NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2316 [new locus tag: SA_RS13275 ]
• pan locus tag^?: SAUPAN006178000
• symbol: srtA
• pan gene symbol^?: srtA
• synonym:
• product: sortase

⊟Gene

⊟General

• type: CDS
• locus tag: SA2316 [new locus tag: SA_RS13275 ]
• symbol: srtA
• product: sortase
• replicon: chromosome
• strand: -
• coordinates: 2600886..2601506
• length: 621
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125243 NCBI
• RefSeq: NP_375640 NCBI
• MicrobesOnline: 104666 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAATGGACAAATCGATTAATGACAATCGCTGGTGTAGTACTTATCCTAGTGGCA
  GCATATTTGTTTGCTAAACCACATATCGATAATTATCTTCACGATAAAGATAAAGATGAA
  AAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGTAAAGACAATAAGCAGCAA
  GCTAAACCTCAAATTCCGAAAGATAAATCAAAAGTGGCAGGCTATATTGAAATTCCAGAT
  GCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCTGAACAATTAAATAGAGGT
  GTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAATATTTCAATTGCAGGACAC
  ACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAAGCAGCCAAAAAAGGTAGT
  ATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAAATGACAAGTATAAGAGAT
  GTTAAGCCAACAGATGTAGAAGTTCTAGATGAACAAAAAGGTAAAGATAAACAATTAACA
  TTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGGGAAAAACGTAAAATCTTT
  GTAGCTACAGAAGTCAAATAA
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  621
  

⊟Protein

⊟General

• locus tag: SA2316 [new locus tag: SA_RS13275 ]
• symbol: SrtA
• description: sortase
• length: 206
• theoretical pI: 8.80677
• theoretical MW: 23598.7
• GRAVY: -0.80534

⊟Function

• reaction:
• TIGRFAM:
  Cell structureCell envelopeOthersortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)
  Genetic information processingProtein fateProtein and peptide secretion and traffickingsortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)
  sortase, marine proteobacterial type (TIGR03784; HMM-score: 35.6)
  Cell structureCell envelopeOthersortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)
  Genetic information processingProtein fateProtein and peptide secretion and traffickingsortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)
• TheSEED:  
  Cell Wall and CapsuleGram-Positive cell wall componentsSortase LPXTG specific sortase A 
  Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryHeme, hemin uptake and utilization systems in GramPositives LPXTG specific sortase A 
• PFAM:
  no clan definedSortase; Sortase family (PF04203; HMM-score: 76)
  DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 17.2)
  DUF3959; Protein of unknown function (DUF3959) (PF13105; HMM-score: 11.6)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.87
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: 0.5
  • N-terminally Anchored Score: 7
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.301
  • Ymax_pos: 26
  • Cmax: 0.174
  • Cmax_pos: 26
  • Smax: 0.657
  • Smax_pos: 23
  • Smean: 0.554
  • D: 0.4
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15928107 NCBI
• RefSeq: NP_375640 NCBI
• UniProt: A0A0H3JNM2 UniProt

⊟Protein sequence

• MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• srtA no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟Relevant publications

Matthew L Bentley, Helena Gaweska, Joseph M Kielec, Dewey G McCafferty
Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA.
J. Biol. Chem.: 2007, 282(9);6571-81
[PubMed:17200112] [WorldCat.org] [DOI] (P p) Hidehiko Hirakawa, Suguru Ishikawa, Teruyuki Nagamune
Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
Biotechnol. Bioeng.: 2012, 109(12);2955-61
[PubMed:22729808] [WorldCat.org] [DOI] (I p)