From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0783 [new locus tag: SACOL_RS04025 ]
  • pan locus tag?: SAUPAN002610000
  • symbol: opuBB
  • pan gene symbol?: opuBB
  • synonym:
  • product: osmoprotectant ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0783 [new locus tag: SACOL_RS04025 ]
  • symbol: opuBB
  • product: osmoprotectant ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 805769..807283
  • length: 1515
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACTAACTTTTTCGACATATTGAGTGAACGTAAGGGGCAACTCTTTTCGACAATGATA
    GAACATATTCAAATATCATTTATCGCATTATTGATTGCAACTGCTATTGCGGTACCATTA
    GGTATTTTATTAACGAAGACTAAAACGATATCTGAAATCGTAATGAATATTGCGGCAATT
    CTTCAAACCATACCATCGTTGGCATTATTAGGTTTAATGATTCCTTTATTTGGTATCGGT
    CGTGTGCCAGCAATTATTGCACTTGTAGTGTATGCGTTGTTACCAATTTTAAGGAATACG
    TATACTGGAATTAAAGAAGTTGATCCATCACTCATTGAAGCGGCTAAAGGTATAGGTATG
    AAACCATTTAGACGTTTAACTAAAGTCGAACTTCCGATAGCAATGCCTGTTATAATGGCT
    GGTGTAAGAACGGCTATGGTATTAATTATAGGTACAGCAACACTAGCAGCATTAATTGGT
    GCAGGCGGACTAGGAGATTTAATTTTATTAGGTATAGACCGTAACAATGCATCGTTGATA
    TTATTAGGTGCAATTCCAGCAGCCTTATTGGCAATTATATTTGATTTAATTTTAAGATTT
    ATGGCTAAATTATCTTATAAAAAGTTATTGATGACGTTAGGTGTTATAGTGATGATTATT
    ATACTGGCTATCGCTATTCCTATGTTTGCACAAAAAGGTGATAAAATTACGTTAGCTGGA
    AAGCTTGGCTCCGAGCCATCGATTATTACAAATATGTATAAAATTTTAATAGAAGAAGAG
    ACCAAAAATACTGTAGAAGTGAAAGATGGTATGGGCAAAACAGCATTTTTATTTAATGCT
    TTAAAATCTGACGATATAGATGGGTATTTAGAATTTACTGGAACAGTTTTAGGTGAATTA
    ACAAAAGAACCATTGAAGTCAAAAGAAGAGAAAAAAGTTTATGAACAAGCTAAGCAAAGT
    CTTGAAAAGAAATATCAAATGACTATGTTAAAACCAATGAAGTATAACAATACGTATGCT
    TTAGCTGTAAAACGTGATTTTGCTAAACAACATAATATACGTACAATTGGTGATTTAAAT
    AAGGTTAAAGATCAACTTAAACCAGGATTTACATTGGAATTTAATGATCGTCCAGATGGT
    TACAAAGCTGTTCAAAAGGCTTATAATTTAAATTTAGATAACATACGTACAATGGAACCT
    AAGTTGAGATATCAAGCGATCAATAAAGGTAATATTAATTTAATAGATGCATATTCAACT
    GACGCTGAATTAAAACAATATGATATGGTTGTGTTAAAAGATGATAAGCACGTATTTCCA
    CCATATCAAGGAGCACCATTATTTAAAGAAAGCTTTTTAAAGAAACATCCAGAAATTAAG
    AAACCGTTAAACAAACTAGAAAACAAAATATCTGATGAAGATATGCAAATGATGAACTAT
    AAAGTAACAGTTAAAAATGAAGACCCATATACAGTTGCGAAAGATTATTTAAAAGCAAAA
    GGGTTAATCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1515

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0783 [new locus tag: SACOL_RS04025 ]
  • symbol: OpuBB
  • description: osmoprotectant ABC transporter permease
  • length: 504
  • theoretical pI: 9.9494
  • theoretical MW: 56057.4
  • GRAVY: 0.150794

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 113.1)
    MetabolismTransport and binding proteinsAnionsnitrate ABC transporter, permease protein (TIGR01183; HMM-score: 47)
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 45.8)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 27.5)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 21.3)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 19.6)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 18.2)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 8.4)
  • TheSEED:  
    Choline and Betaine Uptake and Betaine Biosynthesis Glycine betaine ABC transport system, permease protein OpuAB / Glycine betaine ABC transport system, glycine betaine-binding protein OpuAC 
  • PFAM:
    PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 210.3)
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 93.7)
    CYTH (CL0273) Med18; Med18 protein (PF09637; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 69
    • Cmax: 0.119
    • Cmax_pos: 69
    • Smax: 0.156
    • Smax_pos: 1
    • Smean: 0.087
    • D: 0.098
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILLTKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYTGIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIGAGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVMIIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAFLFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMKYNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNLDNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKESFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]