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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02270
  • pan locus tag?: SAUPAN005287000
  • symbol: SAOUHSC_02270
  • pan gene symbol?: nrgA
  • synonym:
  • product: ammonium transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02270
  • symbol: SAOUHSC_02270
  • product: ammonium transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2100721..2101971
  • length: 1251
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACA
    CCAGGATTAAGTTTATTTTATGGTGGGTTAGTTCAATCTAAAAATGCGCTTAATACTGTC
    ATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTATGGATAACAGTTGGTTTTACA
    ATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
    GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTA
    TTTCAAATGATGTTTTGTACGATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAA
    ATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGACTGCTCTTGTATACAGTCCA
    GTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAACAAACTCGGTGTATTAGATTTCGCT
    GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATT
    GGAAAAGGAAACAAACATTCTGAATCAACACCACATAATCTTATCATTACGTTGATTGGC
    GGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAGGTAGTGCTTTTACATTTGAT
    AATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
    TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGT
    GCATTAGCAGGATTAGTTGTCATTACTCCTGCAGCAGGATATGTAACATATCTTAGTGCA
    ACAATAATGGCTTTAATAGGAGGTATCTGTTGTTATATTGTCATTAATTACATCAAGGTA
    AAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
    GGTGCTGTTTTAACAGCAGTTTTCCAAAGTAAAAAAGCCAATCCTGACATTGAGAATGGC
    TTTATTTATACTGGTGACATACATATTATACTTGTACAAATATTATGTGTAACAGCAGTT
    GTAATTTTTAGTATAGTCATGACGTTTATTATTGCGAAAGTAATTAAATTAATTACACCA
    TTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
    GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02270
  • symbol: SAOUHSC_02270
  • description: ammonium transporter
  • length: 416
  • theoretical pI: 7.37062
  • theoretical MW: 45478.6
  • GRAVY: 0.852885

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsammonium transporter (TIGR00836; HMM-score: 405.2)
    probable ammonium transporter, marine subtype (TIGR03644; HMM-score: 196.7)
  • TheSEED:  
    Ammonia assimilation Ammonium transporter family 
  • PFAM:
    no clan definedAmmonium_transp; Ammonium Transporter Family (PF00909; HMM-score: 375.8)
    Glycine-zipper (CL0500) Gly-zipper_YMGG; YMGG-like Gly-zipper (PF13441; HMM-score: 7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.188
    • Ymax_pos: 11
    • Cmax: 0.19
    • Cmax_pos: 46
    • Smax: 0.487
    • Smax_pos: 4
    • Smean: 0.378
    • D: 0.262
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]