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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0209 [new locus tag: SACOL_RS01065 ]
  • pan locus tag?: SAUPAN001088000
  • symbol: SACOL0209
  • pan gene symbol?: coa
  • synonym:
  • product: staphylocoagulase precursor

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0209 [new locus tag: SACOL_RS01065 ]
  • symbol: SACOL0209
  • product: staphylocoagulase precursor
  • replicon: chromosome
  • strand: +
  • coordinates: 245645..247555
  • length: 1911
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAAAGCAAATAATTTCGCTAGGCGCATTAGCAGTTGCATCTAGCTTATTTACATGG
    GATAACAAAGCAGATGCGATAGTAACAAAGGATTATAGTGGGAAATCACAAGTTAATGCT
    GGGAGTAAAAATGGGACATTAATAGATAGCAGATATTTAAATTCAGCTCTATATTATTTG
    GAAGACTATATAATTTATGCTATAGGATTAACTAATAAATATGAATATGGAGATAATATT
    TATAAAGAAGCTAAAGATAGGTTGTTGGAAAAGGTATTAAGGGAAGATCAATATCTTTTG
    GAGAGAAAGAAATCTCAATATGAAGATTATAAACAATGGTATGCAAATTATAAAAAAGAA
    AATCCTCGTACAGATTTAAAAATGGCTAATTTTCATAAATATAATTTAGAAGAACTTTCG
    ATGAAAGAATACAATGAACTACAGGATGCATTAAAGAGAGCACTGGATGATTTTCACAGA
    GAAGTTAAAGATATTAAGGATAAGAATTCAGACTTGAAAACTTTTAATGCAGCAGAAGAA
    GATAAAGCAACTAAGGAAGTATACGATCTCGTATCTGAAATTGATACATTAGTTGTATCA
    TATTATGGTGATAAGGATTATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTA
    ATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACGTATTAAAAAAGAAATG
    ATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCG
    AAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAA
    CCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCT
    TGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAA
    GAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATAACCAACAAGAGGTTAAA
    ACTACGGCTGGTAAAGCTGAAGAAACAACACAACCAGTTGCACAACCATTAGTTAAAATT
    CCACAGGGCACAATTACAGGTGAAATTGTAAAAGGTCCGGAATATCCAACGATGGAAAAT
    AAAACGGTACAAGGTGAAATCGTTCAAGGTCCCGATTTTCTAACAATGGAACAAAGCGGC
    CCATCATTAAGCAATAATTATACAAACCCACCGTTAACGAACCCTATTTTAGAAGGTCTT
    GAAGGTAGCTCATCTAAACTTGAAATAAAACCACAAGGTACTGAATCAACGTTAAAAGGT
    ACTCAAGGAGAATCAAGTGATATTGAAGTTAAACCTCAAGCAACTGAAACAACAGAAGCT
    TCTCAATATGGTCCGAGACCGCAATTTAACAAAACACCTAAATATGTTAAATATAGAGAT
    GCTGGTACAGGTATCCGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGA
    TTCAATAAGCCATCAGAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTA
    TCATACGGAGCTCGTCCGACATACAAGAAGCCAAGCGAAACGAATGCATACAATGTAACA
    ACACATGCAAACGGCCAAGTATCATACGGAGCTCGTCCGACACAAAACAAGCCAAGCAAA
    ACAAACGCATATAACGTAACAACACATGGAAACGGCCAAGTATCATATGGCGCTCGCCCA
    ACACAAAACAAGCCAAGCAAAACAAATGCATACAACGTAACAACACATGCAAACGGTCAA
    GTGTCATACGGAGCTCGCCCGACATACAAGAAGCCAAGTAAAACAAATGCATACAATGTA
    ACAACACATGCAGATGGTACTGCGACATATGGGCCTAGAGTAACAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1911

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0209 [new locus tag: SACOL_RS01065 ]
  • symbol: SACOL0209
  • description: staphylocoagulase precursor
  • length: 636
  • theoretical pI: 8.89619
  • theoretical MW: 71717.1
  • GRAVY: -1.04497

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    two transmembrane protein (TIGR04527; HMM-score: 16.8)
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Staphylocoagulase 
  • PFAM:
    no clan definedCoagulase; Staphylococcus aureus coagulase (PF08764; HMM-score: 451.7)
    Staphylcoagulse; Staphylocoagulase repeat (PF04022; HMM-score: 281.7)
    Ntox25; Bacterial toxin 25 (PF15530; HMM-score: 13.8)
    Zn_Beta_Ribbon (CL0167) DUF1451; Zinc-ribbon containing domain (PF07295; HMM-score: 9.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NKADAIVT
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.642
    • Ymax_pos: 27
    • Cmax: 0.742
    • Cmax_pos: 27
    • Smax: 0.739
    • Smax_pos: 2
    • Smean: 0.592
    • D: 0.622
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKNGTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLERKKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHREVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKLDLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTNSDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVDNQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPDFLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR
    SaeR (TF) important in Virulence  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]