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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2469 [new locus tag: SA_RS14135 ]
  • pan locus tag?: SAUPAN006432000
  • symbol: SA2469
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2469 [new locus tag: SA_RS14135 ]
  • symbol: SA2469
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2780585..2781598
  • length: 1014
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTTATATTGATAAAAATGAAAGTCCAGTTACGCCGTTGGATGAAAAAACAATGACG
    TCTATTATTAGTGCAACGCCATATAATTTATATCCTGATGCAGCATATGAACAATTCAAG
    GAAGCTTATGCTAAGTTTTACGGATTATCGCCTGAACAAATTATTGCAGGAAATGGATCT
    GATGAATTGATTCAAAAGTTAATGCTGATCATGCCAGAAGGTCCGGCATTAACGCTAAAT
    CCTGATTTTTTTATGTATCAAGCATATGCGGCACAAGTAAATCGTGAAATTGCATTTGTA
    GATGCAGGATCAGATTTAACGTTTGATTTGGAAACCATTTTAACGAAAATCGATGAAGTA
    CAACCATCATTTTTTATTATGAGTAATCCACATAACCCTTCAGGCAAGCAATTTGATACG
    GCATTTTTAACAGCTATTGCAGATAAGATGAAAGCATTAAACGGATACTTTGTCATTGAT
    GAAGCATATTTAGATTATGGTACGGCATATGACGTGGAACTGGCACCACACATCTTAAGA
    ATGCGTACATTATCAAAGGCGTTTGGAATTGCCGGCTTAAGATTAGGTGTCTTAATTAGT
    ACTGCTGGAACGATAAAGCATATTCAAAAAATAGAACATCCATATCCATTAAATGTATTT
    ACGCTAAATATTGCGACTTATATTTTTAGACATAGAGAAGAGACAAGAGTATTTTTAACG
    ATGCAACGACAGTTAGCTGAGCAGTTAAAACAAATATTTGATACACATGTTGCAGATAAA
    ATGTCAGTATTCCCATCAAATGCCAATTTTGTACTTACTAAAGGTTCAGCAGCGCACCAA
    TTAGGACAATACGTATATGAACAAGGATTTAAACCTCGCTTTTATGATGAGCCGGTGATG
    AAGGGCTATGTAAGATACTCAATTGCAACAGCATCGCAGTTAAAGCAATTAGAAGAAATT
    GTTAAAGAATGGAGCGCAAAATATGATTTATCAAAAACAACGAAACACAGCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1014

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2469 [new locus tag: SA_RS14135 ]
  • symbol: SA2469
  • description: hypothetical protein
  • length: 337
  • theoretical pI: 5.87594
  • theoretical MW: 38220.5
  • GRAVY: -0.177448

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismAmino acid biosynthesisHistidine familyhistidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 196.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminthreonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 162.1)
    Cellular processesCellular processesBiosynthesis of natural productscapreomycidine synthase (TIGR03947; HMM-score: 45.9)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 31.8)
    MetabolismEnergy metabolismAmino acids and aminestyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 26.7)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 26.4)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 19.9)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 18.2)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 17.8)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 15)
  • TheSEED:  
    Histidine Biosynthesis in plants Similar to histidinol-phosphate aminotransferase 
    Histidine Biosynthesis Similar to histidinol-phosphate aminotransferase 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 123.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 36
    • Cmax: 0.133
    • Cmax_pos: 36
    • Smax: 0.199
    • Smax_pos: 34
    • Smean: 0.097
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYAKFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDAGSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVIDEAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLNVFTLNIATYIFRHREETRVFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGSAAHQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTTKHS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: SA1723 (HisR*), CodY
    HisR* (TF) important in Histidine biosynthesis  RegPrecise
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]