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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1739 [new locus tag: SACOL_RS08890 ]
  • pan locus tag?: SAUPAN004316000
  • symbol: SACOL1739
  • pan gene symbol?: phoR
  • synonym:
  • product: sensory box histidine kinase PhoR

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1739 [new locus tag: SACOL_RS08890 ]
  • symbol: SACOL1739
  • product: sensory box histidine kinase PhoR
  • replicon: chromosome
  • strand: -
  • coordinates: 1772699..1774363
  • length: 1665
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGAAGTTTCACCACCGCTTAATGTTATTGATAAGCAGTATATTAATTATCAGTTTT
    GTTACATTAGGCATTATTATTCACGCTTCCATCACATATGTTTTATCAGAAAAACACGAA
    CAAGCTCTAGCAACAGATGCAAGATCATATATTTATTTAGTCCAAGATAATGAGATTGAT
    AAAGTTAAAAAAATTGTAAAACAGCAAAATATAGATTTATTGATAACTAAGCATGATAAA
    AAGGTGTTTAGTAGTGGCAAACTGAAAGCTATCCCAAATAGTGATAGCTATATTCAAAAA
    AGGGACGTTTTTATTTTTAATAAGAAGGTTAACGGTTATCATGTATGGATTAAAGGTTAC
    AATAACGATATTACAGAAATGCATTGGACGTTGTGGAAATATCTTATTTTGACATGTTTA
    GTTGTACTCATTTGTCTGTATTTTGCAAGCCGTAGTTTTAAGCGTACATTAATCCGTCCC
    ATTCAAGAAGTTACTTATGCAACACAATTGTTGGCAAATGGTTATTATCACATACGTGTA
    CCTGAAAGTAATGTTGTAGAAACTAAAGCGCTTTTTGTGTCTACCAATGATTTAGCACGA
    CGCCTTCAAAAGCTTAATAATGAGCAAAAAATTCAATCCAACCGTTTAAAAACAACGATT
    GAAAATATTCCAAGTGCTATTTTAATGATAGATCGAAATGGAAAAATCGTTGTTGCAAAT
    AAAGCGTATTATGAACAGTTCAACATCTCGCATAATATTGAACAAGTAGGCTATCACGGC
    TATGTTAATACTGAAATTGAGCAATTAATTTTAGAAAGTTTTAAAGTTGAAAAACCTATT
    TATGAACAATTGGAAGTTGCAATAAACCAAGTCCATGCCAAGTACTTTGATATTTCATGC
    GTTCCGATTTTAACTAGGTCTCAAAAAAGTTTGCAAGGGATTTTAGTTGTGATGCATGAT
    ATAACAAATCTGAAACAACTTGAAAATCTAAGACGTGAATTTGTAGCAAATGTTTCACAT
    GAATTAAAAACACCTATTACTTCAATTAAGGGGTTTGCTGAAACGCTTATTGATGGTGCA
    AAAAATGATGCGGAATCATTAGACATGTTTTTAAACATAATTTTAAAAGAATCAAATCGA
    ATAGAATCTTTAGTAACAGATCTGTTAGATTTATCGCATATTGAGCAACATACAGAGTTA
    GATACAGACTATATGAATTTATCAGATTTAACGCGGCGTATTATTGATAATATGATGACT
    CAAGCCAATCAAAAAAATATTTCCATTCATACTGATATTGAAAAAGATGTCATTGTTAAA
    GCACAAGAAAGTAAAATTGCTCAAGTTATCACGAATTTGTTAACGAATGCAATTAACTAT
    TCTTATGAAGATGGAGATATTAACGTTCGTGTGTATCGAGATGACTTTCGTGTCATTTTC
    GAAGTACAAGATTTTGGTATAGGTATTAAATTAGAAGACCAACAACGTATTTTTGAAAGA
    TTTTATAGAGTAGATAAAGCACGAAGTAGAGATTCTGGTGGGACAGGTCTTGGATTATCA
    ATTACAAAGCATATAGTAGAGGCCCACCAAGGCAATATTGAAGTGAATAGTCAAGTTGGC
    AAAGGCTCAACGTTCAAAGTTATTCTAAAAGATTATAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1665

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1739 [new locus tag: SACOL_RS08890 ]
  • symbol: SACOL1739
  • description: sensory box histidine kinase PhoR
  • length: 554
  • theoretical pI: 8.11061
  • theoretical MW: 63904.1
  • GRAVY: -0.198375

Function[edit source | edit]

  • reaction:
    ATP + protein L-histidine = ADP + protein N-phospho-L-histidine?
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 307.5)
    Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 149.9)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 142.3)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 70.8)
    MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
    Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
    Signal transductionRegulatory functionsSmall molecule interactionsPAS domain S-box protein (TIGR00229; HMM-score: 27.8)
    MetabolismEnergy metabolismPhotosynthesistranscriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryHigh affinity phosphate transporter and control of PHO regulon Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) 
    Phosphorus Metabolism - no subcategoryPhosphate metabolism Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) 
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 91)
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 70.5)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 27.1)
    PAS_4; PAS fold (PF08448; HMM-score: 25)
    PAS_7; PAS fold (PF12860; HMM-score: 22.5)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 20.4)
    PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 18.6)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 18.4)
    PAS_Fold (CL0183) PAS_8; PAS domain (PF13188; HMM-score: 17.7)
    no clan definedSecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 14.1)
    MKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.247
    • Ymax_pos: 11
    • Cmax: 0.219
    • Cmax_pos: 45
    • Smax: 0.683
    • Smax_pos: 1
    • Smean: 0.603
    • D: 0.386
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALATDARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDVFIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPIQEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTTIENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVEKPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSHIEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARSRDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]