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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00732
  • pan locus tag?: SAUPAN002610000
  • symbol: SAOUHSC_00732
  • pan gene symbol?: opuBB
  • synonym:
  • product: amino acid ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00732
  • symbol: SAOUHSC_00732
  • product: amino acid ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 717232..718746
  • length: 1515
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACTAACTTTTTCGACATATTGAGTGAACGTAAGGGGCAACTCTTTTCGACAATGATA
    GAACATATTCAAATATCATTTATCGCATTATTGATTGCAACTGCTATTGCGGTACCATTA
    GGTATTTTATTAACGAAGACTAAAACGATATCTGAAATCGTAATGAATATTGCGGCAATT
    CTTCAAACCATACCATCGTTGGCATTATTAGGTTTAATGATTCCTTTATTTGGTATCGGT
    CGTGTGCCAGCAATTATTGCACTTGTAGTGTATGCGTTGTTACCAATTTTAAGGAATACG
    TATACTGGAATTAAAGAAGTTGATCCATCACTCATTGAAGCGGCTAAAGGTATAGGTATG
    AAACCATTTAGACGTTTAACTAAAGTCGAACTTCCGATAGCAATGCCTGTTATAATGGCT
    GGTGTAAGAACGGCTATGGTATTAATTATAGGTACAGCAACACTAGCAGCATTAATTGGT
    GCAGGCGGACTAGGAGATTTAATTTTATTAGGTATAGACCGTAACAATGCATCGTTGATA
    TTATTAGGTGCAATTCCAGCAGCCTTATTGGCAATTATATTTGATTTAATTTTAAGATTT
    ATGGCTAAATTATCTTATAAAAAGTTATTGATGACGTTAGGTGTTATAGTGATGATTATT
    ATACTGGCTATCGCTATTCCTATGTTTGCACAAAAAGGTGATAAAATTACGTTAGCTGGA
    AAGCTTGGCTCCGAGCCATCGATTATTACAAATATGTATAAAATTTTAATAGAAGAAGAG
    ACCAAAAATACTGTAGAAGTGAAAGATGGTATGGGCAAAACAGCATTTTTATTTAATGCT
    TTAAAATCTGACGATATAGATGGGTATTTAGAATTTACTGGAACAGTTTTAGGTGAATTA
    ACAAAAGAACCATTGAAGTCAAAAGAAGAGAAAAAAGTTTATGAACAAGCTAAGCAAAGT
    CTTGAAAAGAAATATCAAATGACTATGTTAAAACCAATGAAGTATAACAATACGTATGCT
    TTAGCTGTAAAACGTGATTTTGCTAAACAACATAATATACGTACAATTGGTGATTTAAAT
    AAGGTTAAAGATCAACTTAAACCAGGATTTACATTGGAATTTAATGATCGTCCAGATGGT
    TACAAAGCTGTTCAAAAGGCTTATAATTTAAATTTAGATAACATACGTACAATGGAACCT
    AAGTTGAGATATCAAGCGATCAATAAAGGTAATATTAATTTAATAGATGCATATTCAACT
    GACGCTGAATTAAAACAATATGATATGGTTGTGTTAAAAGATGATAAGCACGTATTTCCA
    CCATATCAAGGAGCACCATTATTTAAAGAAAGCTTTTTAAAGAAACATCCAGAAATTAAG
    AAACCGTTAAACAAACTAGAAAACAAAATATCTGATGAAGATATGCAAATGATGAACTAT
    AAAGTAACAGTTAAAAATGAAGACCCATATACAGTTGCGAAAGATTATTTAAAAGCAAAA
    GGGTTAATCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1515

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00732
  • symbol: SAOUHSC_00732
  • description: amino acid ABC transporter permease
  • length: 504
  • theoretical pI: 9.9494
  • theoretical MW: 56057.4
  • GRAVY: 0.150794

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 113.1)
    MetabolismTransport and binding proteinsAnionsnitrate ABC transporter, permease protein (TIGR01183; HMM-score: 47)
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 45.8)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 27.5)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 21.3)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 19.6)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 18.2)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 8.4)
  • TheSEED:  
    Choline and Betaine Uptake and Betaine Biosynthesis Glycine betaine ABC transport system, permease protein OpuAB / Glycine betaine ABC transport system, glycine betaine-binding protein OpuAC 
  • PFAM:
    PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 210.3)
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 93.7)
    CYTH (CL0273) Med18; Med18 protein (PF09637; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 69
    • Cmax: 0.119
    • Cmax_pos: 69
    • Smax: 0.156
    • Smax_pos: 1
    • Smean: 0.087
    • D: 0.098
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILLTKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYTGIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIGAGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVMIIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAFLFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMKYNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNLDNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKESFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]