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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1923 [new locus tag: SA_RS11055 ]
  • pan locus tag?: SAUPAN005413000
  • symbol: rho
  • pan gene symbol?: rho
  • synonym:
  • product: transcription termination factor Rho

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1923 [new locus tag: SA_RS11055 ]
  • symbol: rho
  • product: transcription termination factor Rho
  • replicon: chromosome
  • strand: -
  • coordinates: 2170710..2172026
  • length: 1317
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTGAAAGAGAACGTACATCTCCTCAGTATGAATCATTCCACGAATTGTACAAGAAC
    TATACTACCAAGGAACTCACTCAAAAAGCTAAAACTCTTAAGTTGACGAACTATAGTAAA
    TTAAATAAAAAAGAACTTGTTATAGCTATTATGGAAGCACAAATGGAAAAAGATGGTAAC
    TATTATATGGAAGGTATCTTAGATGATATACAACCAGATGGTTATGGTTTTTTAAGAACA
    GTGAACTATTCTAAAGGGGAAAAAGATATTTATATATCTGCTAGCCAAATTCGTCGTTTT
    GAAATTAAACGTGGGGATAAAGTAACTGGGAAAGTTAGAAAACCTAAAGATAACGAAAAA
    TATTATGGCTTATTACAAGTTGACTTTGTCAATGACCATAACGCAGAAGAAGTGAAGAAA
    CGTCCGCATTTCCAAGCTTTGACACCACTTTATCCAGATGAGCGTATTAAATTAGAGACA
    GAAACACAAAATTATTCAACGCGCATCATGGATTTAGTAACACCGATTGGTTTAGGTCAA
    CGTGGTTTAATAGTGGCGCCACCTAAAGCAGGTAAAACATCGTTATTAAAAGAAATAGCG
    AATGCAATCAGTACGAACAAACCAGATGCAAAGCTATTTATTTTGTTAGTTGGCGAGCGT
    CCTGAAGAGGTGACAGATTTAGAACGCTCAGTAGAAGCTGCTGAAGTCGTTCATTCAACG
    TTTGACGAACCACCAGAACACCATGTTAAAGTAGCTGAATTATTACTTGAACGTGCAAAG
    CGTTTAGTAGAAATTGGGGAAGATGTCATTATTTTAATGGATTCTATAACGAGATTAGCA
    CGCGCTTATAACTTAGTTATTCCACCAAGTGGTCGTACATTATCAGGTGGTTTAGATCCT
    GCATCTTTACACAAACCAAAAGCATTCTTCGGTGCAGCGAGAAATATTGAAGCGGGTGGA
    AGTTTAACAATACTTGCAACTGCATTAGTTGATACGGGTTCACGTATGGACGATATGATT
    TACGAAGAATTTAAAGGAACAGGTAACATGGAGTTACATTTAGATCGTAAATTGTCTGAA
    CGTCGTATCTTCCCTGCAATTGATATTGGCAGAAGTTCAACGCGTAAAGAAGAATTGTTA
    ATAAGTAAATCTGAATTAGACACATTATGGCAATTAAGAAATCTATTCACTGACTCAACT
    GACTTTACTGAAAGATTTATTCGCAAACTTAAAAGGTCTAAGAATAATGAAGATTTCTTC
    AAGCAGCTACAAAAGTCTGCAGAAGAAAGTACTAAAACGGGTCGACCTATAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1317

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1923 [new locus tag: SA_RS11055 ]
  • symbol: Rho
  • description: transcription termination factor Rho
  • length: 438
  • theoretical pI: 8.53546
  • theoretical MW: 49957.7
  • GRAVY: -0.57968

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingTranscriptionTranscription factorstranscription termination factor Rho (TIGR00767; EC 3.6.4.-; HMM-score: 623)
    Cellular processesCellular processesChemotaxis and motilityflagellar protein export ATPase FliI (TIGR03496; EC 3.6.3.14; HMM-score: 83.8)
    MetabolismEnergy metabolismATP-proton motive force interconversionATPase, FliI/YscN family (TIGR01026; EC 3.6.3.14; HMM-score: 83.7)
    Cellular processesCellular processesPathogenesistype III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 83.1)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 83.1)
    flagellar protein export ATPase FliI (TIGR03498; EC 3.6.3.14; HMM-score: 82.9)
    Cellular processesCellular processesChemotaxis and motilityflagellar protein export ATPase FliI (TIGR03497; EC 3.6.3.14; HMM-score: 73.8)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, beta subunit (TIGR01039; EC 3.6.3.14; HMM-score: 36.8)
    alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 33)
    alternate F1F0 ATPase, F1 subunit beta (TIGR03305; EC 3.6.3.-; HMM-score: 25.3)
    Cellular processesCellular processesSporulation and germinationstage III sporulation protein AA (TIGR02858; HMM-score: 24.2)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, alpha subunit (TIGR00962; EC 3.6.3.14; HMM-score: 19.8)
  • TheSEED:  
    RNA MetabolismTranscriptionTranscription factors bacterial Transcription termination factor Rho 
  • PFAM:
    OB (CL0021) Rho_RNA_bind; Rho termination factor, RNA-binding domain (PF07497; HMM-score: 98.1)
    P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 75.6)
    HeH (CL0306) Rho_N; Rho termination factor, N-terminal domain (PF07498; HMM-score: 43.6)
    P-loop_NTPase (CL0023) NACHT; NACHT domain (PF05729; HMM-score: 25.2)
    OB (CL0021) OB_RNB; Ribonuclease B OB domain (PF08206; HMM-score: 21.2)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16)
    AAA_25; AAA domain (PF13481; HMM-score: 15.5)
    AAA_24; AAA domain (PF13479; HMM-score: 15)
    HTH (CL0123) Mor; Mor transcription activator family (PF08765; HMM-score: 14.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 59
    • Cmax: 0.104
    • Cmax_pos: 42
    • Smax: 0.185
    • Smax_pos: 48
    • Smean: 0.099
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15927695 NCBI
  • RefSeq: NP_375228 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVIAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLETETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

R S Washburn, A Marra, A P Bryant, M Rosenberg, D R Gentry
rho is not essential for viability or virulence in Staphylococcus aureus.
Antimicrob. Agents Chemother.: 2001, 45(4);1099-103
[PubMed:11257021] [WorldCat.org] [DOI] (P p)