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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02254
  • pan locus tag?: SAUPAN005241000
  • symbol: groEL
  • pan gene symbol?: groEL
  • synonym:
  • product: chaperonin GroEL

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02254
  • symbol: groEL
  • product: chaperonin GroEL
  • replicon: chromosome
  • strand: -
  • coordinates: 2086760..2088376
  • length: 1617
  • essential: yes [1] DEG other strains
  • comment: The sequence of SAOUHSC_02254 was corrected based on the resequencing performed by Berscheid et al., 2012 [2].

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGTTAAACAATTGAAATTCTCTGAAGATGCACGTCAAGCAATGTTACGTGGTGTTGAC
    CAACTTGCAAATGCAGTTAAAGTAACGATTGGTCCTAAAGGACGTAATGTTGTATTAGAT
    AAAGAGTTTACAGCACCTTTAATTACGAATGATGGTGTGACGATTGCTAAAGAAATCGAA
    TTAGAAGATCCATATGAAAATATGGGGGCTAAACTAGTTCAAGAAGTCGCAAATAAGACA
    AATGAAATTGCTGGTGACGGTACGACAACTGCAACAGTATTAGCTCAAGCAATGATTCAA
    GAAGGCTTGAAAAATGTTACAAGTGGTGCGAACCCAGTTGGTTTACGACAAGGTATCGAC
    AAAGCAGTTAAAGTTGCTGTTGAAGCGTTACATGAAAATTCTCAAAAAGTTGAAAATAAA
    AATGAAATTGCGCAAGTAGGTGCGATTTCAGCAGCAGATGAAGAAATTGGACGTTATATT
    TCTGAAGCTATGGAAAAAGTAGGTAACGATGGTGTCATTACAATTGAAGAATCAAATGGA
    CTAAACACTGAACTAGAAGTGGTTGAAGGTATGCAATTTGATCGTGGTTATCAATCACCG
    TATATGGTTACTGATTCAGATAAAATGGTTGCTGAATTAGAACGCCCATACATTTTAGTA
    ACAGATAAGAAAATCTCGTCTTTCCAAGATATCTTACCTTTATTAGAACAAGTGGTTCAA
    TCTAATCGTCCAATCTTAATTGTAGCTGATGAAGTTGAAGGCGATGCATTAACAAATATC
    GTGCTAAACCGTATGCGTGGCACATTTACAGCTGTTGCAGTAAAAGCACCTGGTTTTGGT
    GATCGTAGAAAAGCGATGCTTGAAGATTTAGCTATTTTAACTGGTGCGCAAGTGATTACT
    GATGATTTAGGCTTAGATTTAAAAGATGCATCAATTGATATGTTAGGTACTGCAAGTAAA
    GTAGAAGTAACTAAAGATAATACCACTGTTGTTGATGGTGACGGTGACGAAAACAGCATT
    GATGCACGTGTTAGCCAATTGAAATCTCAAATTGAAGAAACTGAATCTGACTTTGATCGT
    GAAAAATTACAAGAGCGCTTAGCTAAATTAGCAGGTGGTGTTGCAGTTATCAAAGTAGGT
    GCAGCAAGTGAAACAGAGCTTAAAGAACGTAAATTACGTATTGAAGATGCATTAAATTCT
    ACACGTGCAGCAGTTGAAGAAGGTATTGTTGCAGGTGGTGGTACTGCATTAGTAAATGTT
    TACCAAAAAGTAAGTGAAATTGAAGCTGAAGGTGACATTGAAACAGGTGTAAATATTGTA
    CTTAAAGCATTAACTGCACCAGTTCGTCAAATTGCTGAAAATGCAGGATTAGAAGGTTCT
    GTTATTGTAGAACGTTTGAAAAACGCAGAGCCGGGTGTTGGTTTTAACGCTGCTACAAAC
    GAGTGGGTTAATATGTTAGAAGAAGGTATCGTTGATCCAACTAAAGTAACACGCTCAGCA
    TTACAACATGCTGCAAGTGTTGCAGCAATGTTCTTAACGACTGAAGCGGTTGTAGCATCA
    ATTCCAGAAAAAAATAATGACCAACCTAACATGGGTGGCATGCCAGGAATGATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1617

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02254
  • symbol: GroEL
  • description: chaperonin GroEL
  • length: 538
  • theoretical pI: 4.27242
  • theoretical MW: 57629.9
  • GRAVY: -0.176952

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein folding and stabilizationchaperonin GroL (TIGR02348; HMM-score: 823.7)
    Genetic information processingProtein fateProtein folding and stabilizationthermosome, various subunits, archaeal (TIGR02339; HMM-score: 100.6)
    T-complex protein 1, delta subunit (TIGR02342; HMM-score: 92.7)
    T-complex protein 1, eta subunit (TIGR02345; HMM-score: 79.1)
    T-complex protein 1, epsilon subunit (TIGR02343; HMM-score: 75.1)
    T-complex protein 1, zeta subunit (TIGR02347; HMM-score: 72.1)
    T-complex protein 1, beta subunit (TIGR02341; HMM-score: 70.6)
    T-complex protein 1, gamma subunit (TIGR02344; HMM-score: 63.8)
    T-complex protein 1, theta subunit (TIGR02346; HMM-score: 60.3)
    T-complex protein 1, alpha subunit (TIGR02340; HMM-score: 50.1)
    L-seryl-tRNA(Sec) kinase (TIGR03575; EC 2.7.1.164; HMM-score: 15)
  • TheSEED:  
    Phages, Prophages, Transposable elements, PlasmidsPathogenicity islandsStaphylococcal pathogenicity islands SaPI Heat shock protein 60 family chaperone GroEL 
    Protein MetabolismProtein foldingGroEL GroES Heat shock protein 60 family chaperone GroEL 
  • PFAM:
    no clan definedCpn60_TCP1; TCP-1/cpn60 chaperonin family (PF00118; HMM-score: 334.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_00530elongation factor Tu  [3] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.132
    • Ymax_pos: 26
    • Cmax: 0.137
    • Cmax_pos: 57
    • Smax: 0.192
    • Smax_pos: 2
    • Smean: 0.156
    • D: 0.141
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTAPLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYISEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVAAMFLTTEAVVASIPEKNNDQPNMGGMPGMM

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01685 (HrcA*)
    HrcA* (TF) important in Heat shock response  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int. J. Med. Microbiol.: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  3. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  5. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  6. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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