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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1096 [new locus tag: SACOL_RS05595 ]
  • pan locus tag?: SAUPAN003308000
  • symbol: SACOL1096
  • pan gene symbol?: ktrA
  • synonym:
  • product: TrkA potassium uptake family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1096 [new locus tag: SACOL_RS05595 ]
  • symbol: SACOL1096
  • product: TrkA potassium uptake family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1106950..1107612
  • length: 663
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGGTAAAGAATATGTAGTCATCGGTCTAGGTCGTTTTGGAGGTAGTATCGTTCGTGAG
    TTGAATGCATTAGACATGGATGTAATGGCCATCGACCATGATGAAAATAGAGTGAATGAA
    TATAGTGATATCGCTACTCATGCGGTTGTTGCAGACACTACAGATGAAGCAGTTATGAAA
    AGTTTAGGTATCCGTAACTTTGATCATGTCATTGTGGCAATTGGTGAAAATATTCAATCA
    AGTACGTTGACGACTTTAATTTTAAAAGAGTTAGGTGTAAAAAAAGTAACTGCTAAAGCA
    CAAAATGATTATCATGCAAAGATTTTAAATAAAATTGGAGCAGATACGGTTGTGCACCCT
    GAGCGTGATATGGGTAGACGTATTGCGCATAATGTTGCGAGTGCAAGTGTACTTGATTAT
    CTTGAGTTGGCAGACGAGCATTCTATTGTAGAATTGAAAGCAACTGAAAAGATGGCGGGG
    CAGTCTATCATTGATTTAGATATAAGAGCACAATATGGAATTAACATTATTGCAATTAAA
    CGAGGCAAAGAGTTCATCATTTCACCAAATCCAAATATTAATTTAGAAATAGGTGATATT
    TTAATCATGATTGGACATGATAATGATTTAAATCGCTTTGAAAAAAATATTGCGACGAGA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    663

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1096 [new locus tag: SACOL_RS05595 ]
  • symbol: SACOL1096
  • description: TrkA potassium uptake family protein
  • length: 220
  • theoretical pI: 5.32766
  • theoretical MW: 24282.6
  • GRAVY: -0.08

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    aspartate-alanine antiporter (TIGR03802; HMM-score: 19.5)
    MetabolismEnergy metabolismPentose phosphate pathway6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 16.2)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 16)
    MetabolismEnergy metabolismAmino acids and aminesL-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 15.1)
    Genetic information processingProtein fateProtein folding and stabilizationxanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 14.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOtherxanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 14.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 13.6)
    Unknown functionEnzymes of unknown specificityputative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 13.2)
  • TheSEED:  
    Bacterial RNA-metabolizing Zn-dependent hydrolases KtrCD potassium uptake system, peripheral membrane component KtrC 
    Conserved gene cluster associated with Met-tRNA formyltransferase KtrCD potassium uptake system, peripheral membrane component KtrC 
    Glutathione-regulated potassium-efflux system and associated functions KtrCD potassium uptake system, peripheral membrane component KtrC 
    Hyperosmotic potassium uptake KtrCD potassium uptake system, peripheral membrane component KtrC 
    Potassium homeostasis KtrCD potassium uptake system, peripheral membrane component KtrC 
  • PFAM:
    NADP_Rossmann (CL0063) TrkA_N; TrkA-N domain (PF02254; HMM-score: 104.5)
    TrkA_C (CL0582) TrkA_C; TrkA-C domain (PF02080; HMM-score: 54.6)
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 22.9)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 21.7)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 20.5)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 16.9)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 15.6)
    NmrA; NmrA-like family (PF05368; HMM-score: 14)
    XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 13.6)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 11.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.179
    • Ymax_pos: 31
    • Cmax: 0.189
    • Cmax_pos: 31
    • Smax: 0.234
    • Smax_pos: 25
    • Smean: 0.125
    • D: 0.158
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDIATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQNDYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAGQSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIATR

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 117 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]