From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1836 [new locus tag: SA_RS10535 ]
  • pan locus tag?: SAUPAN005241000
  • symbol: groEL
  • pan gene symbol?: groEL
  • synonym:
  • product: molecular chaperone GroEL

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1836 [new locus tag: SA_RS10535 ]
  • symbol: groEL
  • product: molecular chaperone GroEL
  • replicon: chromosome
  • strand: -
  • coordinates: 2072341..2073957
  • length: 1617
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGTTAAACAATTGAAATTCTCTGAAGATGCACGTCAAGCAATGTTACGTGGTGTAGAC
    CAATTAGCAAATGCAGTTAAAGTAACGATTGGTCCTAAAGGACGTAATGTTGTATTAGAT
    AAAGAGTTTACAGCACCTTTAATTACGAACGATGGTGTAACGATTGCTAAAGAAATCGAA
    TTAGAAGATCCATATGAAAATATGGGGGCTAAACTAGTTCAAGAAGTTGCAAATAAGACA
    AATGAAATTGCTGGTGACGGTACGACAACTGCAACAGTATTAGCTCAAGCAATGATTCAA
    GAAGGCTTGAAAAATGTTACAAGTGGTGCGAACCCAGTTGGTTTACGTCAAGGTATCGAC
    AAAGCAGTTAAAGTTGCTGTAGAAGCATTACATGAAAATTCTCAAAAAGTTGAAAATAAA
    AATGAAATAGCGCAAGTAGGTGCGATTTCAGCAGCAGATGAAGAAATTGGACGTTATATT
    TCTGAAGCTATGGAAAAAGTAGGTAACGATGGTGTCATTACAATTGAAGAATCAAATGGA
    CTAAACACTGAACTAGAAGTGGTTGAAGGTATGCAATTTGATCGTGGTTATCAATCACCA
    TATATGGTTACTGATTCAGATAAAATGGTTGCTGAATTAGAACGCCCATACATTTTAGTA
    ACAGATAAGAAAATCTCGTCTTTCCAAGATATCTTACCTTTATTAGAACAAGTGGTTCAA
    TCTAATCGTCCAATCTTAATTGTAGCTGATGAAGTTGAAGGCGATGCATTAACAAATATC
    GTGCTAAACCGAATGCGTGGCACATTTACAGCTGTTGCAGTAAAGGCGCCTGGTTTCGGT
    GATCGTCGTAAAGCAATGTTAGAAGATTTAGCTATTTTAACTGGTGCGCAAGTGATTACT
    GATGATTTAGGCTTAGATTTAAAAGATGCATCAATTGATATGTTAGGTACTGCAAGTAAA
    GTAGAAGTAACTAAAGATAATACCACTGTTGTTGATGGTGACGGTGACGAAAACAGCATT
    GATGCACGTGTAAGCCAATTGAAATCTCAAATTGAAGAAACTGAATCTGACTTTGATCGT
    GAAAAATTACAAGAGCGCTTAGCTAAATTAGCAGGTGGTGTTGCAGTTATTAAAGTAGGT
    GCAGCAAGTGAAACAGAGCTTAAAGAACGTAAATTACGTATTGAAGATGCATTAAATTCT
    ACACGTGCAGCAGTTGAAGAAGGTATTGTTGCAGGTGGTGGTACTGCATTAGTAAATGTT
    TACCAAAAAGTAAGTGAAATTGAAGCAGAAGGTGACATTGAAACAGGTGTAAATATTGTA
    CTTAAAGCACTAACTGCACCAGTTCGTCAAATTGCTGAAAATGCAGGATTAGAAGGTTCT
    GTTATTGTAGAACGTTTGAAAAACGCAGAGCCGGGTGTTGGTTTTAACGCTGCTACAAAC
    GAGTGGGTTAATATGTTAGAAGCAGGTATCGTTGATCCAACTAAAGTAACACGCTCAGCA
    TTACAACATGCTGCAAGTGTTGCAGCAATGTTCTTAACGACTGAAGCGGTTGTAGCATCA
    ATTCCAGAAAAAAATAATGACCAACCTAACATGGGTGGCATGCCGGGAATGATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1617

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1836 [new locus tag: SA_RS10535 ]
  • symbol: GroEL
  • description: molecular chaperone GroEL
  • length: 538
  • theoretical pI: 4.28546
  • theoretical MW: 57571.8
  • GRAVY: -0.1671

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein folding and stabilizationchaperonin GroL (TIGR02348; HMM-score: 825.1)
    Genetic information processingProtein fateProtein folding and stabilizationthermosome, various subunits, archaeal (TIGR02339; HMM-score: 100.4)
    T-complex protein 1, delta subunit (TIGR02342; HMM-score: 89.4)
    T-complex protein 1, eta subunit (TIGR02345; HMM-score: 79.3)
    T-complex protein 1, epsilon subunit (TIGR02343; HMM-score: 74.7)
    T-complex protein 1, zeta subunit (TIGR02347; HMM-score: 71.2)
    T-complex protein 1, beta subunit (TIGR02341; HMM-score: 70.3)
    T-complex protein 1, gamma subunit (TIGR02344; HMM-score: 63.9)
    T-complex protein 1, theta subunit (TIGR02346; HMM-score: 59.7)
    T-complex protein 1, alpha subunit (TIGR02340; HMM-score: 50.1)
    L-seryl-tRNA(Sec) kinase (TIGR03575; EC 2.7.1.164; HMM-score: 15)
  • TheSEED:  
    Phages, Prophages, Transposable elements, PlasmidsPathogenicity islandsStaphylococcal pathogenicity islands SaPI Heat shock protein 60 family chaperone GroEL 
    Protein MetabolismProtein foldingGroEL GroES Heat shock protein 60 family chaperone GroEL 
  • PFAM:
    no clan definedCpn60_TCP1; TCP-1/cpn60 chaperonin family (PF00118; HMM-score: 334.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 26
    • Cmax: 0.137
    • Cmax_pos: 57
    • Smax: 0.192
    • Smax_pos: 2
    • Smean: 0.156
    • D: 0.141
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTAPLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYISEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVAAMFLTTEAVVASIPEKNNDQPNMGGMPGMM

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CtsR, HrcA
    CtsR (TF) important in Heat shock responseRegPrecise
    HrcA (TF) important in Heat shock responseRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)