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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1639 [new locus tag: SACOL_RS08355 ]
  • pan locus tag?: SAUPAN004165000
  • symbol: hrcA
  • pan gene symbol?: hrcA
  • synonym:
  • product: heat-inducible transcription repressor HrcA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1639 [new locus tag: SACOL_RS08355 ]
  • symbol: hrcA
  • product: heat-inducible transcription repressor HrcA
  • replicon: chromosome
  • strand: -
  • coordinates: 1669133..1670110
  • length: 978
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTACAGATAGGCAATTGAGTATATTAAACGCAATTGTTGAGGATTATGTTGATTTT
    GGACAACCCGTTGGTTCTAAAACACTAATTGAGCGACATAACTTGAATGTTAGTCCTGCT
    ACAATTAGAAATGAGATGAAACAGCTTGAAGATTTAAACTATATCGAGAAGACACATAGT
    TCTTCAGGGCGTTCGCCATCACAATTAGGTTTTAGGTATTATGTCAATCGTTTACTTGAA
    CAAACATCTCATCAAAAAACAAATAAATTAAGACGATTAAATCAATTGTTAGTTGAGAAT
    CAATATGATGTATCATCAGCATTGACATATTTTGCAGATGAATTATCAAATATATCTCAA
    TATACAACTTTAGTTGTTCATCCTAATCATAAACAAGATATTATCAATAATGTACACTTG
    ATTCGTGCTAATCCTAATTTAGTTATAATGGTTATCGTATTTTCATCAGGTCATGTTGAG
    CATGTACATCTTGCTTCAGACATACCGTTTAATAATGACAAACTAAATACAATATCAAAT
    TTTGTTACAAACAAACTAACTGAGTTTAATCAGAATTTACAAGATGATATTGTCTCTTTT
    GTTCAATCAGAGCAGGAAGAAATTTTTATCAATAAATTGTTAAATACGATGAATAATCAT
    ATTTCTAATCAAAGTAATAGCATTTATATGGGTGGAAAGGTTAAACTTATTGATGCATTG
    AATGAAAGTAATGTATCTTCAATTCAGCCCATTCTACAATATATAGAATCAAATAGAATT
    GCAGAATTATTACAAGATATATCTTCACCAAATATTAATGTAAAAATTGGTAATGAAATT
    GATGACAGCTTAAGTGATATTTCAATTGTAACAAGTCAATATCATTTTGATGAAACGTTA
    AAAGGTCAAATCGCTGTGATTGGACCTACAGCTATGCATTATCAAAATGTCATTCAATTA
    TTAAATAGAATTTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1639 [new locus tag: SACOL_RS08355 ]
  • symbol: HrcA
  • description: heat-inducible transcription repressor HrcA
  • length: 325
  • theoretical pI: 5.71442
  • theoretical MW: 37015.4
  • GRAVY: -0.301231

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsheat-inducible transcription repressor HrcA (TIGR00331; HMM-score: 345.7)
  • TheSEED:  
    Stress ResponseHeat shockHeat shock dnaK gene cluster extended Heat-inducible transcription repressor HrcA 
  • PFAM:
    GAF (CL0161) HrcA; HrcA protein C terminal domain (PF01628; HMM-score: 138.5)
    HTH (CL0123) HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 18.7)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 18.2)
    HTH_11; HTH domain (PF08279; HMM-score: 16.7)
    no clan definedFOXO_KIX_bdg; KIX-binding domain of forkhead box O, CR2 (PF16675; HMM-score: 13.3)
    HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 12.5)
    LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 12.4)
    Tubulin_C (CL0442) FtsZ_C; FtsZ family, C-terminal domain (PF12327; HMM-score: 12.3)
    HTH (CL0123) Ribosomal_S19e; Ribosomal protein S19e (PF01090; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 27
    • Cmax: 0.126
    • Cmax_pos: 27
    • Smax: 0.143
    • Smax_pos: 39
    • Smean: 0.084
    • D: 0.102
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRNEMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQYDVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVEHVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMNNHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKIGNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: HrcA
    HrcA (TF) important in Heat shock responseRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]