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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1848 [new locus tag: SA_RS10610 ]
  • pan locus tag?: SAUPAN005287000
  • symbol: nrgA
  • pan gene symbol?: nrgA
  • synonym:
  • product: ammonium transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1848 [new locus tag: SA_RS10610 ]
  • symbol: nrgA
  • product: ammonium transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2086294..2087544
  • length: 1251
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACA
    CCAGGATTAAGTTTATTTTATGGTGGGTTAGTTCAATCTAAAAATGCGCTTAATACTGTC
    ATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTATGGATAACAGTTGGTTTTACA
    ATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
    GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTA
    TTTCAAATGATGTTTTGTACGATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAA
    ATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGACTGCTCTTGTATACAGTCCA
    GTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAATAAACTCGGTGTATTAGATTTCGCT
    GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATT
    GGAAAAGGAAACAAACATTCTGAATCAACACCACATAATCTTATCATTACGTTGATTGGC
    GGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAGGTAGTGCTTTTACATTTGAT
    AATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
    TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGT
    GCATTAGCAGGATTAGTTGTTATTACTCCTGCAGCAGGATATGTAACATATCTTAGTGCA
    ACAATAATGGCTTTAATAGGAGGTATCTGTTGTTATATTGTCATTAATTACATCAAGGTA
    AAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
    GGTGCTGTTTTAACAGCAGTTTTCCAAAGTAAAAAAGCCAATCCTGACATTGAGAATGGC
    TTTATTTATACTGGTGACATACATATTATACTTGTACAAATATTATGTGTAACAGCAGTT
    GTAATTTTTAGTATCGTCATGACGTTTATTATTGCGAAAGTAATTAAATTAATTACACCA
    TTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
    GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1848 [new locus tag: SA_RS10610 ]
  • symbol: NrgA
  • description: ammonium transporter
  • length: 416
  • theoretical pI: 7.37062
  • theoretical MW: 45478.6
  • GRAVY: 0.852885

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsammonium transporter (TIGR00836; HMM-score: 405.2)
    probable ammonium transporter, marine subtype (TIGR03644; HMM-score: 196.7)
  • TheSEED:  
    Ammonia assimilation Ammonium transporter family 
  • PFAM:
    no clan definedAmmonium_transp; Ammonium Transporter Family (PF00909; HMM-score: 375.8)
    Glycine-zipper (CL0500) Gly-zipper_YMGG; YMGG-like Gly-zipper (PF13441; HMM-score: 7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.188
    • Ymax_pos: 11
    • Cmax: 0.19
    • Cmax_pos: 46
    • Smax: 0.487
    • Smax_pos: 4
    • Smean: 0.378
    • D: 0.262
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

  • GI: 15927618 NCBI
  • RefSeq: NP_375151 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CodY, GlnR
    CodY (TF) important in Amino acid metabolismRegPrecise
    GlnR (TF) important in Nitrogen assimilationRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]