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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2003 [new locus tag: SA_RS11535 ]
  • pan locus tag?: SAUPAN005610000
  • symbol: hysA
  • pan gene symbol?: hysA
  • synonym:
  • product: hyaluronate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2003 [new locus tag: SA_RS11535 ]
  • symbol: hysA
  • product: hyaluronate lyase
  • replicon: chromosome
  • strand: +
  • coordinates: 2273593..2276022
  • length: 2430
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACATATAGAATGAAGAAATGGCAAAAATTATCCACCATTACGTTATTAATGGCTGGT
    GTGATTACTTTGAATGGTGGTGAATTCAGAAGTATTGATAAACATCAAATCGCTGTGGCT
    GATACGAATGTTCAAACGCCAGATTATGAAAAGTTGAGGAACACATGGTTAGATGTTAAC
    TACGGTTATGATAAGTATGATGAGAAGAATGACGCAATGAAGAAGAAGTTTGATGCTACG
    GAGAAAGAGGCAGAGAAATTACTCAGTAGCATGAAAACTGAAAGTGGAAGGACTTACTTG
    TGGGATAGTGCAAAAGATTTAGATAACAAGTCTGCGGATATGACTCGTACCTATCGTAAT
    ATTGAGAAAATCGCAGAAGCGATGAGGCATCCTAAAACCACTTTAAATACTGACGAAAAT
    AAGAAGAAAGTGAAAGATGCCCTTGAGTGGCTGCATAAAAATGCATATGGAAAAGAACCA
    GATAATAAAGTAAAAGAATTGACTGAGAATTTTAAAATAACTGATTCATCAAAGAAAAAA
    GCTTTAAATTGGTGGGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATA
    TTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACT
    TTCGCCCCAAAAAGTGATGAAATATTATCTTCTGTAGGAAAAGCTGAACCTGCTAAAGGC
    GGAAATTTAGTAGACATTGCTAAAGTAAAACTTTTAGAAAGTATTATCGAAGAAGATAAA
    GATATGATGAAAAATTCAATAGATTCATTTAATAAAGTCTTCACTTACGTTCAAGATTCT
    GCCACTGATAAAGAACGTAATGGATTTTATAAAGATGGTTCATATATCGACCATAAAGAT
    GTCCCATACACTGGTGCTTATGGCGTTGTACTCTTAGAGGGGATTTCTCAAATGATGCCG
    ATGATAAAAGAAACACCTTTTAATGATAAAACCCAAAATAATACAACCTTAAAATCATGG
    ATTGACGACGGATTTTTACCACTCATTTATAAAGGTGAAATGATGGATTTATCACGTGGT
    AGAGCCATTAGCCGTGAAAATGAAACGAGTCACTCAGCATCTGCAACTGTAATGAAATCA
    TTGTTGAGATTAAGTGATGCCATGGATGATTCAACAAAAGCTAAGTATAAAAAAATTGTC
    AAAACTTCAGTTAAATCTGATTCAAGTTACGGCCAAAATGATACATTAAGCTCTTATTCG
    GATATAAGCAAAATGAAGTCTTTAATGGAAGACAGCACTATTTCTACTAACGGTTTAACA
    CAACAACTTAAAATATATAATGACATGGATCGTGTCACCTATCACAATAAAGACTTAGAC
    TTTGCATTTGGTTTAAGTATGACGTCGAAAAACGTCGCACGTTACGAAAGTATCAACGGA
    GAGAACTTAAAAGGTTGGCACACTGGTGCTGGAATGTCTTATTTATACAATAGCGATGTG
    AAACACTACCGTGATAACTTCTGGGCGACAGCTGATATGAAACGTTTAGCAGGTACAACA
    ACTTTAGAAAATGAAGAACCTAAAGGTACGGATGTTAAAAAGTCGAGTAAAACTTTTGTA
    GGCGGAACAAAATTCGATGACCAACATGCTAGTATCGGAATGGATTTTGAAAATCAGGAC
    AAAACTTTAACTGCCAAAAAATCATATTTCATATTAAACGATAAAATTGTCTTCTTAGGA
    ACTGGCATTAAAAGTACTGATTCATCAAAGAATCCAGTTACAACGATTGAAAATCGCAAA
    GCGAATGGGTATACGTTATATACAGACGATAAACAAACAACCGCTTCCGATAATCAGGGA
    ACCAATTCCGTCTTTTTAGAGTCAACCAATAAACCTAAAAACAATATAGGCTATCATTTT
    TTAAACGAATCGAAAATAACTGTAAAAAAAGAAAGTCATACTGGTAAGTGGAGTGATATA
    AATAAAAGTCAAAAGCAAGACAGTAAAACTAATCAATATTATGAAGTAACTCAAAAGCAT
    TCTAATACAGATAGTAAATATGCATATGTGTTGTATCCAGGCTTATCTAAAGATGACTTT
