose-6-phosphate aminotransferase  [1] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA0802hypothetical protein  [1] (data from MRSA252)
    SA1532hypothetical protein  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)