From AureoWiki
Jump to: navigation, search

NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0449 [new locus tag: SACOL_RS02270 ]
  • pan locus tag?: SAUPAN001978000
  • symbol: SACOL0449
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0449 [new locus tag: SACOL_RS02270 ]
  • symbol: SACOL0449
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 450722..451348
  • length: 627
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAAAAGAGATTACTACTAAGTACATTTTTAGCATCGACATTAATTCTTACAGGTTGC
    GCGTCCGATCAATCTGATAACGAAGATCATCATACAAGTACTGGCATCCATGCACCTAAA
    AGCGCTAAAAAATTAGAAACAAAAGATATCTTTAACTCAGATAAGAAAAATAGTGATATT
    AGTGATGCAGAGATGAAGCAAGCTATAGAAAAGTATTTGTCTGTAAATAGCGATATACTT
    GATAACAAATATATTATGCAACATAAACTAGATAAACAAATTGACAGTCAAACAAAAGTG
    ACTGAAAAACAAGCAGAAACACTTAGCCACTTATCTAACTTAGCTGTTAAAAACGATTTG
    CATTTCAAGAAATTTGTCACTGAAAACAATATTCCTAAAGAATATAAGAAACCCGTTGAA
    TTAATGATGAATTACTTCAAAGCTTTAAATAGTACAATCGCCAATGTTGATGAAGATATT
    GAAAAGCTTAGCTATCAACCGCAAAATAAAATCAATGTTGTCGACGTGCCAACAAAATAT
    GCCGGTGATGTTAATAAAAAGCAACAAGATAAAATTAAAGATTTCTTAAAATCTAAAGGT
    ATCAAGAGTGATGTTATCGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0449 [new locus tag: SACOL_RS02270 ]
  • symbol: SACOL0449
  • description: hypothetical protein
  • length: 208
  • theoretical pI: 9.1737
  • theoretical MW: 23663.8
  • GRAVY: -0.759135

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    extracellular lipase, Pla-1/cef family (TIGR03502; EC 3.1.-.-; HMM-score: 18.9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingouter membrane assembly lipoprotein YfiO (TIGR03302; HMM-score: 18.7)
    and 1 more
    Cellular processesCellular processesSporulation and germinationsporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 12.2)
  • TheSEED:  
    FIG01108125: hypothetical protein 
  • PFAM:
    no clan definedDUF1748; Fungal protein of unknown function (DUF1748) (PF08520; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: -0.33
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LILTGCA
  • SignalP: Signal peptide length 22 aa
    • Ymax: 0.467
    • Ymax_pos: 22
    • Cmax: 0.241
    • Cmax_pos: 22
    • Smax: 0.957
    • Smax_pos: 20
    • Smean: 0.915
    • D: 0.642
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKKLETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTEKQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDIEKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Annette Dreisbach, Kristina Hempel, Girbe Buist, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
    Profiling the surfacome of Staphylococcus aureus.
    Proteomics: 2010, 10(17);3082-96
    [PubMed:20662103] [WorldCat.org] [DOI] (I p)
  4. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]