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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1500 [new locus tag: SACOL_RS07640 ]
  • pan locus tag?: SAUPAN003920000
  • symbol: SACOL1500
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1500 [new locus tag: SACOL_RS07640 ]
  • symbol: SACOL1500
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1541508..1542215
  • length: 708
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    GTGAGTTTATTGTCTTCAATTTTTACTATGATAATTTATTTCGTAATATTAATGGTGCTT
    CCTTTATGGGCACAACACAAAGTTAAATCTAACTATGAGAAATATTCACAAGTTAGATCA
    ACTAGTGGTAAAACGGGTCGTGAAGTTGCATTAGAAATTTTACATGCAAACGGTATTTAT
    GATGTCGAAGTTGTTAAAGGTGAAGGATTCTTAACAGATCATTACGATCCTAAAAAGAAA
    GTTGTTTCACTATCTCCTGCAAACTATGACAGACCATCAGTTGCTGGAACTGCAATTGCA
    GCCCACGAAGTTGGTCATGCCATTCAAGATCACCAAGGATATTGGTTCTTAAGATTCAGA
    GCAGCATTAGTACCTGTTGCAAACCTAGGGAGTTCATTAAGTTATATGATCATCATGTTA
    GGTATTATCTTAACAGCGATAGGTAGTGCATTTGGTTCAACTGCTTTATGGATTGGTGCT
    GGTTTAATGTCATTAGCGGTATTATTCTCAATTGTTACGTTACCAGTTGAGTTTGATGCG
    AGTTCAAGAGCGATGAAACAAATTACGGCATTGAACATTGTAAATGAAAAAGAATACAAG
    CATGCGCGTAAAGTATTATCTGCAGCAGCAATGACTTATGTTGCAGCAACTGCAGTCGCT
    GTAGCAGAGCTTGTACGTATTATTTTGATTGCGCGTTCAAGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    708

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1500 [new locus tag: SACOL_RS07640 ]
  • symbol: SACOL1500
  • description: hypothetical protein
  • length: 235
  • theoretical pI: 9.59502
  • theoretical MW: 25561.7
  • GRAVY: 0.448936

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (TIGR03074; EC 1.1.-.-; HMM-score: 6.4)
  • TheSEED:  
    probable metal-dependent peptidase 
  • PFAM:
    Peptidase_MA (CL0126) Zn_peptidase_2; Putative neutral zinc metallopeptidase (PF04298; HMM-score: 295)
    DUF4157; Domain of unknown function (DUF4157) (PF13699; HMM-score: 16.5)
    GPCR_A (CL0192) DUF3522; Protein of unknown function (DUF3522) (PF12036; HMM-score: 9.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.3
    • Ymax_pos: 25
    • Cmax: 0.246
    • Cmax_pos: 25
    • Smax: 0.587
    • Smax_pos: 23
    • Smean: 0.337
    • D: 0.315
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSLLSSIFTMIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEILHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDHQGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLFSIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRIILIARSSD

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]