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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0693 [new locus tag: SACOL_RS03565 ]
- pan locus tag?: SAUPAN002512000
- symbol: tagA
- pan gene symbol?: tagA
- synonym:
- product: teichoic acid biosynthesis protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0693 [new locus tag: SACOL_RS03565 ]
- symbol: tagA
- product: teichoic acid biosynthesis protein
- replicon: chromosome
- strand: +
- coordinates: 717314..718078
- length: 765
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236583 NCBI
- RefSeq: YP_185575 NCBI
- BioCyc: see SACOL_RS03565
- MicrobesOnline: 912171 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGACTGTTGAAGAAAGATCCAATACAGCCAAAGTTGACATTTTAGGGGTCGATTTTGAT
AATACAACAATGTTGCAAATGGTTGAAAATATTAAAACCTTTTTTGCAAATCAATCAACG
AATAATCTTTTTATAGTAACAGCCAACCCTGAAATAGTGAATTACGCGACGACACATCAA
GCGTATTTAGAGTTAATAAATCAAGCGAGCTATATTGTTGCTGATGGGACAGGAGTAGTC
AAAGCTTCGCATCGTTTAAAGCAACCTCTAGCGCATCGTATACCTGGTATTGAGTTGATG
GATGAATGTTTGAAAATTGCTCATGTAAATCATCAAAAAGTATTTTTGCTAGGGGCAACT
AATGAAGTTGTAGAAGCGGCACAATATGCATTGCAACAAAGATATCCAAACATATCGTTT
GCACATCATCACGGTTATATTGATTTAGAAGATGAGACAGTAGTGAAACGAATTAAACTG
TTTAAACCTGATTACATATTTGTAGGTATGGGATTCCCTAAACAAGAAGAATGGATTATG
ACACATGAAAACCAATTTGAATCTACAGTGATGATGGGCGTAGGTGGTTCTCTTGAAGTA
TTTGCTGGGGCTAAAAAGAGAGCGCCTTATATCTTTAGAAAATTAAACATTGAATGGATA
TATAGAGCATTAATAGATTGGAAACGTATTGGTAGATTAAAGAGTATTCCAATATTTATG
TATAAAATAGCCAAAGCAAAAAGAAAAATAAAAAAGGCGAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0693 [new locus tag: SACOL_RS03565 ]
- symbol: TagA
- description: teichoic acid biosynthesis protein
- length: 254
- theoretical pI: 9.77365
- theoretical MW: 29132.7
- GRAVY: -0.177953
⊟Function[edit | edit source]
- reaction: EC 2.4.1.187? ExPASyN-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
- TIGRFAM: Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides glycosyltransferase, WecB/TagA/CpsF family (TIGR00696; EC 2.4.1.-; HMM-score: 160.5)
- TheSEED :
- N-acetylmannosaminyltransferase (EC 2.4.1.187)
- PFAM: no clan defined Glyco_tran_WecB; Glycosyl transferase WecB/TagA/CpsF family (PF03808; HMM-score: 173.1)and 1 moreP-loop_NTPase (CL0023) AAA_34; P-loop containing NTP hydrolase pore-1 (PF13872; HMM-score: 11.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002866
- TAT(Tat/SPI): 0.000398
- LIPO(Sec/SPII): 0.000397
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLFIVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDECLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKLFKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIEWIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Michael A D'Elia, James A Henderson, Terry J Beveridge, David E Heinrichs, Eric D Brown
The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureus.
J Bacteriol: 2009, 191(12);4030-4
[PubMed:19376878] [WorldCat.org] [DOI] (I p)