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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1025 [new locus tag: SACOL_RS05230 ]
  • pan locus tag?: SAUPAN003199000
  • symbol: prfC
  • pan gene symbol?: prfC
  • synonym:
  • product: peptide chain release factor 3

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1025 [new locus tag: SACOL_RS05230 ]
  • symbol: prfC
  • product: peptide chain release factor 3
  • replicon: chromosome
  • strand: +
  • coordinates: 1033818..1035380
  • length: 1563
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAACTTAAAGCAAGAAGTTGAGTCTAGAAAGACTTTTGCGATTATTTCACATCCCGAT
    GCAGGGAAAACAACGTTAACTGAAAAACTATTGTACTTCAGTGGTGCTATTCGTGAAGCG
    GGTACAGTTAAAGGGAAGAAGACTGGTAAATTTGCGACAAGTGACTGGATGAAAGTTGAA
    CAAGAGCGTGGTATTTCTGTAACTAGTTCAGTAATGCAATTTGATTACGATGATTATAAA
    ATCAATATCTTAGATACACCAGGACATGAAGACTTTTCAGAAGATACGTATAGAACATTA
    ATGGCAGTTGACAGTGCTGTCATGGTCATAGACTGTGCAAAAGGTATTGAACCACAAACA
    TTGAAGTTATTTAAAGTTTGTAAAATGCGTGGTATTCCAATCTTTACATTCATTAATAAA
    TTAGACCGAGTAGGTAAAGAACCATTTGAATTATTAGATGAAATCGAAGAGACATTAAAT
    ATTGAAACATACCCTATGAATTGGCCAATTGGTATGGGACAAAGTTTCTTTGGCATCATT
    GATAGAAAGTCTAAAACAATTGAACCATTTAGAGATGAAGAAAATATATTACATTTGAAT
    GATGATTTTGAGTTGGAAGAAGATCATGCAATTACAAATGATAGTGATTTTGAACAAGCG
    ATTGAAGAATTAATGTTGGTTGAAGAAGCGGGTGAAGCCTTTGATAATGACGCGCTGTTG
    AGTGGAGACTTAACACCTGTATTTTTCGGTTCAGCTTTAGCTAACTTTGGTGTACAAAAT
    TTCTTAAATGCATATGTTGATTTTGCGCCAATGCCAAATGCGAGACAAACAAAAGAAGAC
    GTTGAAGTAAGCCCGTTTGATGATTCATTTTCAGGATTTATCTTTAAAATTCAAGCCAAC
    ATGGACCCTAAACACCGTGATAGAATTGCCTTTATGCGTGTCGTTAGTGGTGCATTTGAA
    CGTGGTATGGATGTTACTTTGCAACGTACTAATAAAAAGCAAAAGATCACACGTTCAACG
    TCATTTATGGCAGACGATAAAGAAACTGTGAATCATGCTGTAGCAGGCGATATCATTGGA
    CTATATGATACTGGTAATTATCAAATTGGAGATACTTTAGTTGGTGGAAAACAAACCTAC
    AGTTTCCAAGATTTACCACAATTTACGCCAGAAATTTTTATGAAAGTTTCTGCTAAAAAC
    GTCATGAAACAGAAGCATTTCCATAAAGGTATTGAACAATTAGTACAAGAAGGTGCGATT
    CAATACTATAAAACATTACACACAAACCAAATTATTTTAGGTGCTGTTGGTCAGTTACAA
    TTTGAAGTTTTCGAACATAGAATGAAAAACGAATATAATGTTGATGTTGTTATGGAGCCA
    GTAGGCCGTAAAATTGCACGTTGGATTGAAAATGAAGACCAAATTACAGATAAGATGAAC
    ACATCAAGATCGATTTTAGTGAAAGATAGATATGACGATTTAGTATTCTTATTTGAAAAT
    GAATTTGCAACAAGATGGTTTGAAGAGAAATTCCCTGAAATTAAATTGTATAGTTTACTT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1563

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1025 [new locus tag: SACOL_RS05230 ]
  • symbol: PrfC
  • description: peptide chain release factor 3
  • length: 520
  • theoretical pI: 4.61861
  • theoretical MW: 59601.2
  • GRAVY: -0.390577

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisTranslation factorspeptide chain release factor 3 (TIGR00503; HMM-score: 647.7)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor G (TIGR00484; HMM-score: 250.4)
    Cellular processesCellular processesAdaptations to atypical conditionsGTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Genetic information processingProtein synthesisTranslation factorsGTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Signal transductionRegulatory functionsOtherGTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Unknown functionGeneralelongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 123.4)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor aEF-2 (TIGR00490; HMM-score: 117.3)
    Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 88.2)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 66.5)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor Tu (TIGR00485; HMM-score: 61.2)
    Genetic information processingProtein synthesisTranslation factorsselenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 55.5)
    Genetic information processingProtein synthesisTranslation factorstranslation initiation factor IF-2 (TIGR00487; HMM-score: 48.7)
    MetabolismCentral intermediary metabolismSulfur metabolismsulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 45.8)
    Genetic information processingProtein synthesisTranslation factorstranslation initiation factor aIF-2 (TIGR00491; HMM-score: 40.5)
    Genetic information processingProtein synthesisOtherribosome-associated GTPase EngA (TIGR03594; HMM-score: 28.3)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 24.6)
    Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 17.7)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 12.2)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 11.6)
  • TheSEED:  
    Protein MetabolismProtein biosynthesisTranslation termination factors bacterial Peptide chain release factor 3 
  • PFAM:
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 187.3)
    EF-G_C (CL0437) RF3_C; Class II release factor RF3, C-terminal domain (PF16658; HMM-score: 154.7)
    EFTPs (CL0575) GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 48.1)
    EF-G_C (CL0437) EFG_II; Elongation Factor G, domain II (PF14492; HMM-score: 36.3)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 30.3)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 17.9)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 11
    • Cmax: 0.133
    • Cmax_pos: 46
    • Smax: 0.359
    • Smax_pos: 1
    • Smean: 0.226
    • D: 0.186
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 91 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

A M Ludovice, S W Wu, H de Lencastre
Molecular cloning and DNA sequencing of the Staphylococcus aureus UDP-N-acetylmuramyl tripeptide synthetase (murE) gene, essential for the optimal expression of methicillin resistance.
Microb. Drug Resist.: 1998, 4(2);85-90
[PubMed:9650993] [WorldCat.org] [DOI] (P p)