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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0908 [new locus tag: SACOL_RS04660 ]
  • pan locus tag?: SAUPAN002880000
  • symbol: SACOL0908
  • pan gene symbol?: ear
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0908 [new locus tag: SACOL_RS04660 ]
  • symbol: SACOL0908
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 917356..917913
  • length: 558
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATAAAAAACTATTAACAAGAACATTGATAGCAAGTGCTTTAGTTTTAACAACAGTA
    GGTTCAGGTTTTCATTCTTCTTCAAATTATAATGGTATTAATAACGTTGCAAAGGCTTCT
    GAAATAACAGATAGAGACTTATGGAAAAATGTAAGAGATGCTTTGAAAGAAGCAAATATT
    ATCGATAAAACAGCAAATGAGACAGTTGGTGTTACGTACAATTTAAATAATGGTGGCGAA
    AGTAGTATTACGGGTACAGCTGATTTAGATGAACTTAGTAATTTTAATAACAAGCCGATT
    AATACCGATAGTGTTAAAAGAATTGATTTGTCAAGAATAAATCCAAATGGAAATAGGTTT
    GACGCAAATGATGCATGGAAAAAATTAACTGACAAATTAAAAGAGAAACAAATCGTTAAA
    AACGGCGACACAGTAACTATCCATAGTAAAGATAAAAATGATCCCCCAATTTCAGCTAAA
    GTTGGTGAAAACTATAATGGCAACAAGGGGTTAATGCTAAATCAGAGAGATATAACAAAA
    ATAACTATAACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    558

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0908 [new locus tag: SACOL_RS04660 ]
  • symbol: SACOL0908
  • description: hypothetical protein
  • length: 185
  • theoretical pI: 9.99344
  • theoretical MW: 20345.7
  • GRAVY: -0.692432

Function[edit source | edit]

  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsputative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 17.9)
  • TheSEED:  
    Phages, Prophages, Transposable elements, PlasmidsPathogenicity islandsStaphylococcal pathogenicity islands SaPI Secreted protein Ear, superantigen-encoding pathogenicity islands SaPI 
  • PFAM:
    no clan definedDUF4888; Domain of unknown function (DUF4888) (PF16229; HMM-score: 75.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.364
    • Ymax_pos: 27
    • Cmax: 0.272
    • Cmax_pos: 40
    • Smax: 0.924
    • Smax_pos: 11
    • Smean: 0.843
    • D: 0.55
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKKLLTRTLIASALVLTTVGSGFHSSSNYNGINNVAKASEITDRDLWKNVRDALKEANIIDKTANETVGVTYNLNNGGESSITGTADLDELSNFNNKPINTDSVKRIDLSRINPNGNRFDANDAWKKLTDKLKEKQIVKNGDTVTIHSKDKNDPPISAKVGENYNGNKGLMLNQRDITKITITK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL0908 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence;RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]