NCBI: 26-AUG-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus N315
- locus tag: SA2316 [new locus tag: SA_RS13275 ]
- pan locus tag?: SAUPAN006178000
- symbol: srtA
- pan gene symbol?: srtA
- synonym:
- product: sortase
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SA2316 [new locus tag: SA_RS13275 ]
- symbol: srtA
- product: sortase
- replicon: chromosome
- strand: -
- coordinates: 2600886..2601506
- length: 621
- essential: no DEG other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 1125243 NCBI
- RefSeq: NP_375640 NCBI
- MicrobesOnline: 104666 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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601ATGAAAAAATGGACAAATCGATTAATGACAATCGCTGGTGTAGTACTTATCCTAGTGGCA
GCATATTTGTTTGCTAAACCACATATCGATAATTATCTTCACGATAAAGATAAAGATGAA
AAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGTAAAGACAATAAGCAGCAA
GCTAAACCTCAAATTCCGAAAGATAAATCAAAAGTGGCAGGCTATATTGAAATTCCAGAT
GCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCTGAACAATTAAATAGAGGT
GTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAATATTTCAATTGCAGGACAC
ACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAAGCAGCCAAAAAAGGTAGT
ATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAAATGACAAGTATAAGAGAT
GTTAAGCCAACAGATGTAGAAGTTCTAGATGAACAAAAAGGTAAAGATAAACAATTAACA
TTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGGGAAAAACGTAAAATCTTT
GTAGCTACAGAAGTCAAATAA60
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621
⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SA2316 [new locus tag: SA_RS13275 ]
- symbol: SrtA
- description: sortase
- length: 206
- theoretical pI: 8.80677
- theoretical MW: 23598.7
- GRAVY: -0.80534
⊟Function[edit source | edit]
- reaction:
- TIGRFAM: Cell envelopeOthersortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)Protein fateProtein and peptide secretion and traffickingsortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)sortase, marine proteobacterial type (TIGR03784; HMM-score: 35.6)Cell envelopeOthersortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)Protein fateProtein and peptide secretion and traffickingsortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)
- TheSEED:
- PFAM: no clan definedSortase; Sortase family (PF04203; HMM-score: 76)DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 17.2)DUF3959; Protein of unknown function (DUF3959) (PF13105; HMM-score: 11.6)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 9.87
- Cellwall Score: 0.12
- Extracellular Score: 0.01
- Internal Helix: 1
- LocateP: N-terminally anchored (No CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular Possibility: 0.17
- Signal Peptide Possibility: 0.5
- N-terminally Anchored Score: 7
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.301
- Ymax_pos: 26
- Cmax: 0.174
- Cmax_pos: 26
- Smax: 0.657
- Smax_pos: 23
- Smean: 0.554
- D: 0.4
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- srtA no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]
Matthew L Bentley, Helena Gaweska, Joseph M Kielec, Dewey G McCafferty
Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA.
J. Biol. Chem.: 2007, 282(9);6571-81
[PubMed:17200112] [WorldCat.org] [DOI] (P p)
Hidehiko Hirakawa, Suguru Ishikawa, Teruyuki Nagamune
Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
Biotechnol. Bioeng.: 2012, 109(12);2955-61
[PubMed:22729808] [WorldCat.org] [DOI] (I p)