NCBI: 26-AUG-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus N315
- locus tag: SA2028 [new locus tag: SA_RS11665 ]
- pan locus tag?: SAUPAN005682000
- symbol: secY
- pan gene symbol?: secY
- synonym:
- product: preprotein translocase subunit SecY
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SA2028 [new locus tag: SA_RS11665 ]
- symbol: secY
- product: preprotein translocase subunit SecY
- replicon: chromosome
- strand: -
- coordinates: 2298151..2299443
- length: 1293
- essential: yes [1] DEG other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 1124948 NCBI
- RefSeq: NP_375343 NCBI
- MicrobesOnline: 104369 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
- 1
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1261ATGATTCAAACCCTTGTGAACTTCTTTAGAACAAAAGAGGTTCGTAACAAGATTTTCTTC
ACACTAGCAATGTTAGTAATTTTTAAAATAGGGACTTATATACCAGCTCCAGGAGTAAAT
CCTGCAGCTTTTGATAATCCCCAAGGTTCTCAAGGTGCCACTGAGTTATTAAATACTTTT
GGTGGCGGAGCCTTGAAACGATTTTCTATTTTTGCAATGGGTATTGTACCCTACATCACT
GCATCAATCGTAATGCAATTATTACAAATGGATATTGTCCCTAAATTCTCAGAATGGGCA
AAACAAGGTGAAGTAGGTAGAAGAAAGTTAAATAACGTTACTCGTTATTTAGCAATTTCT
TTAGCATTTATCCAATCTATAGGTATGGCATTCCAATTTAATAATTATCTCAAAGGTGCG
CTGATTATCAATCAGTCAATTATGAGTTATTTATTAATAGCACTAGTTTTGACAGCAGGA
ACTGCTTTCTTAATATGGCTTGGTGATCAAATCACTCAGTTCGGTGTTGGTAATGGTATT
TCTATTATCATATTTGCGGGTATTTTATCAACGTTGCCAGCCAGTCTAATTCAATTTGGA
CAAACTGCGTTCGTTGGGCAAGAAGATACATCATTAGCATGGTTGAAAGTATTAGGACTT
TTAGTGTCACTGATACTATTAACTGTAGGTGCTATTTATGTACTTGAAGCCGTTCGTAAA
ATTCCGATTCAATATGCTAAGAAACAAACTGCTCAAAGATTAGGTTCACAGGCAACTTAT
CTACCTTTAAAAGTGAACTCAGCCGGTGTTATTCCTGTAATCTTTGCAATGGCGTTCTTC
TTACTGCCTAGAACATTAACGTTGTTCTATCCAGATAAAGAATGGGCACAAAACATTGCG
AATGCTGCAAATCCTTCAAGTAATGTTGGTATGGTTGTATATATTGTACTAATTATACTA
TTCACATACTTCTATGCATTTGTACAAGTTAATCCGGAAAAAATGGCTGATAACCTTAAG
AAACAAGGTAGCTATGTTCCAGGCATTAGACCTGGTGAACAAACCAAAAAATATATTACT
AAAGTTTTATATCGTTTAACTTTTGTAGGTTCTATCTTCTTAGCCGTTATTTCAATTCTA
CCAATATTAGCAACTAAGTTTATGGGATTACCACAATCAATTCAGATTGGTGGTACAAGT
TTACTTATCGTTATTGGTGTAGCGATTGAAACAATGAAATCTTTAGAAGCTCAAGTATCT
CAAAAAGAATATAAAGGCTTTGGTGGTAGATAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SA2028 [new locus tag: SA_RS11665 ]
- symbol: SecY
- description: preprotein translocase subunit SecY
- length: 430
- theoretical pI: 10.3709
- theoretical MW: 47147.6
- GRAVY: 0.554419
⊟Function[edit source | edit]
- TIGRFAM: Protein fateProtein and peptide secretion and traffickingpreprotein translocase, SecY subunit (TIGR00967; HMM-score: 482.9)and 1 moreProtein fateProtein and peptide secretion and traffickingaccessory Sec system translocase SecY2 (TIGR02920; HMM-score: 209.7)
- TheSEED:
- PFAM: no clan definedSecY; SecY translocase (PF00344; HMM-score: 360.2)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 9
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular Possibility: 0.17
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.276
- Ymax_pos: 43
- Cmax: 0.3
- Cmax_pos: 53
- Smax: 0.678
- Smax_pos: 33
- Smean: 0.375
- D: 0.315
- predicted transmembrane helices (TMHMM): 10
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAFDNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQGEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAGTAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVLGLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFAMAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEKMADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQSIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: adk < secY < rplO < rpmD < rpsE < rplR < rplF < rpsH < rpsN < rplE < rplX < rplN < rpsQ < rpmC < rplP < rpsC < rplV < rpsS < rplB < rplW < rplD < rplC < rpsJ
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
- ↑
R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
Mol. Microbiol.: 2002, 43(6);1387-400
[PubMed:11952893] [WorldCat.org] (P p)
⊟Relevant publications[edit source | edit]
