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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2194 [new locus tag: SACOL_RS11545 ]
  • pan locus tag?: SAUPAN005610000
  • symbol: hysA
  • pan gene symbol?: hysA
  • synonym:
  • product: hyaluronate lyase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2194 [new locus tag: SACOL_RS11545 ]
  • symbol: hysA
  • product: hyaluronate lyase
  • replicon: chromosome
  • strand: +
  • coordinates: 2274226..2276649
  • length: 2424
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACATATAGAATAAAGAAATGGCAAAAATTATCCACCATTACGTTATTAATGGCTGGT
    GTGATTACTTTGAATGGTGGTGAATTCAGAAGTGTTGATAAACATCAAATCGCTGTGGCT
    GATACGAATGTTCAAACGCCAGATTATGAAAAATTGAGGAACACATGGCTGGACGTTAAC
    TATGGTTATGATAAGTATGATGAGAATAATCCAGATATGAAGAAGAAGTTTGATGCTACA
    GAGAAAGAGGCGACGAATTTACTCAAGGAAATGAAAACTGAAAGTGGTAGGAAATACTTG
    TGGTCAGGAGCGGAAACCCTTGAAACTAATTCTTCTCATATGACTCGTACCTATCGTAAT
    ATTGAGAAAATCGCAGAAGCGATGAGGAATCCTAAAACCACTTTAAATACTGACGAAAAT
    AAGAAGAAAGTGAAAGATGCCCTTGAGTGGCTGCATAAAAATGCATATGGAAAAGAACCA
    GATAAAAAAGTAAAAGAATTAAGTGAGAATTTTACTAAAACAACTGGCAAGAATACCAAC
    TTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTG
    CTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTC
    GCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGA
    AATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGAT
    ATGATGAAAAAGTCTATAGATTCATTTAATAAAGTCTTCACTTACGTTCAAGATTCTGCC
    ACTGGTAAAGAACGTAATGGATTTTATAAAGATGGCTCTTACATTGATCATCAAGACGTT
    CCATACACTGGTGCTTATGGCGTTGTACTCTTAGAGGGGATTTCTCAAATGATGCCGATG
    ATAAAAGAAACACCTTTTAATGATAAAACCCAAAATGATACAACCTTAAAGTCATGGATT
    GACGACGGATTTATGCCACTCATTTATAAAGGTGAAATGATGGATTTATCACGAGGTAGA
    GCTATCAGTCGTGAAAATGAAACCAGTCACTCAGCATCTGCAACAGTAATGAAATCATTG
    TTGAGATTAAGTGATGCCATGGATGATTCAACAAAAGCTAAGTATAAAAAGATTGTCAAA
    TCTTCAGTAGAGTCAGATTCAAGTTATAAACAAAATGATTATTTAAATTCATATTCGGAC
    ATAGATAAAATGAAGTCTTTAATGACAGATAACAGTATTTCTAAAAACGGATTAACACAA
    CAACTTAAAATATATAATGACATGGATCGTGTCACCTATCATAACAAAGACTTAGACTTT
    GCATTTGGTTTAAGTATGACGTCGAAAAACGTAGCACGCTATGAAAGTATCAACGGAGAG
    AATTTAAAAGGTTGGCACACTGGTGCTGGAATGTCTTATTTATATAACAGCGATGTCAAA
    CACTATCATGATAACTTCTGGGTGACAGCCGATATGAAACGTTTATCAGGTACAACAACT
    TTAGACAATGAAATATTAAAAGATACGGATGATAAAAAGTCGAGTAAAACTTTTGTTGGC
    GGAACAAAAGTTGATGACCAACATGCTAGTATCGGAATGGATTTTGAAAATCAGGACAAA
    ACTTTAACTGCCAAAAAATCATATTTCATATTAAACGATAAAATTGTCTTCTTAGGAACT
    GGCATTAAAAGTACTGATTCATCAAAGAATCCAGTTACAACGATTGAAAATCGCAAAGCG
    AATGGGTATACGTTATATACAGACGATAAACAAACAACCAATTCTGATAATCAGGAAAAC
    AATTCAGTCTTTTTAGAGTCCACAGATACCAAAAAGAACATCGGTTATCATTTTTTAAAC
    AAACCGAAAATAACTGTAAAAAAAGAAAGTCATACTGGTAAGTGGAAAGAAATAAATAAA
    AGTCAAAAGGATACACAAAAAACTGATGAGTATTATGAAGTAACTCAAAAGCATTCTAAT
    TCTGACAATAAATATGGATATGTGTTGTATCCAGGCTTATCTAAAGATGTCTTTAAGACA
    AAAAAAGATGAAGTAACTGTCGTTAAGCAAGAAGATGACTTCCACGTTGTGAAAGATAAT
    GAATCGGTTTGGGCTGGTGTTAATTATAGTAATAGCACTCAAACTTTTGACATTAACAAC
    ACTAAAGTCGAAGTTAAAGCCAAAGGTATGTTTATACTTAAAAAGAAAGATGATAACACT
    TATGAATGTAGCTTCTATAATCCTGAATCTACAAATTCCGCTTCAGATATTGAATCTAAA
    ATTTCAATGACCGGTTACTCTATTACAAACAAAAATACGTCGACTTCTAATGAATCCGGC
    GTGCACTTTGAATTAACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2424

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2194 [new locus tag: SACOL_RS11545 ]
  • symbol: HysA
  • description: hyaluronate lyase
  • length: 807
  • theoretical pI: 7.79135
  • theoretical MW: 91984.3
  • GRAVY: -0.878934

Function[edit source | edit]

  • reaction:
    EC 4.2.2.1?  ExPASy
    Hyaluronate lyaseCleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
  • TIGRFAM:
    Hypothetical proteinsConservedTIGR02680 family protein (TIGR02680; HMM-score: 12.9)
  • TheSEED:  
    Hyaluronate lyase precursor (EC 4.2.2.1) 
  • PFAM:
    Hy-ly_N (CL0372) Lyase_8_N; Polysaccharide lyase family 8, N terminal alpha-helical domain (PF08124; HMM-score: 300.8)
    Gal_mutarotase (CL0103) Lyase_8; Polysaccharide lyase family 8, super-sandwich domain (PF02278; HMM-score: 232)
    no clan definedLyase_8_C; Polysaccharide lyase family 8, C-terminal beta-sandwich domain (PF02884; HMM-score: 37.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.245
    • Ymax_pos: 13
    • Cmax: 0.193
    • Cmax_pos: 41
    • Smax: 0.703
    • Smax_pos: 7
    • Smean: 0.606
    • D: 0.386
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNVQTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWSGAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEPDKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIKTFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYVQDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDTTLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTKAKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDRVTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWVTADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKKSYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVFLESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSDNKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINNTKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNESGVHFELTK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • hysA no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]