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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0343 [new locus tag: SACOL_RS01725 ]
  • pan locus tag?: SAUPAN001446000
  • symbol: SACOL0343
  • pan gene symbol?:
  • synonym:
  • product: prophage L54a, replicative DNA helicase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0343 [new locus tag: SACOL_RS01725 ]
  • symbol: SACOL0343
  • product: prophage L54a, replicative DNA helicase
  • replicon: chromosome
  • strand: +
  • coordinates: 365904..367145
  • length: 1242
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATTATGGACAATTTGAAATTGAAAGCACAATAATCGCTACGCTGCTCAAACAACCG
    GACGTACTAGAAAAGATACGAGTTAAAGATTATATGTTTACGAACGAAAAGTTTAAAACC
    TTTTTCAATTATGTAATGGACGTCGGAAAGATAGATCATCAAGAAATTTATTTAAAAGCA
    ACTAAAGATAAAGAATTTTTAGATGCAGATACTATAACTAAACTTTATAACTCCGATTTC
    ATTGGATACGGTTTCTTTGAACGTTATCAACAAGAATTATTGGAAAGTTATCAACTCAAC
    AAAGCTAACGAATTGGTAACTGAGTTCAAACAACAACCTACGAATCAAAACTTTAACAAC
    TTGATTGATGAACTCAAGGATTTAAAAACGATTACTAACAAAAAAGAAGATGGAACCAAG
    AAGTTTGTTGAGGAGTTTGTCGAAGAGTTATACAGCGATAGCCCTAAGAAGCAAATTAAG
    ACGGGTTACAAGCTAATGGATTACAAAATAGGGGGATTAGAGCCATCACAATTAATCGTC
    ATCGCAGCGCGTCCCTCAGTGGGTAAGACAGGCTTTGCATTAAACATGATGCTGAACATA
    GCACAAAATGGATACAAAACATCTTTCTTTAGTCTCGAAACAACCGGCACATCGGTATTG
    AAACGTATGTTATCAACAATTACTGGTATTGAGTTAACAAAGATAAAAGAAATCAGGAAC
    TTAACGCCGGATGACTTAACAAAGTTAACGAATGCGATGGATAAAATCATGAAATTAGGC
    ATCGATATTTCTGATAAAAGTAATATCACACCGCAAGATGTGCGAGCGCAAGCAATGAGG
    CATTCAGACGGGCAACAAGTTATTTTTATAGATTACCTTCAACTGATGGATACTGATGCG
    AAAGTTGATAGACGTGTAGCAGTAGAAAAGATATCACGCGACTTAAAGATAATCGCTAAC
    GAGACAGGCGCAATCATTGTACTACTTTCACAACTGAATCGTGGAGTCGAGTCTAGGCAG
    GATAAACGACCAATGCTATCGGACATGAAAGAATCAGGCGGAATAGAGGCAGATGCGAGT
    TTAGCAATGCTACTTTACCGTGATGATTATTATAACCGTGACGAAGATGACAGTATCACA
    GGCAAATCTATTGTTGAATGTAACATAGCCAAAAACAAAGACGGCGAAACCGGAATAATT
    GAATTCGAGTATTACAAGAAGACGCAGAGGTTTTTCACATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1242

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0343 [new locus tag: SACOL_RS01725 ]
  • symbol: SACOL0343
  • description: prophage L54a, replicative DNA helicase
  • length: 413
  • theoretical pI: 5.0803
  • theoretical MW: 47460.9
  • GRAVY: -0.492736

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairreplicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 272.6)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage replicative helicase, DnaB family (TIGR03600; HMM-score: 235.4)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 19.2)
    KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 19.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA repair protein RadA (TIGR00416; HMM-score: 15.8)
  • TheSEED:  
    DNA helicase, phage-associated 
  • PFAM:
    P-loop_NTPase (CL0023) DnaB_C; DnaB-like helicase C terminal domain (PF03796; HMM-score: 249.9)
    AAA_25; AAA domain (PF13481; HMM-score: 34.8)
    no clan definedDnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 13)
    HAD (CL0137) Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.101
    • Ymax_pos: 29
    • Cmax: 0.117
    • Cmax_pos: 29
    • Smax: 0.129
    • Smax_pos: 1
    • Smean: 0.076
    • D: 0.091
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNYGQFEIESTIIATLLKQPDVLEKIRVKDYMFTNEKFKTFFNYVMDVGKIDHQEIYLKATKDKEFLDADTITKLYNSDFIGYGFFERYQQELLESYQLNKANELVTEFKQQPTNQNFNNLIDELKDLKTITNKKEDGTKKFVEEFVEELYSDSPKKQIKTGYKLMDYKIGGLEPSQLIVIAARPSVGKTGFALNMMLNIAQNGYKTSFFSLETTGTSVLKRMLSTITGIELTKIKEIRNLTPDDLTKLTNAMDKIMKLGIDISDKSNITPQDVRAQAMRHSDGQQVIFIDYLQLMDTDAKVDRRVAVEKISRDLKIIANETGAIIVLLSQLNRGVESRQDKRPMLSDMKESGGIEADASLAMLLYRDDYYNRDEDDSITGKSIVECNIAKNKDGETGIIEFEYYKKTQRFFT

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]