    AATACAAAAAAAGATAAAGTAACTGTCGTTAAACAAGATGATGACTTCCACGTTGTGAAA
    GATAATGAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATT
    AACAACACTAAAGTCGAAGTTAAAGCCAAAGGAATGTTTATTCTTAAAAACAAAGATGAT
    AACACTTATGAATGTAGCTTCTATAATCCCGAATCTACAAATACCGCTTCAGATATTGAA
    TCTAAAATTTCAATAACTGGATACTCTATTACAAACAAAAATACATCAACTTCTAATGAA
    TCCGGCGTACACTTTGAATTAACTAAATAA
    60
    120
    180
    240
    300
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    420
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    720
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    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2430

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2003 [new locus tag: SA_RS11535 ]
  • symbol: HysA
  • description: hyaluronate lyase
  • length: 809
  • theoretical pI: 8.45289
  • theoretical MW: 91812
  • GRAVY: -0.859703

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Hypothetical proteinsConservedTIGR02680 family protein (TIGR02680; HMM-score: 13.4)
  • TheSEED:  
    Hyaluronate lyase precursor (EC 4.2.2.1) 
  • PFAM:
    Hy-ly_N (CL0372) Lyase_8_N; Polysaccharide lyase family 8, N terminal alpha-helical domain (PF08124; HMM-score: 304.3)
    Gal_mutarotase (CL0103) Lyase_8; Polysaccharide lyase family 8, super-sandwich domain (PF02278; HMM-score: 236.7)
    no clan definedLyase_8_C; Polysaccharide lyase family 8, C-terminal beta-sandwich domain (PF02884; HMM-score: 39)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.261
    • Ymax_pos: 13
    • Cmax: 0.192
    • Cmax_pos: 41
    • Smax: 0.765
    • Smax_pos: 7
    • Smean: 0.682
    • D: 0.425
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15927782 NCBI
  • RefSeq: NP_375315 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTYRMKKWQKLSTITLLMAGVITLNGGEFRSIDKHQIAVADTNVQTPDYEKLRNTWLDVNYGYDKYDEKNDAMKKKFDATEKEAEKLLSSMKTESGRTYLWDSAKDLDNKSADMTRTYRNIEKIAEAMRHPKTTLNTDENKKKVKDALEWLHKNAYGKEPDNKVKELTENFKITDSSKKKALNWWDYEIGTPRSLTNTLILLNDQFSNEEKKKFTAPIKTFAPKSDEILSSVGKAEPAKGGNLVDIAKVKLLESIIEEDKDMMKNSIDSFNKVFTYVQDSATDKERNGFYKDGSYIDHKDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNNTTLKSWIDDGFLPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTKAKYKKIVKTSVKSDSSYGQNDTLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMDRVTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFWATADMKRLAGTTTLENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAKKSYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSVFLESTNKPKNNIGYHFLNESKITVKKESHTGKWSDINKSQKQDSKTNQYYEVTQKHSNTDSKYAYVLYPGLSKDDFNTKKDKVTVVKQDDDFHVVKDNESVWAGVNYSNSTQTFDINNTKVEVKAKGMFILKNKDDNTYECSFYNPESTNTASDIESKISITGYSITNKNTSTSNESGVHFELTK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • hysA no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]